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BMC veterinary research2016; 12(1); 135; doi: 10.1186/s12917-016-0759-8

In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide.

Abstract: Platelet-rich plasma (PRP) preparations are a common treatment in equine osteoarthritis (OA). However, there are controversies regarding the ideal concentration of platelets and leukocytes in these biological substances necessary to induce an adequate anti-inflammatory and anabolic response in articular cartilage. The aims were to study the influence of leukocyte- and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG) supernatants on the histological changes of cartilage, the degree of chondrocyte apoptosis, the production of hyaluronan (HA) and the gene expression of nuclear factor kappa beta (NFkβ), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1) and cartilage oligomeric matrix protein (COMP) in normal cartilage explants (CEs) challenged with lipopolysaccharide (LPS). Results: Overall, 25 % L-PRG supernatant (followed in order of importance by, 50 % P-PRG, 25 % P-PRG and 50 % L-PRG) represented the substance with the most important anti-inflammatory and anabolic effect. 25 % P-PRG supernatant presented important anabolic effects, but it induced a more severe chondrocyte apoptosis than the other evaluated substances. Conclusions: 25 % L-PRG supernatant presented the best therapeutic profile. Our results demonstrate that the biological variability of PRP preparations makes their application rather challenging. Additional in vivo research is necessary to know the effect of PRP preparations at different concentrations.
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Publication Date: 2016-07-01 PubMed ID: 27369779PubMed Central: PMC4929746DOI: 10.1186/s12917-016-0759-8Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the different impacts of platelet-rich gel (PRG) preparations on the treatment of equine osteoarthritis and identified that the most effective treatment combination was 25% L-PRG supernatant in terms of promoting anti-inflammatory and anabolic responses.

Understanding the Study

  • Biomedical scientists aimed to understand how different concentrations and types of platelet-rich plasma (PRP) preparations could impact the treatment of osteoarthritis (OA) in horses. PRP preparations are biological substances often used for such treatments.
  • They focused on two specific kinds of PRPs – leukocyte- and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG).
  • The study’s main objectives were to investigate the impacts of these substances on the following parameters:
    • Changes in cartilage tissue.
    • Chondrocyte apoptosis (or cell death) levels.
    • Production of hyaluronan (HA), a substance that helps maintain cartilage flexibility and strength.
    • The genetic expression of several molecules that play a crucial role in cartilage health and OA, including nuclear factor kappa beta (NFkβ), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP).

Results of the Study

  • The researchers found that the most effective PRG preparation for inciting anti-inflammatory and anabolic (or tissue building) effects was 25% L-PRG supernatant, followed by 50% P-PRG, 25% P-PRG, and 50% L-PRG.
  • Interestingly, they noted that while the 25% P-PRG supernatant showed significant anabolic benefits, it also caused more severe chondrocyte apoptosis compared to the other PRG preparations studied.

Conclusion and Implications

  • The researchers determined that the use of 25% L-PRG supernatant presented the most effective balance of therapeutic benefits.
  • However, they acknowledged that the inherent biological variability in PRP preparations makes it challenging to apply them in a standardized way, indicating the need for further research, particularly in vivo (or within the living organism) experiments.
  • The research underscores the importance of identifying optimal PRP preparation concentrations for improving osteoarthritis treatment in horses and potentially other animals as well.

Cite This Article

APA
Carmona JU, Ríos DL, López C, Álvarez ME, Pérez JE, Bohórquez ME. (2016). In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide. BMC Vet Res, 12(1), 135. https://doi.org/10.1186/s12917-016-0759-8

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 12
Issue: 1
Pages: 135

Researcher Affiliations

Carmona, Jorge U
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. carmona@ucaldas.edu.co.
Ríos, Diana L
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
López, Catalina
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
Álvarez, María E
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
  • Grupo de investigación Biosalud, Departamento de Ciencias Básicas para la Salud, Universidad de Caldas, Manizales, Colombia.
Pérez, Jorge E
  • Grupo de investigación Biosalud, Departamento de Ciencias Básicas para la Salud, Universidad de Caldas, Manizales, Colombia.
Bohórquez, Mabel E
  • Grupo de Investigación en Citogenética, Filogenia y Evolución de Poblaciones, Universidad del Tolima, Ibagué, Colombia.

MeSH Terms

  • Animals
  • Apoptosis / drug effects
  • Blood Platelets / metabolism
  • Cartilage / cytology
  • Cartilage / drug effects
  • Cartilage / metabolism
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / drug effects
  • Chondrocytes / metabolism
  • Culture Media, Conditioned / chemistry
  • Culture Media, Conditioned / pharmacology
  • Female
  • Gels / pharmacology
  • Gene Expression Regulation / drug effects
  • Horses
  • Hyaluronic Acid / analysis
  • Hyaluronic Acid / metabolism
  • Lipopolysaccharides / pharmacology

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