Impact of hospitalization and antimicrobial drug administration on antimicrobial susceptibility patterns of commensal Escherichia coli isolated from the feces of horses.
Abstract: Objective-To evaluate antimicrobial susceptibility of commensal Escherichia coli strains isolated from the feces of horses and investigate relationships with hospitalization and antimicrobial drug (AMD) administration. Design-Observational study. Animals-68 hospitalized horses that had been treated with AMDs for at least 3 days (HOSP-AMD group), 63 hospitalized horses that had not received AMDs for at least 4 days (HOSP-NOAMD group), and 85 healthy horses that had not been hospitalized or treated with AMDs (community group). Procedures-Fecal samples were submitted for bacterial culture, and up to 3 E coli colonies were recovered from each sample. Antimicrobial susceptibility of 724 isolates was evaluated. Prevalence of resistance was compared among groups by use of log-linear modeling. Results-For 12 of the 15 AMDs evaluated, prevalence of antimicrobial resistance differed significantly among groups, with prevalence being highest among isolates from the HOSP-AMD group and lowest among isolates from the community group. Isolates recovered from the HOSP-AMD and HOSP-NOAMD groups were also significantly more likely to be resistant to multiple AMDs. Resistance to sulfamethoxazole and resistance to trimethoprim-sulfamethoxazole were most common, followed by resistance to gentamicin and resistance to tetracycline. Use of a potentiated sulfonamide, aminoglycosides, cephalosporins, or metronidazole was positively associated with resistance to 1 or more AMDs, but use of penicillins was not associated with increased risk of resistance to AMDs. Conclusion and Clinical Relevance-Results suggest that both hospitalization and AMD administration were associated with prevalence of antimicrobial resistance among E coli strains isolated from the feces of horses.
Publication Date: 2006-06-21 PubMed ID: 16784384DOI: 10.2460/javma.228.12.1909Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study evaluates the effects of hospitalization and antibiotic drugs on the antibiotic resistance of Escherichia coli (E.coli) strains found in horse feces. Findings indicate a correlation between both hospitalization and drug administration with increased antimicrobial resistance.
Research Design and Animal Subjects
- This is an observational study involving 216 horses divided into three groups. The first group comprised of 68 horses that had been hospitalized and treated with antimicrobial drugs for a minimum of three days, referred to as the HOSP-AMD group.
- The second group consisted of 63 hospitalized horses, not treated with antimicrobial drugs for at least four days, referred to as the HOSP-NOAMD group.
- The third group included 85 healthy horses that had neither been hospitalized nor treated with any antimicrobial drugs, referred to as the community group.
Fecal Sample Procedures
- For all the horses involved in the research, fecal samples were submitted for culturing bacteria.
- From each sample, up to three E.coli colonies were isolated resulting in a total of 724 isolates whose antibiotic susceptibility was subsequently assessed.
Results and Findings
- The study established a significant difference amongst the groups in the prevalence of antimicrobial resistance to 12 out of 15 antimicrobial drugs evaluated.
- The HOSP-AMD group showed the highest prevalence of antimicrobial resistance, while the community group demonstrated the least.
- Isolates from the HOSP-AMD and HOSP-NOAMD groups manifested a higher likelihood of resistance to multiple antimicrobial drugs.
- Resistance to sulfamethoxazole and trimethoprim-sulfamethoxazole was the most common, followed by resistance to gentamicin and tetracycline.
- Use of potentiated sulfonamides, aminoglycosides, cephalosporins, or metronidazole was positively associated with resistance to one or more antimicrobial drugs, while the usage of penicillins was not linked to an increased risk of developing resistance.
Conclusion
- The study suggests a strong association between hospitalization and antibiotic administration with the prevalence of antibiotic resistance among E.coli strains in horse feces.
Cite This Article
APA
Dunowska M, Morley PS, Traub-Dargatz JL, Hyatt DR, Dargatz DA.
(2006).
Impact of hospitalization and antimicrobial drug administration on antimicrobial susceptibility patterns of commensal Escherichia coli isolated from the feces of horses.
J Am Vet Med Assoc, 228(12), 1909-1917.
https://doi.org/10.2460/javma.228.12.1909 Publication
Researcher Affiliations
- Animal Population Health Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
MeSH Terms
- Animals
- Anti-Bacterial Agents / pharmacology
- Anti-Bacterial Agents / therapeutic use
- Cross Infection / veterinary
- Drug Resistance, Bacterial
- Drug Resistance, Multiple, Bacterial
- Escherichia coli / drug effects
- Escherichia coli Infections / drug therapy
- Escherichia coli Infections / microbiology
- Escherichia coli Infections / veterinary
- Feces / microbiology
- Female
- Horse Diseases / drug therapy
- Horse Diseases / microbiology
- Horses
- Hospitalization
- Male
- Microbial Sensitivity Tests / veterinary
Citations
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