Immunohistochemical identification of collagen in the equine lung.
Abstract: Recurrent airway obstruction (RAO) in the horse is a disease characterized by reversible bronchoconstriction and by mucus and neutrophil accumulation in the airways. It has been hypothesized that in horses with RAO, remodeling changes occur that are similar to those described in humans with asthma. Although collagen fibrils are present surrounding normal airways, they are a prominent feature of airway remodeling in human asthma with evidence of enhanced collagen III and I fibril deposition. An immunolabeling method was developed to identify collagen I and III in equine lung and to describe the collagen fiber type and distribution within the walls of the noncartilagenous bronchioles. The health status of 14 horses was characterized by clinical respiratory exam, bronchoalveolar lavage cytology, and pulmonary function tests. Following postmortem examination and histological assessment, horses were divided into RAO-affected (n = 4) and nonaffected (n = 10) groups. Eight sections per horse from all lung regions were evaluated histologically. Results of the study showed that collagens I and III were present in the lamina propria and adventitial area of the noncartilaginous bronchioles. There was clear staining differentiation between collagen I or III, airway smooth muscle, and the airway epithelium. Collagen I and III were present in the lamina propria and adventitial areas of the noncartilaginous bronchioles of horses, and there was no significant difference in the relative amount of collagen I and III between this group of RAO-affected and nonaffected horses.
Publication Date: 2010-06-22 PubMed ID: 20571145DOI: 10.1177/0300985810375051Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigated the presence and distribution of collagen types I and III in the lungs of horses, specifically in those with and without a condition called Recurrent Airway Obstruction (RAO), akin to human asthma. The research did not find significant differences in the amounts of collagen types I and III between horses with RAO and their healthy counterparts.
Objective and Methodology
- The study aimed to identify the collagen type and its distribution in the lungs of horses, particularly in those affected with RAO. This condition characterizes recurring constriction of the bronchial tubes, as well as the accumulation of mucus and neutrophils.
- An immunolabeling method was used to identify collagen types I and III in horse lungs as these collagens are a crucial part of the normal airway structure and have been found to be involved in airway remodeling in human asthma.
- The health of 14 horses was evaluated through clinical respiratory exams, bronchoalveolar lavage cytology, and pulmonary function tests. Postmortem examinations and histological assessments were performed, dividing the specimens into those from RAO-affected horses and nonaffected ones.
Findings
- Results showed that collagen types I and III were present in specific areas of the noncartilaginous bronchioles – the lamina propria and adventitial area respectively.
- Differentiation could clearly be made between these collagen types, airway smooth muscle, and airway epithelium through staining, which indicated a precise localization of these components.
- The presence of Collagen I and III in the respiratory tract of horses was easily identified, both in the RAO-affected and nonaffected horses.
- However, the study did not reveal a significant variance in the relative amount of either collagen type between the group of RAO-affected and nonaffected horses.
Conclusion
- While collagen fibers, particularly types I and III, are a notable part of airway remodeling in human asthma, this study did not find considerable differences in their presence in horses suffering from RAO compared to healthy horses.
- This finding suggests that airway remodeling in horses with RAO may not be directly linked to collagen deposition, unlike in human asthma.
Cite This Article
APA
Furness MC, Bienzle D, Caswell JL, Delay J, Viel L.
(2010).
Immunohistochemical identification of collagen in the equine lung.
Vet Pathol, 47(5), 982-990.
https://doi.org/10.1177/0300985810375051 Publication
Researcher Affiliations
- Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
MeSH Terms
- Airway Obstruction / metabolism
- Airway Obstruction / veterinary
- Animals
- Bronchoalveolar Lavage Fluid / cytology
- Collagen Type I / metabolism
- Collagen Type III / metabolism
- Female
- Horse Diseases / metabolism
- Horses
- Immunohistochemistry / veterinary
- Lung Diseases / metabolism
- Lung Diseases / veterinary
- Male
- Respiratory Function Tests / veterinary
- Statistics, Nonparametric
Citations
This article has been cited 1 times.- de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG. Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma. Biomed Res Int 2015;2015:893485.
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