Impact of Equine and Bovine Oocyte Maturation in Follicular Fluid From Young and Old Mares on Embryo Production in Vitro.
Abstract: Equine follicular fluid (FF) provides autocrine and paracrine factors from theca, granulosa, and cumulus cells, both reflecting and impacting oocyte and follicle maturation. We hypothesized that maturation of oocytes in FF from old versus young mares has a deleterious effect on oocyte maturation and their subsequent developmental potential. Follicular fluid was collected from the large, dominant follicle from young mares (4-13 years) or old mares (21-26 years) and classified as: (1) Noninduced follicular fluid (NFF), FF from noninduced follicle 33 ± 3 mm, or (2) Induced follicular fluid (IFF), FF collected ∼24 hours after administration of ovulation-inducing drugs when a follicle 33 ± 3 mm was observed. In experiment 1, immature equine oocytes were collected, matured in vitro for 30 ± 2 hours in 100% IFF, collected from young or old mares, with the addition of follicle stimulating hormone (5 mU/mL), then fertilized by intracytoplasmic sperm injection. In experiment 2, immature bovine oocytes were collected, matured in 100% IFF or NFF, collected from young mares or old mares, then fertilized via in vitro fertilization. In experiment 1, more blastocysts tended (P = .08) to be produced from equine oocytes that were matured in old versus young mare FF. In experiment 2, when IFF and NFF groups were combined, cleavage rates were higher (P = .001) when bovine oocytes were matured in FF from young than old mares. In contrast to our hypothesis, we observed no conclusive evidence that FF from old mares has a deleterious impact on oocytes and their early developmental potential.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication Date: 2018-06-22 PubMed ID: 31256896DOI: 10.1016/j.jevs.2018.04.009Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research studied the effect of horse (equine) and cow (bovine) oocyte maturation in the follicular fluid (FF) from young and old horses on in vitro (test tube) production of embryos. The results indicated that the follicular fluid from older mares did not have a negative impact on early development of horse or cow embryos.
Experiment and Hypothesis
- The researchers hypothesized that the process of oocytes maturing in follicular fluid from old horses as compared to young ones might have a negative effect on oocyte maturation and their subsequent developmental potential.
- They conducted two experiments to test this – one using horse oocytes, the other using cow oocytes. Both were matured in follicular fluid from young or old horses and then fertilized.
Types of Follicular Fluid
- Follicular fluid used in these experiments was of two types: Noninduced follicular fluid from noninduced follicles, and Induced follicular fluid collected after administering ovulation-inducing drugs to the mares.
- The noninduced follicular fluid was collected from follicles measuring 33 ± 3 mm, while the induced FF was collected around 24 hours after administering the ovulation-inducing drug, when a follicle of the same size was observed.
Experiment Results
- In the first experiment using horse oocytes, the results tended to show that more embryos (blastocysts) were produced from oocytes matured in the follicular fluid of older mares as compared to the fluid from younger mares.
- In the second experiment with cow oocytes, the oocytes matured in the follicular fluid from younger mares showed higher cleavage rates (a significant step in cell division) when the results of IFF and NFF groups were combined.
- Contrary to their initial hypothesis, the researchers found no conclusive evidence to suggest that follicular fluid from older mares has a negative impact on the oocytes and their early development.
Cite This Article
APA
Spacek SG, Carnevale EM.
(2018).
Impact of Equine and Bovine Oocyte Maturation in Follicular Fluid From Young and Old Mares on Embryo Production in Vitro.
J Equine Vet Sci, 68, 94-100.
https://doi.org/10.1016/j.jevs.2018.04.009 Publication
Researcher Affiliations
- Equine Reproduction Lab, Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO.
- Equine Reproduction Lab, Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. Electronic address: Elaine.Carnevale@colostate.edu.
Citations
This article has been cited 2 times.- Lawson EF, Grupen CG, Baker MA, Aitken RJ, Swegen A, Pollard CL, Gibb Z. Conception and early pregnancy in the mare: lipidomics the unexplored frontier.. Reprod Fertil 2022 Jan 1;3(1):R1-R18.
- Benammar A, Derisoud E, Vialard F, Palmer E, Ayoubi JM, Poulain M, Chavatte-Palmer P. The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?. Animals (Basel) 2021 Aug 4;11(8).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
