In Vitro Antimicrobial Activity of Selected Essential Oils Against Endometritis-Causing Microorganisms in Mares.
Abstract: This study aimed to evaluate the in vitro antimicrobial activity of essential oils (EO) from Ocimum basilicum (basil), Rosmarinus officinalis (rosemary), and Cymbopogon citratus (lemongrass) on endometritis-causing microorganisms in mares. Serial concentrations of the EO from 30.00 mg/mL to 0.47 mg/mL were tested. The major compounds of O. basilicum EO were linalyl acetate (33.32 wt.%) and citronellal (25.06 wt.%); of R. officinalis EO were borneol (26.48 wt.%), trans-β-ocimene (16.76 wt.%), camphene (12.45 wt.%), and α-phellandrene (11.08 wt.%); and of C. citratus EO were geranial (45.96 wt.%) and neral (32.62 wt.%). Regarding antimicrobial activity, C. citratus EO has had the highest inhibition percentage (73.9%), followed by O. basilicum (67.2%) and R. officinalis (58.7%). P. aeruginosa was the only pathogen unable to establish the minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) values for the studied EO. The EOs were effective against all other microorganisms (S. equi, S. aureus, K. pneumoniae, E. coli, and C. Albicans). In conclusion, the EOs of O. basilicum, R. officinalis, and C. citratus have presented in vitro antimicrobial activity against microorganisms causing endometritis in mares.
Copyright © 2021. Published by Elsevier Inc.
Publication Date: 2021-12-16 PubMed ID: 34923074DOI: 10.1016/j.jevs.2021.103840Google Scholar: Lookup
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Summary
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The research focuses on investigating the potential antimicrobial effects of essential oils derived from basil, rosemary, and lemongrass on bacteria causing endometritis in horses. The study revealed that all three oils exhibited antimicrobial activity, with the lemongrass oil showing the highest effectiveness.
Study Purpose and Methodology
- The primary objective of the research was to assess the antimicrobial properties of essential oils from Ocimum basilicum (basil), Rosmarinus officinalis (rosemary), and Cymbopogon citratus (lemongrass) against bacteria leading to endometritis in mares.
- The analysis involved utilizing different concentrations of the essential oils ranging from 30.00 mg/mL to 0.47 mg/mL.
- The study also identified the major compounds present in each type of essential oil.
Result Summary
- In terms of the principal compounds, the essential oil of basil was found to contain linalyl acetate and citronellal, rosemary oil consisted of borneol, trans-β-ocimene, camphene, and α-phellandrene, while lemongrass oil was primarily composed of geranial and neral.
- The study assessed the antimicrobial effectivity of the essential oils, with the highest inhibition observed in the case of lemongrass oil at 73.9%. Basil and rosemary oils also exhibited substantial antimicrobial activity with percentages of 67.2% and 58.7% respectively.
- Among the tested pathogens, P. aeruginosa was the only type where the minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) regarding the essential oils couldn’t be determined.
- However, all the essential oils emerged as effective against all other types of microorganisms involved in the study which included S. equi, S. aureus, K. pneumoniae, E. coli, and C. Albicans.
Implications and Conclusion
- The implications of the results indicate the potential for using essential oils as antimicrobial solutions in managing endometritis in horses. This could be particularly significant since the oils demonstrated substantial antimicrobial activity against a range of bacteria.
- In conclusion, the study substantiates the in vitro antimicrobial properties of essential oils derived from basil, rosemary, and lemongrass against endometritis causing microorganisms in horses.
Cite This Article
APA
Lisboa FP, Silvestre WP, Castro JO, Martins GV, Segabinazzi LGTM, Pauletti GF, Dell'Aqua JA.
(2021).
In Vitro Antimicrobial Activity of Selected Essential Oils Against Endometritis-Causing Microorganisms in Mares.
J Equine Vet Sci, 110, 103840.
https://doi.org/10.1016/j.jevs.2021.103840 Publication
Researcher Affiliations
- Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University - UNESP, Botucatu, Brazil; Laboratory of Studies of the Soil, Plant, and Atmosphere System and Plant Metabolism, University of Caxias do Sul, Caxias do Sul, Brazil. Electronic address: pxlisboa@gmail.com.
- Laboratory of Studies of the Soil, Plant, and Atmosphere System and Plant Metabolism, University of Caxias do Sul, Caxias do Sul, Brazil.
- Postgraduate Program in Biotechnology, University of Caxias do Sul, Caxias do Sul, Brazil.
- Postgraduate Program in Biotechnology, University of Caxias do Sul, Caxias do Sul, Brazil.
- Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University - UNESP, Botucatu, Brazil; Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies.
- Laboratory of Studies of the Soil, Plant, and Atmosphere System and Plant Metabolism, University of Caxias do Sul, Caxias do Sul, Brazil; Postgraduate Program in Biotechnology, University of Caxias do Sul, Caxias do Sul, Brazil.
- Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University - UNESP, Botucatu, Brazil.
MeSH Terms
- Animals
- Anti-Infective Agents / pharmacology
- Endometritis / drug therapy
- Endometritis / veterinary
- Escherichia coli
- Female
- Horse Diseases
- Horses
- Oils, Volatile / pharmacology
- Staphylococcus aureus
Citations
This article has been cited 5 times.- Mazzuchini MP, Lisboa FP, de Castro JI, Alvarenga MA, Segabinazzi LGTM, Canisso IF. In vitro antimicrobial activity of non-traditional therapies for infectious endometritis in mares. Equine Vet J 2025 Jul;57(4):1118-1126.
- Saeed SI, Kamaruzzaman NF, Gahamanyi N, Nguyen TTH, Hossain D, Kahwa I. Confronting the complexities of antimicrobial management for Staphyloccous aureus causing bovine mastitis: an innovative paradigm. Ir Vet J 2024 Feb 28;77(1):4.
- Silva JA, Castañares M, Mouguelar H, Valenciano JA, Pellegrino MS. Isolation of lactic acid bacteria from the reproductive tract of mares as potentially beneficial strains to prevent equine endometritis. Vet Res Commun 2024 Jun;48(3):1353-1366.
- Luu-Dam NA, Le CVC, Satyal P, Le TMH, Bui VH, Vo VH, Ngo GH, Bui TC, Nguyen HH, Setzer WN. Chemistry and Bioactivity of Croton Essential Oils: Literature Survey and Croton hirtus from Vietnam. Molecules 2023 Mar 3;28(5).
- Radice M, Durofil A, Buzzi R, Baldini E, Martínez AP, Scalvenzi L, Manfredini S. Alpha-Phellandrene and Alpha-Phellandrene-Rich Essential Oils: A Systematic Review of Biological Activities, Pharmaceutical and Food Applications. Life (Basel) 2022 Oct 14;12(10).
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