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Journal of global antimicrobial resistance2019; 19; 144-153; doi: 10.1016/j.jgar.2019.03.006

In vitro antimicrobial susceptibility of equine clinical isolates from France, 2006-2016.

Abstract: This study aimed to analyse antimicrobial susceptibility evolution of equine pathogens isolated from clinical samples from 2006-2016. A collection of 25 813 bacterial isolates was studied, clustered according to their origins (respiratory tract, cutaneous, genital and other), and analysed for their antimicrobial susceptibility using the disk diffusion method. The most frequently isolated pathogens were group C Streptococci (27.6%), Escherichia coli (20.0%), Staphylococcus aureus (7.8%), Pseudomonas aeruginosa (4.0%), Enterobacter spp. (3.4%), Klebsiella pneumoniae (2.4%), and Rhodococcus equi (1.8%). Of the isolates, 9512 were from respiratory samples (36.8%), 7689 from genital origin (29.8%), and 4083 from cutaneous samples (15.8%). Over the 11-year period, the frequency of multidrug-resistant (MDR) strains fluctuated between 6.4-20.4% for group C Streptococci and 17-37.7% for Klebsiella pneumoniae. From 2006-2009, 24.5-43.0% of Staphylococcus aureus isolates were MDR; after 2009 the level did not exceeded 27.6%. For Escherichia coli and Enterobacter spp., these levels were mostly >30.0% until 2012, but significantly decreased thereafter (22.5-26.3%). This study is the first large-scale analysis of equine pathogens, by the number of samples and duration of study. The results showed high levels of MDR strains and the need to support veterinary antimicrobial stewardship to encourage proper use of antibiotics.
Copyright © 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved.
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Publication Date: 2019-03-15 PubMed ID: 30880244DOI: 10.1016/j.jgar.2019.03.006Google Scholar: Lookup
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Summary

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The research article presents a large-scale analysis of the evolution of antimicrobial susceptibility in equine pathogens isolated from clinical samples over 11 years. It highlights the frequency of multi-drug resistant strains and emphasizes the importance of responsible use of antibiotics in veterinary medicine.

Objective and Methodology

  • The main aim of this research was to study the antimicrobial susceptibility evolution in various pathogens affecting horses, isolated from clinical samples over a period of 11 years (from 2006 to 2016).
  • The study analyzed a large collection of 25,813 bacterial isolates. These isolates were grouped according to their origin: respiratory tract, cutaneous (skin), genital, and others.
  • The antimicrobial susceptibility of these isolates was then examined using the disk diffusion method, a technique used to assess the effectiveness of antibiotics against bacteria.

Main Findings

  • The most common pathogens isolated were group C Streptococci (27.6%), Escherichia coli (20.0%), Staphylococcus aureus (7.8%), Pseudomonas aeruginosa (4.0%), Enterobacter spp. (3.4%), Klebsiella pneumoniae (2.4%), and Rhodococcus equi (1.8%).
  • Respiratory samples yielded the highest number of isolates—9512, or 36.8% of the total. These were followed by genital samples (29.8%) and skin samples (15.8%).
  • The frequency of multi-drug resistant (MDR) strains fluctuated over the study period. Particularly, it ranged between 6.4-20.4% for group C Streptococci and 17.0-37.7% for Klebsiella pneumoniae.
  • For Staphylococcus aureus, between 2006 and 2009, 24.5-43.0% were MDR; after 2009, the percentage did not exceeded 27.6%.
  • Escherichia coli and Enterobacter spp. exhibited MDR levels of more than 30.0% until 2012, after which there was a significant decrease, with levels ranging between 22.5-26.3%.

Significance of the Study

  • This study is significant as it represents the first large-scale analysis of its kind, given the number of samples and the duration of the study.
  • The findings of the study show high levels of multi-drug resistant strains amongst equine pathogens. This points to the urgent necessity for responsible use of antibiotics in veterinary practices, also known as antimicrobial stewardship.

Cite This Article

APA
Duchesne R, Castagnet S, Maillard K, Petry S, Cattoir V, Giard JC, Leon A. (2019). In vitro antimicrobial susceptibility of equine clinical isolates from France, 2006-2016. J Glob Antimicrob Resist, 19, 144-153. https://doi.org/10.1016/j.jgar.2019.03.006

Publication

Journal of global antimicrobial resistance
ISSN: 2213-7173
NlmUniqueID: 101622459
Country: Netherlands
Language: English
Volume: 19
Pages: 144-153

Researcher Affiliations

Duchesne, Rachel
  • LABÉO Frank Duncombe, 14053 Caen, France; Normandie Univ, UNICAEN, U2RM, 14033 Caen, France.
Castagnet, Sophie
  • LABÉO Frank Duncombe, 14053 Caen, France; Normandie Univ, UNICAEN, U2RM, 14033 Caen, France.
Maillard, Karine
  • LABÉO Frank Duncombe, 14053 Caen, France.
Petry, Sandrine
  • ANSES, Dozulé Laboratory for Equine Diseases, Bacteriology Unit, 14430 Goustranville, France.
Cattoir, Vincent
  • University Hospital of Rennes, Department of Clinical Microbiology, 35033 Rennes, France; National Reference Center for Antimicrobial Resistance (lab Enterococci), 35033 Rennes, France.
Giard, Jean-Christophe
  • Normandie Univ, UNICAEN, U2RM, 14033 Caen, France.
Leon, Albertine
  • LABÉO Frank Duncombe, 14053 Caen, France; Normandie Univ, UNICAEN, U2RM, 14033 Caen, France. Electronic address: albertine.leon@laboratoire-labeo.fr.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Bacteria / drug effects
  • Bacteria / isolation & purification
  • Bacterial Infections / veterinary
  • Disk Diffusion Antimicrobial Tests
  • Drug Resistance, Multiple, Bacterial
  • Enterobacter / drug effects
  • Enterobacter / isolation & purification
  • Escherichia coli / drug effects
  • France
  • Horse Diseases / microbiology
  • Horses
  • Klebsiella pneumoniae / drug effects
  • Pseudomonas aeruginosa / drug effects
  • Rhodococcus equi / drug effects
  • Rhodococcus equi / isolation & purification
  • Staphylococcus aureus / drug effects
  • Streptococcus / drug effects
  • Streptococcus / isolation & purification

Citations

This article has been cited 15 times.
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