Impaired estrogen production by Leydig cells of the naturally retained testis in unilaterally cryptorchid boars and stallions.
Abstract: Estrogen production in vitro was compared for Leydig cells from cryptorchid and scrotal testes in boars and stallions. Animals with natural and experimental cryptorchidism were used. Purified Leydig cells were prepared from testes of mature animals by collagenase treatment and Percoll density gradients. After incubation for 3 hours (1 X 10(6) cells), estrone sulfate and estrone in the media were measured by direct radioimmunoassay. Androstenedione and testosterone in media extracts also were determined. Cells from the abdominal testis of unilateral cryptorchid boars and stallions showed impaired estrogen production compared with that of the contralateral scrotal testis. Surgical translocation of the scrotal testis to the abdominal cavity in four unilaterally cryptorchid, prepubertal boars did not result in a reduced capacity for estrogen secretion by Leydig cells examined after puberty. Cells from the naturally retained testis in each of these four animals produced practically no estrogen. In a naturally bilateral cryptorchid stallion, there was a high rate of estrogen secretion by both testes. It was concluded that the scrotal testis of a unilaterally cryptorchid animal exerts a suppressive influence on estrogen formation by the abdominal testis.
Publication Date: 1986-03-01 PubMed ID: 2870046DOI: 10.1002/j.1939-4640.1986.tb00888.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research focuses on the comparison of estrogen production in the Leydig cell of cryptorchid (undescended testes) and non-cryptorchid boars and stallions. The study found results indicating that undescended testes had impaired estrogen production when compared to the regular, descended testes.
Research Material and Methodology
- The research utilized boars and stallions with naturally occurring and experimentally induced cryptorchidism.
- Leydig cells, which are crucial for testosterone production, were isolated from the testes of mature animals. This was achieved through collagenase treatment and Percoll density gradients, methods common in cell separation processes.
- Cells were then incubated for 3 hours and the hormones present in the media – estrone sulfate, estrone, androstenedione, and testosterone – were measured using a direct radioimmunoassay, a test that measures the concentration of specific hormones.
Results and Conclusions
- The results showed that the Leydig cells from the undescended testis of unilaterally cryptorchid boars and stallions had impaired estrogen production compared to the Leydig cells from the regular, descended testis.
- The study also performed a surgical translocation wherein the scrotal testis was moved to the abdominal cavity in unilaterally cryptorchid boars. This experiment showed that the translocation did not reduce the ability of the Leydig cells to secrete estrogen after puberty.
- The retained testes in these four animals hardly produced any estrogen. However, in a stallion with bilateral cryptorchidism (both testes undescended), both testes had a high rate of estrogen secretion.
- The conclusion drawn from this research was that the descended testis in a unilaterally cryptorchid animal suppresses estrogen formation in the undescended testis. This potentially points towards the influence of environmental factors (such as temperature) on hormonal production.
Cite This Article
APA
Ryan PL, Friendship RM, Raeside JI.
(1986).
Impaired estrogen production by Leydig cells of the naturally retained testis in unilaterally cryptorchid boars and stallions.
J Androl, 7(2), 100-104.
https://doi.org/10.1002/j.1939-4640.1986.tb00888.x Publication
Researcher Affiliations
MeSH Terms
- Androstenedione / biosynthesis
- Animals
- Cryptorchidism / metabolism
- Cryptorchidism / veterinary
- Estrogens / biosynthesis
- Estrone / analogs & derivatives
- Estrone / biosynthesis
- Horses
- Leydig Cells / metabolism
- Male
- Organ Size
- Swine
- Testis / anatomy & histology
- Testosterone / biosynthesis
Citations
This article has been cited 2 times.- Ouyang Z, Zheng F, Chew JY, Pei Y, Zhou J, Wen K, Han M, Lemieux MJ, Hwang PM, Wen Y. Deciphering the activation and recognition mechanisms of Staphylococcus aureus response regulator ArlR. Nucleic Acids Res 2019 Dec 2;47(21):11418-11429.
- Facemire CF, Gross TS, Guillette LJ Jr. Reproductive impairment in the Florida panther: nature or nurture?. Environ Health Perspect 1995 May;103 Suppl 4(Suppl 4):79-86.
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