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Home / Equine Research Bank / Equine Vet J / In vitro analysis of equine, bone marrow-derived mesenchymal...
Equine veterinary journal2013; 46(5); 589-595; doi: 10.1111/evj.12142

In vitro analysis of equine, bone marrow-derived mesenchymal stem cells demonstrates differences within age- and gender-matched horses.

Abstract: Stem cell therapies are used routinely in equine practice. Most published reports characterise stem cells derived from younger horses; however, middle-aged horses are often in athletic performance, and experience degenerative medical conditions. Thus, mesenchymal stem cells (MSCs) from this group should be investigated. Objective: To describe differences in in vitro adherence, proliferation and potential for differentiation of equine bone marrow-derived MSCs (equine BMMSCs) harvested from middle-aged (10-13 years old) female donors. Methods: Descriptive study of stem cell characteristics. Methods: Equine BMMSCs from 6 horses were cultured in vitro and evaluated for viability, proliferation, osteogenesis, chondrogenesis, adipogenesis, cluster-of-differentiation markers and gene expression. Results: Equine BMMSCs from all 6 donors demonstrated fibroblastic, cellular morphology, adherence to plastic and expression of cluster-of-differentiation markers. They varied in their rate of proliferation and trilineage differentiation. The equine BMMSCs of one of 6 donors demonstrated a higher rate of proliferation, enhanced ability for cell passaging and a more robust in vitro differentiation. Comparatively, equine BMMSCs from 2 donors demonstrated a lower rate of proliferation and lack of osteogenic and chondrogenic differentiation. Conclusions: The results of this study confirm that donor-to-donor variation in equine BMMSCs exists and this variation can be documented using in vitro assays. Subjective assessment suggests that the rate of proliferation tends to correlate with differentiation potential.
© 2013 EVJ Ltd.
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Publication Date: 2013-12-17 PubMed ID: 23855680DOI: 10.1111/evj.12142Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examined the differences in the characteristics and behaviour of stem cells derived from middle-aged horses’ bone marrow. It found notable variations among donors in aspects such as cell proliferation and the potential for differentiation into specific types of cells.

Research Background and Objective

  • The study was prompted by the common use of stem cell therapies in equine practice, typically using cells from younger horses. However, middle-aged horses often continue to have athletic use and develop degenerative medical conditions, warranting investigation into the potential of their stem cells.
  • The main objective was to observe any differences among stem cells from middle-aged (10-13 years old) female horses, in terms of their ability to stick to surfaces (adherence), multiply (proliferation), and change into different cell types (differentiation).

Methods

  • The researchers conducted an in-depth study of the stem cells’ features, cultivating them in a laboratory setting (in vitro).
  • Bone marrow-derived mesenchymal stem cells (BMMSCs) were collected from 6 horses and examined for several criteria, including cell survival, multiplication, potential for differentiation into bone, cartilage and fat cells, presence of specific surface markers, and gene expression.

Results

  • All the harvested stem cells showed the expected properties of fibroblastic cell shape, the ability to stick to plastic, and characteristically expressed markers.
  • Notably, there were appreciable differences in the rate of cell multiplication and the capacity for differentiation into three cell types. One of the six horse donors’ stem cells showed a high rate of multiplication, better potential for maintaining a cell line, and robust ability to differentiate in the laboratory setting.
  • In contrast, stem cells from two other donors showed a lower multiplication rate and failed to differentiate into bone and cartidge cells.

Conclusions

  • The findings confirmed the existence of donor-to-donor variation in horse BMMSCs, as highlighted by the in vitro assays used in the study.
  • The researchers also observed a tendency for higher rates of cell multiplication to go hand-in-hand with better potential for differentiation. This implies that more robust cell proliferation might signal a greater therapeutic potential of stem cells from certain donors.

Cite This Article

APA
Carter-Arnold JL, Neilsen NL, Amelse LL, Odoi A, Dhar MS. (2013). In vitro analysis of equine, bone marrow-derived mesenchymal stem cells demonstrates differences within age- and gender-matched horses. Equine Vet J, 46(5), 589-595. https://doi.org/10.1111/evj.12142

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 46
Issue: 5
Pages: 589-595

Researcher Affiliations

Carter-Arnold, J L
  • Department of Large Animal Clinical Sciences, University of Tennessee, USA.
Neilsen, N L
    Amelse, L L
      Odoi, A
        Dhar, M S

          MeSH Terms

          • Animals
          • Biomarkers
          • Bone Marrow Cells / cytology
          • Bone Marrow Cells / physiology
          • Cell Differentiation
          • Cell Proliferation
          • Cell Survival
          • Cells, Cultured
          • Female
          • Gene Expression Regulation / physiology
          • Horses / physiology
          • Mesenchymal Stem Cells / cytology
          • Mesenchymal Stem Cells / physiology

          Citations

          This article has been cited 16 times.
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