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Home / Equine Research Bank / Vet Ophthalmol / Immunohistochemical staining of immunoglobulin G in healthy ...
Veterinary ophthalmology2022; 25(3); 232-239; doi: 10.1111/vop.12976

Immunohistochemical staining of immunoglobulin G in healthy equine, canine, and feline corneas.

Abstract: Establishing an immunohistochemical approach for semi-quantitative assessment of the presence of immunoglobulin G (IgG) in equine, canine, and feline corneas. Methods: Healthy corneas of horses, dogs, and cats, euthanized because of a fatal disease or an unrecoverable trauma unrelated to and without a history of ophthalmic disease were formalin-fixed, paraffin-embedded, and determined to be pathomorphologically healthy by light microscopy. Automated immunohistochemistry was performed using primary antibodies against IgG, biotin-conjugated secondary antibodies, and streptavidin-peroxidase, as well as diaminobenzidine for visualization. After counterstaining with hematoxylin, epithelium, stroma, Descemet´s membrane (DM), and endothelium were semi-quantitatively scored for the presence of IgG on a 4-grade scale (0 = no, 1 = faint, 2 = medium, 3 = strong staining) by light microscopy. Results: Corneal specimens of 20 horses (40 eyes) with a median age of 15.5 years (range 2-31 years), 12 dogs (21 eyes) with a median age of 10.0 years (range 4-16), and 13 cats (24 eyes) with a median age of 10.0 years (range 2-18) were included in the study. Different sexes and breeds were represented. In all corneas (100%), significant medium signal intensity in the stroma was observed. Variable immunosignal was obtained in epithelium, DM, and endothelium. Conclusions: This method reproducibly allows for the detection of IgG in healthy equine, canine, and feline corneas, particularly stroma. Semi-quantitative results evidence medium presence of IgG in the corneal stroma. Further research is needed to evaluate IgG presence in diseased corneas.
© 2022 The Authors. Veterinary Ophthalmology published by Wiley Periodicals LLC on behalf of American College of Veterinary Ophthalmologists.
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Publication Date: 2022-03-03 PubMed ID: 35239261PubMed Central: PMC9314887DOI: 10.1111/vop.12976Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article describes a method for detecting the presence of immunoglobulin G (IgG) in the corneas of healthy horses, dogs, and cats. The study found indications of significant medium intensity signals of IgG in the stromal layer of all examined corneas, with varying signals in other parts of the cornea.

Research Methodology

  • The study utilized corneas from horses, dogs, and cats that were euthanized due to fatal diseases or trauma unrelated to eye conditions.
  • The corneas were examined to ensure they were healthy and free of any eye disease.
  • The researchers performed automated immunohistochemistry, which involved using primary antibodies targeting IgG. These antibodies were combined with biotin-conjugated secondary antibodies and streptavidin-peroxidase.
  • The visualization process used diaminobenzidine and counterstaining with hematoxylin.
  • The result was then assessed during light microscopy and scored based on a 4-grade scale for the presence of IgG.

Results

  • The results spanned across 20 horses (40 eyes), 12 dogs (21 eyes), and 13 cats (24 eyes). The age ranges were 2-31 years for horses, 4-16 years for dogs and 2-18 years for cats with differing sexes and breeds represented.
  • All corneas observed (100%) demonstrated a medium signal intensity for IgG specifically within the stroma, while varying levels of immunosignal were observed in other sections such as the epithelium, Descemet’s membrane, and endothelium.

Conclusions

  • The study concluded that the method established allows reliable detection of IgG within healthy corneas of horses, dogs, and cats.
  • The semi-quantitative results demonstrated a medium presence of IgG in the corneal stroma. Further research is recommended to investigate IgG presence in disease-affected corneas.

Cite This Article

APA
Herb VM, Url A, Tichy A, Nell B. (2022). Immunohistochemical staining of immunoglobulin G in healthy equine, canine, and feline corneas. Vet Ophthalmol, 25(3), 232-239. https://doi.org/10.1111/vop.12976

Publication

Veterinary ophthalmology
ISSN: 1463-5224
NlmUniqueID: 100887377
Country: England
Language: English
Volume: 25
Issue: 3
Pages: 232-239

Researcher Affiliations

Herb, Verena Maria
  • Department of Companion Animals and Horses, University of Veterinary Medicine, Vienna, Austria.
Url, Angelika
  • Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Tichy, Alexander
  • Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria.
Nell, Barbara
  • Department of Companion Animals and Horses, University of Veterinary Medicine, Vienna, Austria.

MeSH Terms

  • Animals
  • Cats
  • Cornea / anatomy & histology
  • Dogs
  • Horses
  • Immunoglobulin G
  • Immunohistochemistry
  • Staining and Labeling / veterinary

Conflict of Interest Statement

None.

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