In vitro assessment of two species of nematophagous fungi (Arthrobotrys oligospora and Arthrobotrys flagrans) to control the development of infective cyathostome larvae from naturally infected horses.
Abstract: The ability of two species of nematophagous fungi, Arthrobotrys oligospora and Arthrobotrys flagrans (syn. Trichothecium flagrans, Duddingtonia flagrans), to control the development of infective larvae in feces from naturally infected horses was assessed in vitro. The horses were from a farm where it had been previously established that cyathostomes accounted for 100% of the strongyle egg output. The feces from these naturally infected horses were mixed with spores of each fungal species at four concentrations: 0 (control), 1, 10, and 100 spores per egg. Five replicates for each group were incubated for 8 days. Infective larvae were harvested using a Baermann technique and counted. The percentage reduction in infective cyathostome larvae was calculated for each fungal concentration compared to controls. A fungal concentration of 1 spore per egg resulted in 40.5% and 32.1% reduction for A. oligospora and A. flagrans, respectively. A concentration of 10 spores per egg resulted in 87.4% and 90.5% reduction, while 100 spores per egg resulted in 95.8% and 93.9% reduction for A. oligospora and A. flagrans, respectively.
Publication Date: 1995-01-01 PubMed ID: 7732642DOI: 10.1016/0304-4017(94)00663-wGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research explores how two types of nematophagous fungi, particularly Arthrobotrys oligospora and Arthrobotrys flagrans, can affect the development of infective cyathostome larvae found in horse feces. The fungi showed significant potential in reducing the larvae concentration, indicating their possible use in parasite control.
Research Objective
- The main purpose of the study was to examine the ability of Arthrobotrys oligospora and Arthrobotrys flagrans to regulate the development of infective larvae in horse feces.
- The horse feces used in this study were naturally infected with cyathostomes, a type of parasitic worm common in horses.
- These horses were from a farm where earlier findings had confirmed that cyathostomes were 100% responsible for the strongyle egg output.
Research Methodology
- The researchers mixed the horse feces with spores of each fungal species at four different concentrations: 0 (control), 1, 10, and 100 spores per egg.
- They then had five replicates for each group incubate for eight days.
- The researchers used a technique called the Baermann method to harvest the infective larvae.
- They counted these larvae and calculated the percentage reduction in infective cyathostome larvae for each level of fungal concentration in comparison to controls.
Research Findings
- The study found out that a fungal concentration of 1 spore per egg resulted in a 40.5% decrease in larvae for Arthrobotrys oligospora and a 32.1% decrease for Arthrobotrys flagrans.
- A concentration of 10 spores per egg resulted in an 87.4% and 90.5% reduction, while 100 spores per egg resulted in 95.8% and 93.9% reduction for A. oligospora and A. flagrans, respectively.
- The results indicate that these fungi may have significant potential for controlling parasitic infections in horses.
Cite This Article
APA
Bird J, Herd RP.
(1995).
In vitro assessment of two species of nematophagous fungi (Arthrobotrys oligospora and Arthrobotrys flagrans) to control the development of infective cyathostome larvae from naturally infected horses.
Vet Parasitol, 56(1-3), 181-187.
https://doi.org/10.1016/0304-4017(94)00663-w Publication
Researcher Affiliations
- Department of Veterinary Preventive Medicine, Ohio State University College of Veterinary Medicine, Columbus 43210-1092, USA.
MeSH Terms
- Animals
- Feces / parasitology
- Horses
- Larva
- Mitosporic Fungi
- Models, Statistical
- Parasite Egg Count
- Spores, Fungal
- Strongyle Infections, Equine / prevention & control
- Strongylus / isolation & purification
Citations
This article has been cited 2 times.- Li S, Wang D, Gong J, Zhang Y. Individual and Combined Application of Nematophagous Fungi as Biological Control Agents against Gastrointestinal Nematodes in Domestic Animals.. Pathogens 2022 Jan 27;11(2).
- de Almeida GL, Santurio JM, Filho JO, Zanette RA, Camillo G, Flores AG, da Silva JH, de la Rue ML. Predatory activity of the fungus Duddingtonia flagrans in equine strongyle infective larvae on natural pasture in the Southern Region of Brazil.. Parasitol Res 2012 Feb;110(2):657-62.
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