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Home / Equine Research Bank / Reprod Domest Anim / Impact of intrauterine infusion of Platelets-Rich plasma on ...
Reproduction in domestic animals = Zuchthygiene2023; 58(5); 622-629; doi: 10.1111/rda.14329

Impact of intrauterine infusion of Platelets-Rich plasma on endometritis and reproductive performance of Arabian mare.

Abstract: Equine endometritis is one of the most common causes of reproduction failure. To achieve better treatment outcomes, different diagnostic methods should be combined. In the current study, 39 repeat breeder mares were subjected to ultrasonography examination to detect excessive accumulation of intrauterine fluids and an abnormal oedema pattern, which revealed that 61.5% of mares were positive. Combined with endometrial cytology by low-volume uterine flush, 47.7% of smears contain neutrophils (more than 2-3 per HPF X100), and microbial culture. 92.3% of mares were infected with different bacterial isolates, such as Escherichia coli, Streptococci, Staphylococcus, Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter freundii, Providencia alcalifaciens, and Proteus mirabilis. All mares were given saline solution and gravity withdrawal before being given 20 IU of oxytocin (as ecbolic agents). Mares were divided into three groups; Group one (n = 15) received an intrauterine infusion of 20 mL of freshly prepared autologous platelet-rich plasma (PRP) 6 h after breeding, Group two (n = 15) was treated with three doses of systemic Enrofloxacin 5% during the estrus period, and Group three (n = 9) received only uterine lavage and 20 IU of oxytocin. PRP and Enrofloxacin resulted in a significant (p < .05) reduction in endometrial thickness (oedema; 5.05 and 6.74 mm, respectively) and disappearance of intrauterine fluids compared to the control (10.98 mm). Furthermore, PRP (days) and Enrofloxacin (17.89 days) reduced the days to the next oestrus compared to the control (18.58 and 17.89 vs. 21.19 days, respectively). Furthermore, the pregnancy rate improved to reach 70% in the PRP group and 60% in the Enrofloxacin group, while the control remained low at 22%. In conclusion, autologous PRP can be used as a low-cost alternative therapy for modulating the inflammatory process and effectively treating mares' endometritis.
© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
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Publication Date: 2023-02-27 PubMed ID: 36807351DOI: 10.1111/rda.14329Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers investigated the effects of intrauterine use of platelet-rich plasma (PRP) on endometritis, a cause of reproductive failure in Arabian mares. They found that this treatment significantly improved endometrial health and reproduction rate in treated mares compared to a control group that did not receive PRP treatment.

Experiment Design

  • In this study, 39 repeat breeder mares underwent ultrasonography to identify accumulation of intrauterine fluids and abnormal oedema patterns associated with endometritis. Nearly 62% of these mares showed positive results for these conditions.
  • The researchers also performed endometrial cytology using a low-volume uterine flush to collect cells for testing. They found that nearly 48% of the samples contained neutrophils, a type of white blood cell involved in inflammation, above the standard level of 2-3 per HPF x100.
  • A microbial culture was also carried out, revealing that over 92% of the mares were infected with different bacterial species known to cause endometritis, including Escherichia coli, Streptococci, and Staphylococcus among others.
  • Before treatment, all mares were given a saline solution and gravity withdrawal, then administered 20 IU of oxytocin, a natural hormone used to control uterine contractions.

Treatment Groups

  • The mares were then divided into three groups for treatment. The first group was treated with an intrauterine infusion of 20 mL of platelet-rich plasma, collected from the mares’ own body, six hours after breeding.
  • The second group was treated with three doses of systemic Enrofloxacin, an antibiotic commonly used against gram-negative bacteria, during the estrus period.
  • The third group served as a control, receiving only uterine lavage (washing out of the uterus) and an additional 20 IU of oxytocin.

Results

  • The treatment with PRP and Enrofloxacin both resulted in a significant reduction in endometrial thickness and disappearance of intrauterine fluids compared to the control group. This indicates a significant reduction in the inflammation caused by endometritis.
  • These treatments also reduced the time to the next oestrus (period of sexual receptivity in female animals), implying a quicker recovery.
  • Most importantly, the pregnancy rates increased significantly: 70% for the PRP group and 60% for the Enrofloxacin group, in contrast to 22% in the control group.

Conclusion

  • The authors concluded that platelet-rich plasma could be a low-cost alternative therapy to modulate the inflammatory process and effectively treat endometritis in mares. While antibiotics like Enrofloxacin also showed promising results, PRP stands out due to its autologous nature (using the animal’s own blood) and easy accessibility.

Cite This Article

APA
Ghallab RS, El-Beskawy M, El-Shereif AA, Rashad AMA, Elbehiry MA. (2023). Impact of intrauterine infusion of Platelets-Rich plasma on endometritis and reproductive performance of Arabian mare. Reprod Domest Anim, 58(5), 622-629. https://doi.org/10.1111/rda.14329

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 58
Issue: 5
Pages: 622-629

Researcher Affiliations

Ghallab, Rezk S
  • Department of Theriogenology, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt.
El-Beskawy, Mohamed
  • Department of animal medicine, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt.
El-Shereif, Abdelghany A
  • Animal and Fish Production Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.
Rashad, Amr M A
  • Animal and Fish Production Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.
Elbehiry, Mohamed A
  • Department of Theriogenology, faculty of veterinary Medicine, Damanhour University, Damanhour, Egypt.

MeSH Terms

  • Pregnancy
  • Horses
  • Animals
  • Female
  • Endometritis / therapy
  • Endometritis / veterinary
  • Endometritis / etiology
  • Oxytocin / pharmacology
  • Blood Platelets
  • Enrofloxacin / pharmacology
  • Reproduction
  • Endometrium / diagnostic imaging
  • Platelet-Rich Plasma
  • Horse Diseases / therapy

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