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The new microbiologica2021; 44(1); 19-23;

Importance of broth-enrichment culture in equine endometritis diagnosis.

Abstract: The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and Streptococcus equi subsp. zooepidemicus (15.2%), both frequently associated with equine endometritis. Our results indicate that the addition enrichment-broth culture significantly increases the rate of positivity for the detection of bacteria in equine uterine swabs compared to the direct plating of samples alone. Thus, this diagnostic technique may be recommended to increase the sensitivity of bacteriological analysis in mares with endometritis.
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Publication Date: 2021-01-14 PubMed ID: 33582824
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the efficiency of using a broth-enrichment culture step in identifying bacterial endometritis in horses. The results demonstrated an increased rate of detecting bacteria in the uterus’ swabs when samples go through a broth-enrichment phase before plating, revealing its potential importance for accurate diagnosis.

Objective and Methodology

  • The study aimed to assess the diagnostic accuracy in identifying bacterial endometitis (an inflammation of the inner layer of the uterus) in horses using standard microbiological techniques and additional broth-enrichment culture.
  • During the research, 452 equine uterine swabs were cultured on different agar plates as well as in a broth-enrichment medium (Brain Heart Infusion Broth) before additional plating.

Findings

  • The results showed a significant increase in the detection of bacteria following the use of added enrichment-broth phase in comparison to direct plating of samples. The positivity rate almost doubled from 33.7% in direct plating to 66.3% when using the enrichment-broth stage.
  • The research also indicated the prevalence of certain bacteria, including E. coli (29.7% ) and Streptococcus equi subsp. zooepidemicus (15.2%), both often linked with equine endometritis.

Conclusion and Recommendation

  • The study concluded that adding an enrichment-broth culture phase could significantly improve the detection rate of bacteria in uterine swabs, indicating its importance for accurate diagnosis.
  • The researchers recommend using this method to increase the sensitivity of bacteriological analysis when diagnosing endometritis in mares. This enhanced technique could potentially increase the accuracy and efficiency of identifying bacterial endometritis in equines, aiding prompt treatment and recovery.

Cite This Article

APA
(2021). Importance of broth-enrichment culture in equine endometritis diagnosis. New Microbiol, 44(1), 19-23.

Publication

The new microbiologica
ISSN: 1121-7138
NlmUniqueID: 9516291
Country: Italy
Language: English
Volume: 44
Issue: 1
Pages: 19-23

Researcher Affiliations

MeSH Terms

  • Animals
  • Culture Media
  • Endometritis / diagnosis
  • Endometritis / veterinary
  • Escherichia coli
  • Female
  • Horse Diseases / diagnosis
  • Horses
  • Humans
  • Streptococcus equi

Citations

This article has been cited 12 times.
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    doi: 10.3390/antibiotics11010025pubmed: 35052902google scholar: lookup
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  9. Del Prete C, Attolini E, Merlo B, Iacono E, Nocera FP, De Martino L, Longobardi C, Damiano S, Longobardi V, Cocchia N, Pasolini MP. Post-Insemination Infusion of Wharton's Jelly Mesenchymal Stromal/Stem Cells-Derived Conditioned Medium: A Novel Approach for Improving Pregnancy Outcomes in Problem Mares. Vet Sci 2025 May 16;12(5).
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  10. Jimenez Rihuete P, Martin C, Villarino NF, Rubio-Martínez LM. Effect of silica-sprayed collection tubes on synovial fluid bacterial culture. Vet Med Sci 2024 Jul;10(4):e1501.
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    doi: 10.1016/j.heliyon.2024.e24994pubmed: 38318038google scholar: lookup
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