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Theriogenology2016; 86(2); 516-522; doi: 10.1016/j.theriogenology.2016.01.029

Inflammatory response in chronic degenerative endometritis mares treated with platelet-rich plasma.

Abstract: Degenerative changes of the endometrium are directly related to age and fertility in mares. Chronic degenerative endometritis (CDE) is correlated with uterine fluid retention and reduced ability to clear uterine inflammation. Recent research in the areas of equine surgery and sports medicine has shown that platelet-rich plasma (PRP) treatment acts as an immunomodulator of the inflammatory response. Therefore, the aim of this study was to determine if the uterine infusion of PRP could modulate the local inflammatory response and modify the intrauterine NO concentrations after artificial insemination (AI) in both normal mares and those with CDE. Thirteen mares with endometrium classified as grade III on the histology (mares with CDE) and eight mares with endometrial histological classification I or II-a normal mares were selected to investigate the effect of PRP therapy. The mares were inseminated with fresh semen in two consecutive cycles in a crossover study design. Thereby, each mare served as its own control and the treatment was performed with intrauterine PRP infusion four hours after AI. The percentage of neutrophils in uterine cytology (CIT, %), uterine fluid accumulation observed on ultrasonography (FLU, mm) and nitric oxide concentration of uterine fluid (NO, μM) were analyzed before and 24 hours after AI. The results reported that mares with CDE (CIT, 68.3 ± 3.27, FLU, 10.7 ± 1.61) have a higher (P  0.05) between categories of mares. In treated cycles with PRP, the intrauterine inflammatory response decrease (P < 0.05) in CDE mares (CDE: CIT, 31.4 ± 6.48, FLU, 5.5 ± 1.28; normal mares: CIT, 13.5 ± 4.31, FLU, 0) when compared with nontreated cycle (CDE: CIT, 68.3 ± 3.27, FLU, 10.7 ± 1.61; NM: CIT, 24.4 ± 3.56, FLU, 0), but did not modify NO concentrations in uterine fluid. Thus, we can conclude that PRP was effective in modulating the exacerbated uterine inflammatory response to semen in mares with CDE but did not reduce NO concentrations in intrauterine fluid.
Copyright © 2016 Elsevier Inc. All rights reserved.
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Publication Date: 2016-02-18 PubMed ID: 27020400DOI: 10.1016/j.theriogenology.2016.01.029Google Scholar: Lookup
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Summary

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The study investigates the effect of platelet-rich plasma (PRP) therapy on reducing inflammation and nitric oxide concentrations in mares with Chronic degenerative endometritis (CDE) after artificial insemination (AI). The findings report that PRP effectively reduces inflammation in affected mares but does not modify nitric oxide levels.

Overview of the Research Paper

  • The research paper pertains to a study conducted to explore the effect of PRP therapy on mares with CDE, a condition associated with age and fertility, marked by endometrial degeneration.
  • The major symptoms of CDE include uterine fluid retention and a reduced ability to clear uterine inflammation.
  • The PRP therapy, according to recent studies in equine surgery and sports medicine, acts as an immunomodulator of the inflammatory response.
  • The investigation evaluated how PRP therapy could adjust the local inflammatory response and change intrauterine NO concentrations post-AI in normal mares and those with CDE.

Methodology of the Study

  • The study included thirteen mares with grade III endometrium (indicating mares with CDE) and eight mares with endometrial histological classification I or II (normal mares).
  • Mares were artificially inseminated with fresh semen in two consecutive cycles using a crossover study design, making each mare its own control subject.
  • PRP infusion treatment was executed intrauterine four hours after AI.
  • The analysis involved measuring parameters like percentage of neutrophils in uterine cytology (CIT, %), uterine fluid accumulation observed on ultrasonography (FLU, mm), and nitric oxide concentration of uterine fluid (NO, μM) before and 24 hours after AI.

Results and Conclusion of the Study

  • The study reports higher intrauterine inflammatory response after AI in mares with CDE compared to normal mares.
  • However, the nitric oxide concentrations did not differ considerably between the two mare categories.
  • In treatment cycles involving PRP, the intrauterine inflammatory response noted a decrease in CDE mares.
  • The PRP treatment did not alter nitric oxide concentrations in intrauterine fluid.
  • The study concludes that while PRP therapy was effective in modulating the increased uterine inflammatory response to semen in mares with CDE, it did not succeed in reducing nitric oxide concentrations in intrauterine fluid.

Cite This Article

APA
Reghini MF, Ramires Neto C, Segabinazzi LG, Castro Chaves MM, Dell'Aqua Cde P, Bussiere MC, Dell'Aqua JA, Papa FO, Alvarenga MA. (2016). Inflammatory response in chronic degenerative endometritis mares treated with platelet-rich plasma. Theriogenology, 86(2), 516-522. https://doi.org/10.1016/j.theriogenology.2016.01.029

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 86
Issue: 2
Pages: 516-522

Researcher Affiliations

Reghini, Maria Fernanda S
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Ramires Neto, Carlos
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Segabinazzi, Lorenzo G
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Castro Chaves, Maria Manoela B
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Dell'Aqua, Camila de Paula F
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Bussiere, Maria Clara C
  • Laboratory of Animal Reproduction and Genetic Improvement, North Fluminense State University, Rio de Janeiro, Brazil.
Dell'Aqua, José Antonio
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Papa, Frederico O
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Alvarenga, Marco Antonio
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil. Electronic address: malvarenga@fmvz.unesp.br.

MeSH Terms

  • Animals
  • Case-Control Studies
  • Endometritis / therapy
  • Endometritis / veterinary
  • Female
  • Horse Diseases / therapy
  • Horses
  • Inflammation / metabolism
  • Platelet-Rich Plasma

Citations

This article has been cited 25 times.
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