In vitro effects of lidocaine on contractility of circular and longitudinal equine intestinal smooth muscle.
Abstract: The purpose of the study was to compare the contractility-enhancing effects of lidocaine in equine jejunal circular (CSM) and longitudinal smooth muscle (LSM) in vitro. In previous studies, more pronounced effects of lidocaine were observed in ischaemia-reperfusion (IR) injured smooth muscle. Therefore in this study, effects were examined in both non-injured control tissues and tissues challenged by a defined, artificial IR injury. Isometric contractile performance of CSM and LSM, assessed by frequency (F), amplitude (A) and mean active force (MAF) of contractions, was defined as contractility. LSM featured lower basic contractility compared to CSM. Lidocaine provoked contractility-enhancing effects in both smooth muscle layers, but except for F at high lidocaine concentrations, contractility of LSM remained lower throughout the trial. Additionally, higher lidocaine concentrations were required to cause significant effects in LSM. No differences were observed in contractility of control and IR injured smooth muscle, but higher lidocaine concentrations were needed to provoke effects in IR injured smooth muscle. In contrast to CSM, contractility of LSM did not decrease at comparably high lidocaine concentrations. Differences in basic contractility of CSM and LSM might be explained by physiologically lower activity of LSM per se or by a thinner LSM layer with fewer smooth muscle cells taking part in contractions. The smaller thickness of the LSM layer may also contribute to persisting discrepancies in contractility following lidocaine application. Additionally, variations in lidocaine concentrations necessary for inducing significant effects could result from differences in the molecular structure of CSM and LSM cells.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication Date: 2013-08-22 PubMed ID: 23973159DOI: 10.1016/j.tvjl.2013.07.027Google Scholar: Lookup
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- Journal Article
Summary
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The paper investigates the varying impacts of lidocaine—a type of medication often used to numb tissue in a specific area—on the contractility of circular and longitudinal smooth muscle in horse intestines both in normal state and under artificial ischemia-reperfusion injury conditions. It found that the drug enhances contractility in both types of muscle but with different effective concentration levels and responses under injury conditions.
Objective of the Study
- The research aimed to compare the contractility-enhancing effects of lidocaine on circular smooth muscle (CSM) and longitudinal smooth muscle (LSM) of horse intestines.
- It investigated these effects in both normally functioning tissues and those affected by ischemia-reperfusion (IR) injury—a condition caused by temporary restriction of blood supply.
Methodology and Key Observations
- The contractility of the muscles, determined by analyzing frequency, amplitude, and mean active force of contractions, was tested.
- The LSM had inherently less contractility compared to the CSM.
- Lidocaine enhanced contractility in both muscles, but a higher concentration of the drug was needed to significantly affect the LSM.
- Unlike for the CSM, contractility of the LSM did not decrease even at high concentrations of lidocaine.
Impact of Ischaemia-Reperfusion Injury
- The effects of lidocaine on both control tissues and those subjected to an artificial IR injury were compared.
- Contractility between the control and IR-injured smooth muscle tissue showed no notable differences, but higher concentrations of lidocaine were required to induce effects in the injured tissue.
Potential Explanations for the Observations
- The differing contractility between CSM and LSM might be due to inherent physiological variations or differences in thickness, with LSM possibly having fewer muscle cells involved in contractions due to thinner layers.
- The different concentrations of lidocaine required to induce contractility changes could be due to variations in the molecular structure of the CSM and LSM cells.
Cite This Article
APA
Tappenbeck K, Hoppe S, Reichert C, Feige K, Huber K.
(2013).
In vitro effects of lidocaine on contractility of circular and longitudinal equine intestinal smooth muscle.
Vet J, 198(1), 170-175.
https://doi.org/10.1016/j.tvjl.2013.07.027 Publication
Researcher Affiliations
- Department of Physiology, University of Veterinary Medicine, Bischofsholer Damm 15, D-30173 Hannover, Germany.
MeSH Terms
- Anesthetics, Local / pharmacokinetics
- Anesthetics, Local / therapeutic use
- Animals
- Female
- Horse Diseases / drug therapy
- Horse Diseases / pathology
- Horses
- In Vitro Techniques
- Isometric Contraction
- Jejunum / drug effects
- Jejunum / pathology
- Lidocaine / pharmacokinetics
- Lidocaine / therapeutic use
- Male
- Muscle, Smooth / blood supply
- Muscle, Smooth / drug effects
- Muscle, Smooth / pathology
- Reperfusion Injury / drug therapy
- Reperfusion Injury / pathology
- Reperfusion Injury / veterinary
Citations
This article has been cited 7 times.- Mahalingam-Dhingra A, Mazan MR, Bedenice D, Ceresia M, Minuto J, Deveney EF. A CONSORT-guided, randomized, double-blind, controlled pilot clinical trial of inhaled lidocaine for the treatment of equine asthma. Can J Vet Res 2022 Apr;86(2):116-124.
- Röhm K, Diener M, Huber K, Seifert J. Characterization of Cecal Smooth Muscle Contraction in Laying Hens. Vet Sci 2021 May 26;8(6).
- Lv X, Li X, Guo K, Li T, Yang Y, Lu W, Wang S, Liu S. Effects of Systemic Lidocaine on Postoperative Recovery Quality and Immune Function in Patients Undergoing Laparoscopic Radical Gastrectomy. Drug Des Devel Ther 2021;15:1861-1872.
- Beaussier M, Delbos A, Maurice-Szamburski A, Ecoffey C, Mercadal L. Perioperative Use of Intravenous Lidocaine. Drugs 2018 Aug;78(12):1229-1246.
- Salem SE, Proudman CJ, Archer DC. Has intravenous lidocaine improved the outcome in horses following surgical management of small intestinal lesions in a UK hospital population?. BMC Vet Res 2016 Jul 27;12(1):157.
- Zhang P, Zhao Y, Mu H, Zhang J, Zhang F, Wang S. Compare the Effects of Transversalis Fascia Plane Block versus Intravenous Lidocaine Infusion on the Quality of Early Postoperative Recovery in Patients Undergoing Gynecologic Laparoscopic Surgery. J Pain Res 2025;18:7019-7027.
- Wu Y, Chen Z, Yao C, Sun H, Li H, Du X, Cheng J, Wan X. Effect of systemic lidocaine on postoperative quality of recovery, the gastrointestinal function, inflammatory cytokines of lumbar spinal stenosis surgery: a randomized trial. Sci Rep 2023 Oct 17;13(1):17661.
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