FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Cryobiology2017; 79; 14-20; doi: 10.1016/j.cryobiol.2017.10.001

Impact of supplementation of semen extender with antioxidants on the quality of chilled or cryopreserved Arabian stallion spermatozoa.

Abstract: The aim of the present study was to evaluate the effects of supplementation of semen extender with various non-enzymatic antioxidants on the quality of cooled or cryopreserved Arabian stallion spermatozoa. Semen collected from four pure Arabian stallions was centrifuged at 600g for 15 min. Spermatozoa were then diluted in INRA-82 extender supplemented with bovine serum albumin (BSA; 0, 10, 15 and 20 mg/mL) or trehalose (0, 75, 100 and 150 mM) or zinc sulphate (0, 100, 150 and 200 μM). The diluted semen was then either cooled at 5 °C or cryopreserved in 0.5-ml plastic straws. After cooling or thawing, sperm motility, viability, sperm abnormalities, viability index, and plasma membrane integrity were evaluated. The results showed that supplementation of semen extender with 150 mM trehalose or with 200 μM zinc sulphate significantly (P < 0.05) improved motility, viability, sperm membrane integrity and acrosome status in Arabian stallion spermatozoa after cooling or after freezing and thawing compared with controls (non-supplemented media) or with those supplemented with other concentrations of trehalose or zinc sulphate. Supplementation of semen extender with BSA did not improve sperm motility or cryosurvival of Arabian stallion spermatozoa after cooling or after freezing and thawing. In conclusion, supplementation of semen extender with non-enzymatic antioxidants (trehalose or zinc sulphate) improved the quality of chilled and frozen/thawed Arabian stallion spermatozoa. The most beneficial effects occur when semen diluent was supplemented with 150 mM trehalose or 200 μM zinc sulphate.
Copyright © 2017 Elsevier Inc. All rights reserved.
Get Full Text
Publication Date: 2017-10-04 PubMed ID: 28986135DOI: 10.1016/j.cryobiol.2017.10.001Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article studies how adding non-enzymatic antioxidants to semen extenders affects the quality of chilled or frozen Arabian stallion sperm. The findings suggest that the addition of certain antioxidants improves the sperm’s health and survival upon cooling or freezing.

Research Context

  • The research is grounded in the understanding of insemination techniques that require the chilling or freezing of sperm (cryopreservation). This process can have deleterious effects on sperm health, affecting fertility.
  • The researchers aimed to mitigate these effects by supplementing the semen extender with non-enzymatic antioxidants.
  • Arabian stallion sperm was used for the experiment, testing the effects of bovine serum albumin (BSA), trehalose, and zinc sulphate at different concentrations.

Research Methodology

  • Semen was collected from four pure Arabian stallions and centrifuged to separate spermatozoa from other components.
  • This spermatozoa were diluted in an INRA-82 extender with varying concentrations of BSA, trehalose, or zinc sulphate.
  • After dilution, the semen was either cooled at 5 °C or cryopreserved in 0.5-ml plastic straws.
  • Upon thawing or cooling, the sperm was evaluated based on motility, viability, sperm abnormalities, viability index, and plasma membrane integrity.

Research Findings

  • The results indicated that supplementing the extender with 150 mM trehalose or 200 μM zinc sulphate significantly improved sperm health and survival after chilling or freezing, over non-supplemented controls or other concentration doses.
  • The improvement was measured in terms of motility, viability, sperm membrane integrity and acrosome status post cooling or thawing. These factors are key to successful fertilization.
  • Adding BSA to the extender, however, did not show any improvement in sperm health or survival after chilling or freezing.

Conclusion

  • The research concludes that non-enzymatic antioxidants, specifically trehalose or zinc sulphate, can enhance the quality of chilled or frozen Arabian stallion sperm.
  • The most effective concentrations were found to be 150 mM for trehalose and 200 μM for zinc sulphate.
  • This could have potential implications for improving breeding success in Arabian stallions using artificial insemination techniques that involve sperm cryopreservation.

Cite This Article

APA
Ghallab AM, Shahat AM, Fadl AM, Ayoub MM, Moawad AR. (2017). Impact of supplementation of semen extender with antioxidants on the quality of chilled or cryopreserved Arabian stallion spermatozoa. Cryobiology, 79, 14-20. https://doi.org/10.1016/j.cryobiol.2017.10.001

Publication

Cryobiology
ISSN: 1090-2392
NlmUniqueID: 0006252
Country: Netherlands
Language: English
Volume: 79
Pages: 14-20

Researcher Affiliations

Ghallab, AbdelRaouf M
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Shahat, Abdallah M
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Fadl, Aya M
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Ayoub, Mohamed M
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Moawad, Adel R
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Department of Surgery (Urology Division), McGill University, Montreal, Canada. Electronic address: adelreda902@hotmail.com.

MeSH Terms

  • Acrosome / drug effects
  • Albumins / pharmacology
  • Animals
  • Antioxidants / pharmacology
  • Cell Membrane / drug effects
  • Cell Membrane / physiology
  • Cryopreservation / methods
  • Cryoprotective Agents / pharmacology
  • Freezing
  • Horses
  • Male
  • Semen / physiology
  • Semen Analysis
  • Semen Preservation / methods
  • Sperm Motility / drug effects
  • Spermatozoa / physiology
  • Trehalose / pharmacology
  • Zinc Sulfate / pharmacology

Citations

This article has been cited 9 times.
  1. Shahat AM, Narlagiri R, Siddique A, Chelkapally SC, Kolikapongu RS, Namani SC, Shaik A, Batchu P, Gurrapu P, Erukulla TT, Neha A, Terrill TH, Moawad AR. Use of Bioelectrical Impedance Analysis to Measure the Impact of Parasitic Infection on Goat Sperm Quality. Animals (Basel) 2025 Dec 17;15(24).
    doi: 10.3390/ani15243624pubmed: 41463910google scholar: lookup
  2. Ullah A, Chen W, Shi L, Wang M, Geng M, Na J, Akhtar MF, Khan MZ, Wang C. Challenges and Enhancing Strategies of Equine Semen Preservation: Nutritional and Genetic Perspectives. Vet Sci 2025 Aug 25;12(9).
    doi: 10.3390/vetsci12090807pubmed: 41012733google scholar: lookup
  3. Ramírez-Chequer JA, Lopez-Carlos MA, Arechiga-Flores CF, Hernandez-Briano P, Medina-Flores CA, Mendez-Llorente F. Effects of cactus (Opuntia ficus-indica) mucilage on boar sperm cryosurvival. Anim Reprod 2025;22(2):e20240004.
    doi: 10.1590/1984-3143-AR2024-0004pubmed: 40599699google scholar: lookup
  4. Li Y, He M, Ran H, Wu J, Lv J, Liu G, Wang Y, Jiang Z. ZnO NPs protect boar sperm in liquid storage through increasing the phosphorylation of PKAs. Anim Reprod 2025;22(2):e20240025.
    doi: 10.1590/1984-3143-AR2024-0025pubmed: 40276358google scholar: lookup
  5. Omidi F, Hajarian H, Karamishabankareh H, Soltani L, Dashtizad M. Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano-NPs), Curcumin Loaded on Zano-NPs on Post-Thawing Quality of Ram Semen. Vet Med Sci 2024 Nov;10(6):e70091.
    doi: 10.1002/vms3.70091pubmed: 39495034google scholar: lookup
  6. Al-Khaldi K, Yimer N, Sadiq MB, Firdaus Jesse Bin Abdullah F, Salam Babji A, Al-Bulushi S. Edible bird's nest supplementation in chilled and cryopreserved Arabian stallion semen. Saudi J Biol Sci 2022 Mar;29(3):1577-1584.
    doi: 10.1016/j.sjbs.2021.11.009pubmed: 35280569google scholar: lookup
  7. Riesco MF, Alvarez M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Boixo JC, de Paz P, Anel L. Multiparametric Study of Antioxidant Effect on Ram Sperm Cryopreservation-From Field Trials to Research Bench. Animals (Basel) 2021 Jan 23;11(2).
    doi: 10.3390/ani11020283pubmed: 33498656google scholar: lookup
  8. Shahin MA, Khalil WA, Saadeldin IM, Swelum AA, El-Harairy MA. Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa. Animals (Basel) 2020 Jan 2;10(1).
    doi: 10.3390/ani10010078pubmed: 31906462google scholar: lookup
  9. Qamar AY, Fang X, Kim MJ, Cho J. Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm. Animals (Basel) 2019 Nov 27;9(12).
    doi: 10.3390/ani9121038pubmed: 31783679google scholar: lookup
Subscribe to our newsletter
Join 99,727 horse owners like you who receive our email updates on horse health and nutrition.