In vitro development of day-2 equine embryos co-cultured with oviductal explants or trophoblastic vesicles.
Abstract: This study compared the in vitro development of Day-2 equine embryos co-cultured with either trophoblastic vesicles or oviductal explants. Embryos were collected surgically from the oviducts of pony mares 2 d after ovulation and assessed for stage of development. Culture medium was Ham's F12 and Dulbecco's Modified Eagle's Medium (50:50 v/v) in a humidified atmosphere of 5% CO(2) in air at 38.5 degrees C with either trophoblastic vesicles or oviductal explants. The quality score of embryos was assessed daily. After 4 d in culture, embryos were stained (Hoechst 33342) and evaluated with epifluorescence to determine the number of nuclei present. Six of seven embryos co-cultured with oviductal examples developed to the morula/blastocyst stage, while four of seven embryos co-cultured with trophoblastic vesicles developed to the morula stage. More (P = 0.1) embryos co-cultured with oviductal explants reached the blastocyst stage than embryos co-cultured with trophoblastic vesicles (3/7 vs 0/7, respectively). The number of cells was higher (P = 0.1) for embryos co-cultured with oviductal explants than for embryos co-cultured with trophoblastic vesicles (162.6 +/- 32 vs 87.3 +/- 28, respectively). The number of cells for embryos co-cultured with either oviductal explants or trophoblastic vesicles appeared to be lower than for embryos matured in vivo that were recovered from the uterus at Day 6 (378, 399, >1000). The co-culture of early equine embryos in a completely defined medium with either trophoblastic vesicles or oviductal explants can support development to at least the morula stage. The co-culture of embryos with oviductal explants resulted in superior development of four-to eight-cell embryos, as indicated by the proportion that reached the blastocyst stage and by the number of cells.
Publication Date: 1991-03-01 PubMed ID: 16726935DOI: 10.1016/0093-691x(91)90462-mGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research explores the impact of co-culturing day-2 equine embryos with either trophoblastic vesicles or oviductal explants on embryo development, with the results indicating superior development results from co-culturing with oviductal explants.
Introduction of Research
- This research focused on assessing the effect of two distinct co-culture environments on in vitro development of Day-2 equine embryos.
- Embryos were surgically retrieved from pony mares post ovulation and then developed under specific lab conditions.
- The embryos were cultured using a blend of Ham’s F12 and Dulbecco’s Modified Eagle’s Medium in a humidified environment of 5% carbon dioxide, co-cultured with either trophoblastic vesicles or oviductal explants.
Methodology and Assessments
- The embryos were then evaluated for their stage of development through daily quality score checks.
- Epifluorescence, using the Hoechst 33342 stain, was applied after four days of culture to determine the number of nuclei within the embryos.
Results of the Study
- The research showcased how out of seven embryos co-cultured with oviductal examples, six developed up to the morula/blastocyst stage, while out of seven embryos co-cultured with trophoblastic vesicles, four developed to the morula stage.
- Of the embryos co-cultured alongside oviductal explants, more reached the blastocyst stage than those co-cultured with trophoblastic vesicles (3 out of 7 vs 0 out of 7 respectively).
- Embryos co-cultured with oviductal explants also exhibited a higher cell count than those co-cultured with trophoblastic vesicles.
Conclusion
- The study concluded that co-culture of early equine embryos with either oviductal explants or trophoblastic vesicles in a defined medium can support development to at least the morula stage.
- It was also found that embryos co-cultured with oviductal explants exhibited superior development in the four-to eight-cell stage, as per the proportion reaching the blastocyst stage and their cell number, in comparison to those co-cultured with trophoblastic vesicles.
Cite This Article
APA
Ball BA, Altschul M, Ellington JE.
(1991).
In vitro development of day-2 equine embryos co-cultured with oviductal explants or trophoblastic vesicles.
Theriogenology, 35(3), 669-682.
https://doi.org/10.1016/0093-691x(91)90462-m Publication
Researcher Affiliations
- Department of Clinical Sciences, Cornell University, Ithaca, NY 14853, USA.
Citations
This article has been cited 1 times.- Ma Y, Gu M, Chen L, Shen H, Pan Y, Pang Y, Miao S, Tong R, Huang H, Zhu Y, Sun L. Recent advances in critical nodes of embryo engineering technology. Theranostics 2021;11(15):7391-7424.
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