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Home / Equine Research Bank / Front Vet Sci / In vitro Effects of Methylprednisolone Acetate on Equine Dee...
Frontiers in veterinary science2020; 7; 486; doi: 10.3389/fvets.2020.00486

In vitro Effects of Methylprednisolone Acetate on Equine Deep Digital Flexor Tendon-Derived Cells.

Abstract: Primary deep digital flexor tendon (DDFT) pathologies and those accompanying degenerative changes of navicular bone fibrocartilage are major causes of lameness associated with navicular disease. Intrasynovial corticosteroids are mainstay in the treatment due to the anti-inflammatory effects, but their effect on DDFT cell biosynthesis are unknown. The objective of this study was to investigate the effects of methylprednisolone acetate (MPA) on cells isolated from the dorsal fibrocartilaginous region of forelimb DDFTs (DDFT-derived cells) of 5 horses (aged 11-17 years). Non-adherent aggregate cultures were established from third passage cells over a 72 to 96-h duration prior to treating with medium containing 0 (control), 0.05 and 0.5 mg/mL MPA for 24 h. Tendon and cartilage extracellular matrix (ECM) related gene expression, cell aggregate and culture medium GAG contents, culture medium collagen and MMP-3 and-13 concentrations were measured. After 24 h of treatment, only the higher MPA concentration (0.5 mg/mL) significantly down-regulated tendon ECM related genes; whereas, both MPA doses significantly down-regulated cartilage ECM related genes. MPA treatment did not affect the total GAG content of DDFT-derived cells or total GAG, soluble collagen and MMP-3 and-13 contents in culture medium compared to untreated controls. Future studies to determine the response of DDFT-derived cells with longer exposure times to corticosteroids and in the presence of inflammatory cytokines are necessary. These results are a first step in assessing the effects of intrasynovial medications on equine DDFT, for which currently no information exists.
Copyright © 2020 Sullivan, Altmann, Brokken and Durgam.
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Publication Date: 2020-08-05 PubMed ID: 32851046PubMed Central: PMC7419577DOI: 10.3389/fvets.2020.00486Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the impact of methylprednisolone acetate (MPA) on the deep digital flexor tendon (DDFT) cells in horses, which plays a major role in cases of equine lameness associated with navicular diseases.

Background

  • DDFT pathologies, along with degenerative changes of navicular bone fibrocartilage cause lameness associated with navicular disease in horses. Intrasynovial corticosteroids are commonly used as a treatment due to their anti-inflammatory properties.
  • The effect of these corticosteroids on DDFT cell biosynthesis is not yet fully understood. This study aims to examine the impact of MPA on the cells derived from the DDFT of horses.

Methodology

  • The researchers sourced cells from the dorsal fibrocartilaginous region of forelimb DDFTs from 5 horses aged between 11-17 years. The extracted cells underwent aggregate culture over a period of 72 to 96 hours prior to treatment.
  • After the cultures were established, they were treated with MPA at concentrations of 0 (control), 0.05 and 0.5 mg/mL for a duration of 24 hours. They then measured levels of tendon and cartilage extracellular matrix (ECM) related gene expression, cell aggregate and culture GAG contents, and the concentrations of culture medium collagen and MMP-3 and -13.

Findings

  • At a higher MPA concentration, tendon ECM-related genes were significantly down-regulated. However, both dosages of MPA significantly down-regulated cartilage ECM related genes.
  • MPA did not affect the total GAG content of DDFT-derived cells or the total GAG, soluble collagen, and MMP-3 and -13 contents in culture medium, compared to untreated controls.

Conclusions and Future Directions

  • The researchers suggest future studies to examine the response of DDFT-derived cells to longer exposure times to corticosteroids and in the presence of inflammatory cytokines to further understand the effects.
  • This research forms the initial stages of understanding the impact of intrasynovial medications on equine DDFT, which is currently lacking in the scientific field.

Cite This Article

APA
Sullivan SN, Altmann NN, Brokken MT, Durgam SS. (2020). In vitro Effects of Methylprednisolone Acetate on Equine Deep Digital Flexor Tendon-Derived Cells. Front Vet Sci, 7, 486. https://doi.org/10.3389/fvets.2020.00486

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 7
Pages: 486
PII: 486

Researcher Affiliations

Sullivan, Stasia N
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Altmann, Nadine N
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Brokken, Matthew T
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Durgam, Sushmitha S
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.

References

This article includes 43 references
  1. Murray RC, Schramme MC, Dyson SJ, Branch MV, Blunden TS. Magnetic resonance imaging characteristics of the foot in horses with palmar foot pain and control horses.. Vet Radiol Ultrasound 2006 Jan-Feb;47(1):1-16.
    doi: 10.1111/j.1740-8261.2005.00100.xpubmed: 16429980google scholar: lookup
  2. Blunden A, Murray R, Dyson S. Lesions of the deep digital flexor tendon in the digit: a correlative MRI and post mortem study in control and lame horses.. Equine Vet J 2009 Jan;41(1):25-33.
    doi: 10.2746/042516408X343028pubmed: 19301578google scholar: lookup
  3. Blunden A, Dyson S, Murray R, Schramme M. Histopathology in horses with chronic palmar foot pain and age-matched controls. Part 2: The deep digital flexor tendon.. Equine Vet J 2006 Jan;38(1):23-7.
    doi: 10.2746/042516406775374342pubmed: 16411582google scholar: lookup
  4. Blunden A, Dyson S, Murray R, Schramme M. Histopathology in horses with chronic palmar foot pain and age-matched controls. Part 1: Navicular bone and related structures.. Equine Vet J 2006 Jan;38(1):15-22.
    doi: 10.2746/042516406775374298pubmed: 16411581google scholar: lookup
  5. Marsh CA, Schneider RK, Sampson SN, Roberts GD. Response to injection of the navicular bursa with corticosteroid and hyaluronan following high-field magnetic resonance imaging in horses with signs of navicular syndrome: 101 cases (2000-2008).. J Am Vet Med Assoc 2012 Nov 15;241(10):1353-64.
    doi: 10.2460/javma.241.10.1353pubmed: 23113529google scholar: lookup
  6. de Grauw JC, Visser-Meijer MC, Lashley F, Meeus P, van Weeren PR. Intra-articular treatment with triamcinolone compared with triamcinolone with hyaluronate: A randomised open-label multicentre clinical trial in 80 lame horses.. Equine Vet J 2016 Mar;48(2):152-8.
    doi: 10.1111/evj.12383pubmed: 25377505google scholar: lookup
  7. Gutierrez-Nibeyro SD, White Ii NA, Werpy NM. Outcome of medical treatment for horses with foot pain: 56 cases.. Equine Vet J 2010 Nov;42(8):680-5.
    doi: 10.1111/j.2042-3306.2010.00081.xpubmed: 21039796google scholar: lookup
  8. Manfredi JM, Boyce M, Malone ED, Anderson C, Anderson LB, Trumble TN. Steroid diffusion into the navicular bursa occurs in horses affected by palmar foot pain.. Vet Rec 2012 Dec 22-29;171(25):642.
    doi: 10.1136/vr.101075pubmed: 23136308google scholar: lookup
  9. Boyce M, Malone ED, Anderson LB, Park S, Godden SM, Jenner F, Trumble TN. Evaluation of diffusion of triamcinolone acetonide from the distal interphalangeal joint into the navicular bursa in horses.. Am J Vet Res 2010 Feb;71(2):169-75.
    doi: 10.2460/ajvr.71.2.169pubmed: 20113224google scholar: lookup
  10. Pauwels FE, Schumacher J, Castro FA, Holder TE, Carroll RC, Sega GA, Rogers CW. Evaluation of the diffusion of corticosteroids between the distal interphalangeal joint and navicular bursa in horses.. Am J Vet Res 2008 May;69(5):611-6.
    doi: 10.2460/ajvr.69.5.611pubmed: 18447791google scholar: lookup
  11. Kawcak CE, Norrdin RW, Frisbie DD, Trotter GW, Mcilwraith CW. Effects of osteochondral fragmentation and intra-articular triamcinolone acetonide treatment on subchondral bone in the equine carpus.. Equine Vet J 1998 Jan;30(1):66-71.
    doi: 10.1111/j.2042-3306.1998.tb04090.xpubmed: 9458401google scholar: lookup
  12. Frisbie DD, Kawcak CE, Baxter GM, Trotter GW, Powers BE, Lassen ED, McIlwraith CW. Effects of 6alpha-methylprednisolone acetate on an equine osteochondral fragment exercise model.. Am J Vet Res 1998 Dec;59(12):1619-28.
    pubmed: 9858417
  13. Frisbie DD, Kawcak CE, Trotter GW, Powers BE, Walton RM, McIlwraith CW. Effects of triamcinolone acetonide on an in vivo equine osteochondral fragment exercise model.. Equine Vet J 1997 Sep;29(5):349-59.
    doi: 10.1111/j.2042-3306.1997.tb03138.xpubmed: 9306060google scholar: lookup
  14. Richardson DW, Dodge GR. Dose-dependent effects of corticosteroids on the expression of matrix-related genes in normal and cytokine-treated articular chondrocytes.. Inflamm Res 2003 Jan;52(1):39-49.
    doi: 10.1007/s000110300012pubmed: 12608648google scholar: lookup
  15. Busschers E, Holt JP, Richardson DW. Effects of glucocorticoids and interleukin-1 beta on expression and activity of aggrecanases in equine chondrocytes.. Am J Vet Res 2010 Feb;71(2):176-85.
    doi: 10.2460/ajvr.71.2.176pubmed: 20113225google scholar: lookup
  16. Dechant JE, Baxter GM, Frisbie DD, Trotter GW, McIlwraith CW. Effects of dosage titration of methylprednisolone acetate and triamcinolone acetonide on interleukin-1-conditioned equine articular cartilage explants in vitro.. Equine Vet J 2003 Jul;35(5):444-50.
    doi: 10.2746/042516403775600479pubmed: 12875321google scholar: lookup
  17. Wong MW, Tang YY, Lee SK, Fu BS. Glucocorticoids suppress proteoglycan production by human tenocytes.. Acta Orthop 2005 Dec;76(6):927-31.
    doi: 10.1080/17453670610046118pubmed: 16470453google scholar: lookup
  18. Wong MW, Tang YN, Fu SC, Lee KM, Chan KM. Triamcinolone suppresses human tenocyte cellular activity and collagen synthesis.. Clin Orthop Relat Res 2004 Apr;(421):277-81.
    doi: 10.1097/01.blo.0000118184.83983.65pubmed: 15123960google scholar: lookup
  19. Wong MW, Tang YY, Lee SK, Fu BS, Chan BP, Chan CK. Effect of dexamethasone on cultured human tenocytes and its reversibility by platelet-derived growth factor.. J Bone Joint Surg Am 2003 Oct;85(10):1914-20.
    doi: 10.2106/00004623-200310000-00008pubmed: 14563798google scholar: lookup
  20. Tempfer H, Gehwolf R, Lehner C, Wagner A, Mtsariashvili M, Bauer HC, Resch H, Tauber M. Effects of crystalline glucocorticoid triamcinolone acetonide on cultered human supraspinatus tendon cells.. Acta Orthop 2009 Jun;80(3):357-62.
    doi: 10.3109/17453670902988360pmc: PMC2823208pubmed: 19421912google scholar: lookup
  21. Wei AS, Callaci JJ, Juknelis D, Marra G, Tonino P, Freedman KB, Wezeman FH. The effect of corticosteroid on collagen expression in injured rotator cuff tendon.. J Bone Joint Surg Am 2006 Jun;88(6):1331-8.
    doi: 10.2106/JBJS.E.00806pmc: PMC3071041pubmed: 16757768google scholar: lookup
  22. Sherlock C, Mair T, Blunden T. Deep erosions of the palmar aspect of the navicular bone diagnosed by standing magnetic resonance imaging.. Equine Vet J 2008 Nov;40(7):684-92.
    doi: 10.2746/042516408X330365pubmed: 19165939google scholar: lookup
  23. Beck S, Blunden T, Dyson S, Murray R. Are matrix and vascular changes involved in the pathogenesis of deep digital flexor tendon injury in the horse?. Vet J 2011 Sep;189(3):289-95.
    doi: 10.1016/j.tvjl.2010.07.015pubmed: 21821448google scholar: lookup
  24. Vogel KG. Tendon structure and response to changing mechanical load.. J Musculoskelet Neuronal Interact 2003 Dec;3(4):323-5; discussion 333-4.
    pubmed: 15758312
  25. Durgam SS, Stewart AA, Pondenis HC, Gutierrez-Nibeyro SM, Evans RB, Stewart MC. Comparison of equine tendon- and bone marrow-derived cells cultured on tendon matrix with or without insulin-like growth factor-I supplementation.. Am J Vet Res 2012 Jan;73(1):153-61.
    doi: 10.2460/ajvr.73.1.153pubmed: 22204302google scholar: lookup
  26. Quam V, Altmann N, coughlin H, Brokken M, Durgam S. Tendon progenitor cells isolated from equine deep digital flexor tendon at the navicular region are restricted to a chondrogenic phenotype. Veterinary Surgery Las Vegas, NV (2019).
  27. Stewart MC, Saunders KM, Burton-Wurster N, Macleod JN. Phenotypic stability of articular chondrocytes in vitro: the effects of culture models, bone morphogenetic protein 2, and serum supplementation.. J Bone Miner Res 2000 Jan;15(1):166-74.
    doi: 10.1359/jbmr.2000.15.1.166pubmed: 10646126google scholar: lookup
  28. Yates AC, Stewart AA, Byron CR, Pondenis HC, Kaufmann KM, Constable PD. Effects of sodium hyaluronate and methylprednisolone acetate on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1.. Am J Vet Res 2006 Dec;67(12):1980-6.
    doi: 10.2460/ajvr.67.12.1980pubmed: 17144797google scholar: lookup
  29. Byron CR, Benson BM, Stewart AA, Pondenis HC. Effects of methylprednisolone acetate and glucosamine on proteoglycan production by equine chondrocytes in vitro.. Am J Vet Res 2008 Sep;69(9):1123-8.
    doi: 10.2460/ajvr.69.9.1123pubmed: 18764681google scholar: lookup
  30. Edmonds RE, Garvican ER, Smith RK, Dudhia J. Influence of commonly used pharmaceutical agents on equine bone marrow-derived mesenchymal stem cell viability.. Equine Vet J 2017 May;49(3):352-357.
    doi: 10.1111/evj.12590pubmed: 27160051google scholar: lookup
  31. Durgam S, Schuster B, Cymerman A, Stewart A, Stewart M. Differential Adhesion Selection for Enrichment of Tendon-Derived Progenitor Cells During In Vitro Culture.. Tissue Eng Part C Methods 2016 Aug;22(8):801-8.
    doi: 10.1089/ten.tec.2016.0152pubmed: 27406327google scholar: lookup
  32. Durgam SS, Stewart AA, Pondenis HC, Yates AC, Evans RB, Stewart MC. Responses of equine tendon- and bone marrow-derived cells to monolayer expansion with fibroblast growth factor-2 and sequential culture with pulverized tendon and insulin-like growth factor-I.. Am J Vet Res 2012 Jan;73(1):162-70.
    doi: 10.2460/ajvr.73.1.162pubmed: 22204303google scholar: lookup
  33. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.. Methods 2001 Dec;25(4):402-8.
    doi: 10.1006/meth.2001.1262pubmed: 11846609google scholar: lookup
  34. Farndale RW, Sayers CA, Barrett AJ. A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.. Connect Tissue Res 1982;9(4):247-8.
    doi: 10.3109/03008208209160269pubmed: 6215207google scholar: lookup
  35. Durgam SS, Stewart AA, Sivaguru M, Wagoner Johnson AJ, Stewart MC. Tendon-derived progenitor cells improve healing of collagenase-induced flexor tendinitis.. J Orthop Res 2016 Dec;34(12):2162-2171.
    doi: 10.1002/jor.23251pubmed: 27035120google scholar: lookup
  36. Caron JP, Gandy JC, Schmidt M, Hauptman JG, Sordillo LM. Influence of corticosteroids on interleukin-1β-stimulated equine chondrocyte gene expression.. Vet Surg 2013 Apr;42(3):231-7.
    doi: 10.1111/j.1532-950X.2012.01025.xpubmed: 23278565google scholar: lookup
  37. Lutter JD, Schneider RK, Sampson SN, Cary JA, Roberts GD, Vahl CI. Medical treatment of horses with deep digital flexor tendon injuries diagnosed with high-field-strength magnetic resonance imaging: 118 cases (2000-2010).. J Am Vet Med Assoc 2015 Dec 1;247(11):1309-18.
    doi: 10.2460/javma.247.11.1309pubmed: 26594815google scholar: lookup
  38. Maman E, Yehuda C, Pritsch T, Morag G, Brosh T, Sharfman Z, Dolkart O. Detrimental Effect of Repeated and Single Subacromial Corticosteroid Injections on the Intact and Injured Rotator Cuff: A Biomechanical and Imaging Study in Rats.. Am J Sports Med 2016 Jan;44(1):177-82.
    doi: 10.1177/0363546515591266pubmed: 26216105google scholar: lookup
  39. Dinhane KGI, Godoy-Santos AL, Fabro AT, Moretto MR, Deprá I, Yoshida WB. Short-term Changes After Corticosteroid Injections Into the Normal Tendons of Rabbits: A Controlled Randomized Study.. Am J Sports Med 2019 Mar;47(3):721-728.
    doi: 10.1177/0363546518818819pubmed: 30640506google scholar: lookup
  40. Riley G. The pathogenesis of tendinopathy. A molecular perspective.. Rheumatology (Oxford) 2004 Feb;43(2):131-42.
    doi: 10.1093/rheumatology/keg448pubmed: 12867575google scholar: lookup
  41. Muto T, Kokubu T, Mifune Y, Inui A, Harada Y, Yoshifumi, Takase F, Kuroda R, Kurosaka M. Temporary inductions of matrix metalloprotease-3 (MMP-3) expression and cell apoptosis are associated with tendon degeneration or rupture after corticosteroid injection.. J Orthop Res 2014 Oct;32(10):1297-304.
    doi: 10.1002/jor.22681pubmed: 24985902google scholar: lookup
  42. Sendzik J, Shakibaei M, Schäfer-Korting M, Lode H, Stahlmann R. Synergistic effects of dexamethasone and quinolones on human-derived tendon cells.. Int J Antimicrob Agents 2010 Apr;35(4):366-74.
    doi: 10.1016/j.ijantimicag.2009.10.009pubmed: 20034766google scholar: lookup
  43. Lee HJ, Kim YS, Ok JH, Lee YK, Ha MY. Effect of a single subacromial prednisolone injection in acute rotator cuff tears in a rat model.. Knee Surg Sports Traumatol Arthrosc 2015 Feb;23(2):555-61.
    doi: 10.1007/s00167-013-2395-1pubmed: 23370982google scholar: lookup

Citations

This article has been cited 1 times.
  1. Quam VG, Altmann NN, Brokken MT, Durgam SS. Zonal characterization and differential trilineage potentials of equine intrasynovial deep digital flexor tendon-derived cells.. BMC Vet Res 2021 Apr 1;17(1):138.
    doi: 10.1186/s12917-021-02793-1pubmed: 33794882google scholar: lookup
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