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Neutrophilic airway inflammation in horses with heaves is characterized by a Th2-type cytokine profile.
Abstract: Heaves in horses shares many similarities with human asthma, including lower airway inflammation, reversible airway obstruction, and bronchial hyperresponsiveness. Extrinsic asthma is an allergic response to environmental allergens and a similar immunologic mechanism may be implicated in heaves. It is now recognized that a Th2 subset of CD4+ lymphocytes is associated with allergic diseases such as atopic asthma. The purpose of this study was to determine whether airway inflammation in heaves is associated with a pattern of expression of cytokine suggestive of a Th2 type response. The expression of mRNA, encoding interleukin (IL)-4, IL-5, and interferon gamma (IFN-gamma) was measured in bronchoalveolar cells from seven horses with heaves and five control horses, using in situ hybridization and radiolabeled equine-specific cRNA probes coding for these cytokines. Bronchoalveolar cells of horses with heaves had an increased expression of IL-4 (p = 0.01) and IL-5 (p = 0.02) mRNA and a decreased expression of INF-gamma (p = 0.01) compared with control horses. Here we show that inflammatory cells in lungs from horses with heaves display a Th2-type cytokine profile that is consistent with the hypothesis that heaves is an allergic condition with similarity to human asthma.
Publication Date: 2001-11-13 PubMed ID: 11704587DOI: 10.1164/ajrccm.164.8.2012091Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The article reviews a study aimed at understanding the type of immune response (Th2-type) associated with a respiratory disease in horses, commonly called ‘heaves’, which holds similarities to human asthma. It focuses on analyzing the expression of specific cytokines in bronchoalveolar cells from both affected and unaffected horses.
Introduction
- The study explores heaves in horses, which is similar to human asthma including lower airway inflammation, reversible airway obstruction, and bronchial hyperresponsiveness.
- The disease can be associated with an allergic response to environmental allergens, implying an immunologic mechanism at play.
- The research aims to establish if inflammation in heaves is linked with a Th2 type response, a subset of CD4+ lymphocytes associated with allergic diseases.
Methods
- The study measures the expression of mRNA – encoding interleukin (IL)-4, IL-5, and interferon gamma (IFN-gamma) – in bronchoalveolar cells from seven horses with heaves and five control horses.
- This is done by using in situ hybridization and radiolabeled equine-specific cRNA probes that code for these cytokines.
Findings
- The bronchoalveolar cells of horses diagnosed with heaves showed increased expression of IL-4 and IL-5 mRNA, with decreased expression of INF-gamma, in comparison to the control horses.
- This suggests that the immune cells in the lungs of horses with heaves present a Th2 type cytokine profile.
Conclusion
- The results of the study align with the hypothesis that heaves is an allergic condition exhibiting a Th2-type cytokine profile, akin to human asthma.
- This understanding can have implications on the management and potential treatment of the disease, contributing to equine health.
Cite This Article
APA
Lavoie JP, Maghni K, Desnoyers M, Taha R, Martin JG, Hamid QA.
(2001).
Neutrophilic airway inflammation in horses with heaves is characterized by a Th2-type cytokine profile.
Am J Respir Crit Care Med, 164(8 Pt 1), 1410-1413.
https://doi.org/10.1164/ajrccm.164.8.2012091 Publication
Researcher Affiliations
- Facultu00e9 de Mu00e9decine Vu00e9tu00e9rinaire, Universitu00e9 de Montru00e9al, St-Hyacinthe, Quu00e9bec, Canada. Jean-Pierre.Lavoie@umontreal.ca
MeSH Terms
- Animals
- Asthma / immunology
- Asthma / veterinary
- Bronchoalveolar Lavage Fluid / cytology
- Cytokines / biosynthesis
- Cytokines / genetics
- Horse Diseases / immunology
- Horses
- Inflammation / immunology
- Inflammation / veterinary
- Neutrophils / immunology
- RNA, Messenger / biosynthesis
- Th2 Cells / immunology
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