Immunomodulation during and after castration under inhalation anaesthetic without genotoxic effects on equine lymphocytes.
Abstract: Genotoxic DNA damage due to inhalation anaesthesia has been demonstrated in human lymphocytes. In order to evaluate anaesthesia-associated changes in cell-mediated immunity on the basis of a potential DNA damage as a health risk in horses, single cell gel electrophoresis and lymphocyte proliferation assay were performed on equine lymphocytes which were obtained before, during and after regular castration under inhalation anaesthetic. No significant lymphocytic DNA damage due to isoflurane anaesthesia was observed, whereas lymphocyte proliferative reactivity and lymphocyte counts decreased significantly (p≤0.05) during and after anaesthesia. The present study thus indicates that the combined anaesthesia does not result in significant DNA damage, which hence cannot be held responsible for the observed changes in the immune response of equine lymphocytes. However, the recognized compromises of immune function ought to be considered especially in immunologically challenged animals.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication Date: 2011-02-26 PubMed ID: 21354588DOI: 10.1016/j.rvsc.2011.01.021Google Scholar: Lookup
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- Journal Article
Summary
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The research examines the impact of inhalation anesthesia during and after castration on equine (horse) lymphocytes, specifically focusing on DNA damage and changes in cell-mediated immunity. The study concluded that while there was no substantial DNA damage, lymphocyte proliferation and lymphocyte counts significantly decreased during and after anesthesia, indicating an effect on immune function.
Methodology
- The team utilized inhalation anesthesia in the equine subjects during their standard castration procedure for this study.
- They collected equine lymphocytes (a type of white blood cell) at three different stages: before the anesthesia was administered, during the use of anesthesia, and after the use of anesthesia.
- Then, these lymphocytes were further examined using two specific methods: single cell gel electrophoresis (a technique to detect DNA damage) and lymphocyte proliferation assay (a test to measure the ability of lymphocytes to proliferate).
Findings
- No significant lymphocytic DNA damage due to isoflurane anaesthesia (the type of inhalation anesthesia used in this study) was observed.
- However, the tests indicated a significant decrease in lymphocyte counts and lymphocyte proliferative reactivity both during and after anesthesia, pointing to an effect on the immune response of the equine lymphocytes.
Conclusion
- The study, therefore, caused the researchers to assert that the use of combined anesthesia does not result in significant DNA damage.
- However, the observed decreases in lymphocyte counts and proliferation should be taken into account, especially in the case of animals experiencing immune challenges, as these changes may compromise their immune function.
Implications
- These findings add to the body of knowledge on the impacts of anaesthesia on immune function, and can potentially lead to adjustments in anesthesia protocols, particularly for animals with immune system concerns.
- Further research could explore ways to mitigate the decrease in lymphocyte counts and proliferation in response to anesthesia.
Cite This Article
APA
Strasser A, Kühnel H, Velde K, Dadak A.
(2011).
Immunomodulation during and after castration under inhalation anaesthetic without genotoxic effects on equine lymphocytes.
Res Vet Sci, 92(2), 306-310.
https://doi.org/10.1016/j.rvsc.2011.01.021 Publication
Researcher Affiliations
- Institute of Physiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Wien, Austria. alois.strasser@vu-wien.ac.at
MeSH Terms
- Anesthetics, Inhalation / adverse effects
- Anesthetics, Inhalation / pharmacology
- Animals
- Comet Assay / veterinary
- DNA Damage / drug effects
- Horses
- Immunity, Cellular / drug effects
- Ketamine / adverse effects
- Ketamine / pharmacology
- Leukocyte Count / veterinary
- Lymphocytes / drug effects
- Male
- Orchiectomy / methods
- Orchiectomy / veterinary
Citations
This article has been cited 3 times.- Valencak TG, Raith J, Staniek K, Gille L, Strasser A. Lactation Affects Isolated Mitochondria and Its Fatty Acid Composition but Has No Effect on Tissue Protein Oxidation, Lipid Peroxidation or DNA-Damage in Laboratory Mice. Antioxidants (Basel) 2016 Jan 11;5(1).
- Braz MG, Karahalil B. Genotoxicity of Anesthetics Evaluated In Vivo (Animals). Biomed Res Int 2015;2015:280802.
- Strasser A, Carra M, Ghareeb K, Awad W, Böhm J. Protective effects of antioxidants on deoxynivalenol-induced damage in murine lymphoma cells. Mycotoxin Res 2013 Aug;29(3):203-8.
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