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Reproduction in domestic animals = Zuchthygiene2007; 42(5); 516-526; doi: 10.1111/j.1439-0531.2006.00815.x

Immunohistochemical localization of vascular endothelial growth factor (VEGF) and its two receptors (Flt-I and KDR) in the endometrium and placenta of the mare during the oestrous cycle and pregnancy.

Abstract: Polyclonal antisera for vascular endothelial growth factor (VEGF) and its two main receptor molecules, VEGF-I (Flt) and VEGF-II (KDR), were used in a conventional immunocytochemical staining method to localize these angiogenic ligand molecules in the endometrium and placenta of the mare during the oestrous cycle and pregnancy. The anti-VEGF and anti-Flt sera both labelled the lumenal and glandular epithelia of the endometrium throughout the oestrous cycle and both the invasive trophoblast cells of the endometrial cups and the non-invasive trophoblast of the allantochorion in pregnancy. The anti-KDR serum likewise stained the maternal and foetal epithelial layers during the oestrous cycle and pregnancy and it also labelled fibroblast-like cells in the endometrial and allantoic stromas and the endothelium of foetal and maternal capillaries. The results demonstrated that constant supplies of the principal vasculogenic and angiogenic factor, VEGF, and its two major receptors, Flt and KDR, are available on both the maternal and foetal sides of the placental barrier throughout gestation in the mare. They are presumed to facilitate the continuing development of the extensive foetal and maternal capillary networks that are such prominent features within the microplacentomes of the diffuse, epitheliochorial equine placenta.
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Publication Date: 2007-09-12 PubMed ID: 17845608DOI: 10.1111/j.1439-0531.2006.00815.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the presence and location of a vital growth factor, VEGF, and its two receptors in the endometrium and placenta of mares, throughout different stages such as the oestrous cycle and pregnancy. The growth factor and its receptors are believed to play a significant role in the development of capillary networks vital for pregnancy.

Objective and Methodology

  • The researchers’ aim was to identify the location of the essential angiogenic molecules (VEGF, Flt, and KDR) in the endometrium and placenta of mares during the oestrous cycle and pregnancy.
  • Polyclonal antisera for VEGF and its two primary receptor molecules, VEGF-I (Flt) and VEGF-II (KDR), were used in the study. This is achieved through a conventional immunocytochemical staining method.

Findings

  • The anti-VEGF and anti-Flt sera labelled the lumenal and glandular epithelia of the endometrium throughout the oestrous cycle. They also labelled the invasive trophoblast cells of the endometrial cups and the non-invasive trophoblast of the allantochorion during pregnancy.
  • The anti-KDR serum similarly stained the maternal and foetal epithelial layers during the oestrous cycle and pregnancy, and it also labelled fibroblast-like cells in the endometrial and allantoic stromas and the endothelium of foetal and maternal capillaries.
  • The results showed that the primary vasculogenic and angiogenic factor, VEGF, and its two significant receptors, Flt and KDR, are continuously present on both the maternal and foetal sides of the placental barrier throughout gestation in the mare.

Implications

  • This research implies that the constant supply of VEGF and its receptors play a crucial role in foetal and maternal capillary network development. These networks are a distinctive feature of the diffuse, epitheliochorial equine placenta.
  • The research findings help to better understand the role and function of VEGF and its two primary receptors, Flt, and KDR, during the oestrous cycle and pregnancy in mares.

Cite This Article

APA
Allen WR, Gower S, Wilsher S. (2007). Immunohistochemical localization of vascular endothelial growth factor (VEGF) and its two receptors (Flt-I and KDR) in the endometrium and placenta of the mare during the oestrous cycle and pregnancy. Reprod Domest Anim, 42(5), 516-526. https://doi.org/10.1111/j.1439-0531.2006.00815.x

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 0936-6768
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 42
Issue: 5
Pages: 516-526

Researcher Affiliations

Allen, W R
  • Department of Veterinary Medicine Equine Fertility Unit, University of Cambridge, Mertoun Paddocks, Woodditton Road, Newmarket, Suffolk, UK. efu@tesco.net
Gower, S
    Wilsher, S

      MeSH Terms

      • Animals
      • Endometrium / chemistry
      • Endothelium, Vascular / chemistry
      • Estrus / physiology
      • Female
      • Horses / physiology
      • Immunohistochemistry / veterinary
      • Neovascularization, Physiologic
      • Placenta / chemistry
      • Pregnancy
      • Pregnancy, Animal / physiology
      • Receptors, Vascular Endothelial Growth Factor / analysis
      • Receptors, Vascular Endothelial Growth Factor / metabolism
      • Vascular Endothelial Growth Factor A / analysis
      • Vascular Endothelial Growth Factor A / metabolism

      Citations

      This article has been cited 12 times.
      1. Martinez RE, Leatherwood JL, Bradbery AN, Paris BL, Hammer CJ, Kelley D, Bazer FW, Wu G. Evaluation of dietary arginine supplementation to increase placental nutrient transporters in aged mares. Transl Anim Sci 2023 Jan;7(1):txad058.
        doi: 10.1093/tas/txad058pubmed: 37593152google scholar: lookup
      2. Ellero N, Lanci A, Baldassarro VA, Alastra G, Mariella J, Cescatti M, Castagnetti C, Giardino L. Study on NGF and VEGF during the Equine Perinatal Period-Part 2: Foals Affected by Neonatal Encephalopathy. Vet Sci 2022 Aug 26;9(9).
        doi: 10.3390/vetsci9090459pubmed: 36136675google scholar: lookup
      3. Ellero N, Lanci A, Baldassarro VA, Alastra G, Mariella J, Cescatti M, Giardino L, Castagnetti C. Study on NGF and VEGF during the Equine Perinatal Period-Part 1: Healthy Foals Born from Normal Pregnancy and Parturition. Vet Sci 2022 Aug 23;9(9).
        doi: 10.3390/vetsci9090451pubmed: 36136667google scholar: lookup
      4. Gibson C, de Ruijter-Villani M, Bauersachs S, Stout TAE. Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy. Int J Mol Sci 2020 Apr 7;21(7).
        doi: 10.3390/ijms21072562pubmed: 32272720google scholar: lookup
      5. Read JE, Cabrera-Sharp V, Offord V, Mirczuk SM, Allen SP, Fowkes RC, de Mestre AM. Dynamic changes in gene expression and signalling during trophoblast development in the horse. Reproduction 2018 Oct 1;156(4):313–330.
        doi: 10.1530/REP-18-0270pubmed: 30306765google scholar: lookup
      6. Read JE, Cabrera-Sharp V, Offord V, Mirczuk SM, Allen SP, Fowkes RC, de Mestre AM. Dynamic changes in gene expression and signalling during trophoblast development in the horse. Reproduction 2018 Oct 1;156(4):313-330.
        doi: 10.1530/REP-18-0270pubmed: 29991567google scholar: lookup
      7. Antczak DF, de Mestre AM, Wilsher S, Allen WR. The equine endometrial cup reaction: a fetomaternal signal of significance. Annu Rev Anim Biosci 2013 Jan;1:419-42.
        doi: 10.1146/annurev-animal-031412-103703pubmed: 25387026google scholar: lookup
      8. Cabrera-Sharp V, Read JE, Richardson S, Kowalski AA, Antczak DF, Cartwright JE, Mukherjee A, de Mestre AM. SMAD1/5 signaling in the early equine placenta regulates trophoblast differentiation and chorionic gonadotropin secretion. Endocrinology 2014 Aug;155(8):3054-64.
        doi: 10.1210/en.2013-2116pubmed: 24848867google scholar: lookup
      9. El-Hashash AH, Warburton D, Kimber SJ. Genes and signals regulating murine trophoblast cell development. Mech Dev 2010 Jan-Feb;127(1-2):1-20.
        doi: 10.1016/j.mod.2009.09.004pubmed: 19755154google scholar: lookup
      10. Mu S, Shen Y, Ren H, Ulaangerel T, Yi M, Zhao B, Hao A, Liu Q, Wen X, Dugarjaviin M, Bou G. Effects of Interleukin-6 (IL-6) on In Vitro Cultured Equine Chorionic Girdle Cells. Animals (Basel) 2025 Feb 6;15(3).
        doi: 10.3390/ani15030450pubmed: 39943220google scholar: lookup
      11. Lessiak U, Melchert M, Walter I, Kummer S, Nell B, Tschulenk W, Pratscher B. Isolation-protocol, characterization, and in-vitro performance of equine umbilical vein endothelial cells. Front Vet Sci 2024;11:1421946.
        doi: 10.3389/fvets.2024.1421946pubmed: 39411390google scholar: lookup
      12. Zeng Z, Li S, Ye X, Wang Y, Wang Q, Chen Z, Wang Z, Zhang J, Wang Q, Chen L, Zhang S, Zou Z, Lin M, Chen X, Zhao G, McAlinden C, Lei H, Zhou X, Huang J. Genome Editing VEGFA Prevents Corneal Neovascularization In Vivo. Adv Sci (Weinh) 2024 Jul;11(25):e2401710.
        doi: 10.1002/advs.202401710pubmed: 38582513google scholar: lookup
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