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Home / Equine Research Bank / Int J Mol Sci / Implication of Cellular Senescence in Osteoarthritis: A Stud...
International journal of molecular sciences2023; 24(4); doi: 10.3390/ijms24043109

Implication of Cellular Senescence in Osteoarthritis: A Study on Equine Synovial Fluid Mesenchymal Stromal Cells.

Abstract: Osteoarthritis (OA) is described as a chronic degenerative disease characterized by the loss of articular cartilage. Senescence is a natural cellular response to stressors. Beneficial in certain conditions, the accumulation of senescent cells has been implicated in the pathophysiology of many diseases associated with aging. Recently, it has been demonstrated that mesenchymal stem/stromal cells isolated from OA patients contain many senescent cells that inhibit cartilage regeneration. However, the link between cellular senescence in MSCs and OA progression is still debated. In this study, we aim to characterize and compare synovial fluid MSCs (sf-MSCs), isolated from OA joints, with healthy sf-MSCs, investigating the senescence hallmarks and how this state could affect cartilage repair. Sf-MSCs were isolated from tibiotarsal joints of healthy and diseased horses with an established diagnosis of OA with an age ranging from 8 to 14 years. Cells were cultured in vitro and characterized for cell proliferation assay, cell cycle analysis, ROS detection assay, ultrastructure analysis, and the expression of senescent markers. To evaluate the influence of senescence on chondrogenic differentiation, OA sf-MSCs were stimulated in vitro for up to 21 days with chondrogenic factors, and the expression of chondrogenic markers was compared with healthy sf-MSCs. Our findings demonstrated the presence of senescent sf-MSCs in OA joints with impaired chondrogenic differentiation abilities, which could have a potential influence on OA progression.
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Publication Date: 2023-02-04 PubMed ID: 36834521PubMed Central: PMC9967174DOI: 10.3390/ijms24043109Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on studying the role of cell aging (senescence) in osteoarthritis, a degenerative joint disease, using horse joint fluid samples. The study particularly examines how senescence in mesenchymal stem cells isolated from synovial fluid (joint fluid) could contribute to osteoarthritis progression and affect joint repair.

Research Purpose

  • The main goal of this study is to explore the potential links between cellular senescence in mesenchymal stem cells and the progression of osteoarthritis.
  • More specifically, the researchers aim to characterize and compare mesenchymal stem cells from diseased (osteoarthritic) horse joints to those from healthy joints, to investigate signs of senescence and how this might impact the natural repair processes of joint cartilage.

Methodology

  • The researchers isolated mesenchymal stem cells from synovial fluid (joint fluid) from both healthy and osteoarthritic joints of horses aged 8 to 14 years.
  • These cells were then cultured in a lab setting and analyzed through a range of methods including cell proliferation assays, cell cycle analyses, reactive oxygen species (ROS) detection assays, ultrastructure analyses, and the examination of aging-related markers.

Investigation of Senescence Effect on Chondrogenic Differentiation

  • Chondrogenic differentiation is the process by which stem cells transform into chondrocytes; cells that produce and maintain the cartilaginous matrix in joints. This process is crucial for cartilage repair.
  • To investigate the possible influence of senescence on this process, the researchers initiated chondrogenic differentiation in lab-cultured mesenchymal stem cells from osteoarthritic joints by exposing them to chondrogenic factors for 21 days.
  • The expression of markers indicative of chondrogenic differentiation in these cells was then compared to expression in healthy cells.

Findings

  • The main finding of this study was the identification of senescent mesenchymal stem cells in osteoarthritic joints.
  • The researchers found that these cells exhibited impaired chondrogenic differentiation abilities, indicating that cell senescence could potentially affect the natural cartilage repair process and contribute to the progression of osteoarthritis.

Cite This Article

APA
Teti G, Mazzotti E, Gatta V, Chiarini F, Alfieri ML, Falconi M. (2023). Implication of Cellular Senescence in Osteoarthritis: A Study on Equine Synovial Fluid Mesenchymal Stromal Cells. Int J Mol Sci, 24(4). https://doi.org/10.3390/ijms24043109

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 24
Issue: 4

Researcher Affiliations

Teti, Gabriella
  • Department of Biomedical and Neuromotor Sciences, University di Bologna, 40126 Bologna, Italy.
Mazzotti, Eleonora
  • Boehringer Ingelheim Vetmedica GmbH, 55218 Ingelheim am Rhein, Germany.
Gatta, Valentina
  • Department of Biomedical and Neuromotor Sciences, University di Bologna, 40126 Bologna, Italy.
Chiarini, Francesca
  • Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy.
Alfieri, Maria Laura
  • Department of Biomedical and Neuromotor Sciences, University di Bologna, 40126 Bologna, Italy.
Falconi, Mirella
  • Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, Italy.

MeSH Terms

  • Horses
  • Animals
  • Synovial Fluid
  • Cells, Cultured
  • Osteoarthritis / metabolism
  • Cellular Senescence / physiology
  • Cell Differentiation
  • Mesenchymal Stem Cells / metabolism
  • Chondrogenesis

Conflict of Interest Statement

The authors declare no conflict of interest.

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