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Ticks and tick-borne diseases2019; 11(2); 101329; doi: 10.1016/j.ttbdis.2019.101329

Importance of equine piroplasmosis antibody presence in Spanish horses prior to export.

Abstract: Serological analysis of equine piroplasmosis (EP), caused by Theileria equi and Babesia caballi, is included in the export testing requirements for most of the countries worldwide, thus involving a high economic impact on equine industry of EP-endemic countries, such as Spain. A total of 3368 serum samples from healthy horses collected prior to export between 2015 and 2018 in Spain were tested for antibodies against T. equi and B. caballi by using a competitive inhibition enzyme-linked immunosorbent assay (cELISA). The overall seroprevalence results in Spain revealed that almost a quarter of the horses analysed (24.1 %; 95% CI 22.6-25.5) could not be exported to countries free from EP. The implementation of prevention measures such as the use of acaricides and daily checks for ticks in horses, as well as regular serological screening of horses in Spain would aid to increase the number of horses exported to other countries.
Publication Date: 2019-11-08 PubMed ID: 31734215DOI: 10.1016/j.ttbdis.2019.101329Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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The research article investigates the prevalence of equine piroplasmosis (EP), a disease caused by parasites, in Spanish horses set for export between 2015 and 2018. The study highlights the economic implications of EP prevalence on the equine industry, and suggests preventive measures that would allow a higher number of horses to be exported.

Research Objective and Background

  • The study aimed to analyze the presence of antibodies against equine piroplasmosis (EP), a parasitic disease in horses caused by Theileria equi and Babesia caballi, in Spanish horses set for export.
  • EP plays a significant role in export testing requirements for horses globally, and the presence of EP can impact the economic viability of horse exports, especially for EP-endemic countries like Spain.
  • The researchers used serological tests (blood tests that look for antibodies) to determine the prevalence of EP in Spanish horses.

Methodology and Findings

  • The researchers tested 3368 serum samples from healthy horses collected prior to export between 2015 and 2018.
  • A competitive inhibition enzyme-linked immunosorbent assay (cELISA), a technique used to measure the amount of antibodies in a solution, was used to detect antibodies against T. equi and B. caballi.
  • The study found that about 24.1% of the horses tested had antibodies against EP, indicating they were infected with the disease and thus couldn’t be exported to EP-free countries.

Implications and Recommendations

  • The high prevalence of EP in Spanish horses intended for export poses significant economic implications, as a quarter of the horses analysed couldn’t be exported due to the disease.
  • To combat this issue, the researchers suggest implementing preventive measures like using acaricides (pesticides that kill ticks and mites) and checking horses for ticks daily.
  • Additionally, regular serological screening of horses would help detect and manage the disease, thereby potentially increasing the number of horses that can be exported from Spain.

Cite This Article

APA
Camino E, Pozo P, Dorrego A, Carvajal KA, Buendia A, Gonzalez S, de Juan L, Dominguez L, Cruz-Lopez F. (2019). Importance of equine piroplasmosis antibody presence in Spanish horses prior to export. Ticks Tick Borne Dis, 11(2), 101329. https://doi.org/10.1016/j.ttbdis.2019.101329

Publication

ISSN: 1877-9603
NlmUniqueID: 101522599
Country: Netherlands
Language: English
Volume: 11
Issue: 2
Pages: 101329
PII: S1877-959X(19)30123-2

Researcher Affiliations

Camino, Eliazar
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Pozo, Pilar
  • MAEVA SERVET, S.L., Alameda del Valle, Spain.
Dorrego, Abel
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain.
Carvajal, Kelly Alejandra
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain.
Buendia, Aranzazu
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain.
Gonzalez, Sergio
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain.
de Juan, Lucia
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Dominguez, Lucas
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Cruz-Lopez, Fatima
  • VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain. Electronic address: fatimacr@ucm.es.

MeSH Terms

  • Animals
  • Antibodies, Protozoan / blood
  • Babesiosis / economics
  • Babesiosis / epidemiology
  • Cross-Sectional Studies
  • Female
  • Horse Diseases / economics
  • Horse Diseases / epidemiology
  • Horses
  • Male
  • Prevalence
  • Seroepidemiologic Studies
  • Spain

Conflict of Interest Statement

Declaration of Competing Interest The authors declare no conflict of interest in the subject, matter or materials discussed in this manuscript.

Citations

This article has been cited 4 times.
  1. Bravo-Barriga D, Serrano-Aguilera FJ, Barrasa-Rita R, Habela MÁ, Chacón RB, Ezquerra LJ, Martín-Cuervo M. Effects of Competitive ELISA-Positive Results of Piroplasmosis on the Performance of Endurance Horses.. Animals (Basel) 2022 Mar 3;12(5).
    doi: 10.3390/ani12050637pubmed: 35268210google scholar: lookup
  2. Nadal C, Marsot M, Le Metayer G, Boireau P, Guillot J, Bonnet SI. Spatial and Temporal Circulation of Babesia caballi and Theileria equi in France Based on Seven Years of Serological Data.. Pathogens 2022 Feb 9;11(2).
    doi: 10.3390/pathogens11020227pubmed: 35215171google scholar: lookup
  3. Sears K, Knowles D, Dinkel K, Mshelia PW, Onzere C, Silva M, Fry L. Imidocarb Dipropionate Lacks Efficacy against Theileria haneyi and Fails to Consistently Clear Theileria equi in Horses Co-Infected with T. haneyi.. Pathogens 2020 Dec 10;9(12).
    doi: 10.3390/pathogens9121035pubmed: 33321715google scholar: lookup
  4. Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny.. Pathogens 2020 Nov 8;9(11).
    doi: 10.3390/pathogens9110926pubmed: 33171698google scholar: lookup