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Home / Equine Research Bank / Vet Res Commun / In vitro bioassay to detect reaginic antibodies from the ser...
Veterinary research communications2009; 34(1); 91-99; doi: 10.1007/s11259-009-9334-0

In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction.

Abstract: In horses, Recurrent Airway Obstruction (RAO) is an allergic disease that involves IgE mediated Type I Hypersensitivity responses. The development of this type of allergy involves a series of events that begins with reaginic antibodies, mainly IgE and some IgG subclasses. These reaginic antibodies bind with high affinity, via the Fc portion, to FcepsilonRI receptors on the membrane of mast cells and basophils. Once bound, environmental allergens cross-link the antibodies, which results in mast cell degranulation leading to the production of histamine and other chemical mediators that act together to induce airway inflammation. RAO-affected horses present with coughing, respiratory distress, airway obstruction and poor performance. The aspect of the RAO has been extensively studied, yet the precise sequence of events is still not well understood. Therefore, this study proposes a bioassay for reaginic antibody detection from horse serum of RAO-affected individuals, in order to determine the etiology of disease, which mediate immediate type reactions. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from affected or unaffected horses and one of the RAO antigens (Aspergillus fumigatus). The results presented here demonstrate that 30% of RAO-affected horses react positively in this in vitro bioassay, whereas unaffected horses do not. This bioassay may facilitate further research on RAO and other allergic diseases in horses.
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Publication Date: 2009-12-10 PubMed ID: 20012188DOI: 10.1007/s11259-009-9334-0Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper describes a newly developed bioassay to detect reaginic antibodies in the serum of horses affected by Recurrent Airway Obstruction, an allergic disease. The study’s findings suggest that this new method could provide further insight into this disease and its mechanisms.

Recurrent Airway Obstruction in Horses

  • Recurrent Airway Obstruction (RAO) in horses involves an allergic reaction characterized by IgE mediated Type I Hypersensitivity.
  • The process of developing this allergy involves a specific set of events starting with the formation of reaginic antibodies, primarily IgE and some IgG subclasses.
  • The reaginic antibodies interact with the receptors FcepsilonRI found on mast cells and basophils, which cause cell degranulation, leading to the production of histamine and other chemicals that together provoke airway inflammation.
  • Signs of an RAO-affected horse include coughing, difficulty breathing, airway blockages, and poor performance.

Purpose of the Study

  • While considerable research has been conducted on RAO, the exact sequence of allergic reactions is not yet fully understood.
  • This study aimed to develop a bioassay for detecting reaginic antibodies in the serum of RAO-affected horses to better understand the disease’s progression.

Utilization of Bioassay Technique

  • The proposed bioassay involves measuring in vitro calcium mobilization in RBL-2H3 cells after incubation with horse serum (from affected or unaffected horses) and a RAO antigen, Aspergillus fumigatus.
  • The objective was to determine how these components interact and identify any differences between the serum from affected and unaffected horses.

Findings of the Study

  • The study found that 30% of RAO-affected horses produced a positive reaction in this in vitro bioassay. In contrast, the serum from unaffected horses did not produce any reaction.
  • The findings suggest that this bioassay may be beneficial to further research on RAO and other equine allergic diseases.

Cite This Article

APA
Morán G, Burgos R, Araya O, Folch H. (2009). In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction. Vet Res Commun, 34(1), 91-99. https://doi.org/10.1007/s11259-009-9334-0

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 34
Issue: 1
Pages: 91-99

Researcher Affiliations

Morán, Gabriel
  • Department of Pharmacology, Faculty of Veterinary Science, Universidad Austral de Chile, Valdivia, Chile. gmoran@uach.cl
Burgos, Rafael
    Araya, Oscar
      Folch, Hugo

        MeSH Terms

        • Airway Obstruction / immunology
        • Airway Obstruction / veterinary
        • Animals
        • Antigens, Fungal
        • Aspergillus fumigatus / immunology
        • Biological Assay / methods
        • Calcium / metabolism
        • Cell Line
        • Horse Diseases / immunology
        • Horses
        • Reagins / blood

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        Citations

        This article has been cited 5 times.
        1. Sabban S, Ye H, Helm B. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI.. J Vis Exp 2014 Nov 1;(93):e52222.
          doi: 10.3791/52222pubmed: 25406512google scholar: lookup
        2. Sarmiento J, Perez B, Morales N, Henriquez C, Vidal L, Folch H, Galecio JS, Morán G. Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid.. Vet Res Commun 2013 Dec;37(4):333-8.
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        3. Moran G, Folch H, Henriquez C, Ortloff A, Barria M. Reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation.. Vet Res Commun 2012 Dec;36(4):251-8.
          doi: 10.1007/s11259-012-9534-xpubmed: 23011757google scholar: lookup
        4. Moran G, Buechner-Maxwell VA, Folch H, Henriquez C, Galecio JS, Perez B, Carrasco C, Barria M. Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction.. Vet Res Commun 2011 Oct;35(7):447-56.
          doi: 10.1007/s11259-011-9482-xpubmed: 21594641google scholar: lookup
        5. Morán G, Folch H, Araya O, Burgos R, Barria M. Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay.. Vet Res Commun 2010 Dec;34(8):719-26.
          doi: 10.1007/s11259-010-9440-zpubmed: 20814741google scholar: lookup
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