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Home / Equine Research Bank / Vet Surg / In vitro efficacy of a 0.2% polyhexamethylene biguanide-impr...
Veterinary surgery : VS2021; 50(4); 880-887; doi: 10.1111/vsu.13626

In vitro efficacy of a 0.2% polyhexamethylene biguanide-impregnated gauze dressing against pathogenic bacterial isolates found in horses.

Abstract: To determine the ability of 0.2% polyhexamethylene biguanide (PHMB)-impregnated gauze to inhibit the growth of bacteria isolated from equine infected sites. Methods: In vitro study. Methods: Nine bacterial isolates were obtained from cultures submitted from equine patients presenting with penetrating injuries of the hoof (n = 4), septic osteitis (n = 1), synovial sepsis (n = 1), wounds (n = 2), and incisional infection following laparotomy (n = 1). Two standardized strains were also included. A standard inoculum of each isolate was placed on 12 Muller-Hinton agar plates. Squares (2.5 cm × 2.5 cm) of 0.2% PHMB-impregnated (n = 6) and nonimpregnated control gauze (n = 6) were placed on inoculated agar plates. Bacterial growth under each gauze square was assessed after a 24-h incubation period and areas of inhibition were measured to a standardized scale, using image-processing software. Mean ± SD growth inhibition (%) using 0.2% PHMB-impregnated gauze was compared to the nonimpregnated gauze for each isolate using Student's t test (p < .05). Results: The 0.2% PMHB-impregnated gauze inhibited the growth of Staphylococcus spp. (n = 4) by 33%-83.1% and that of Escherichia coli spp. (n = 4) by 6.5%-37%. There was no inhibition of growth of Pseudomonas aeruginosa or either Enterococcus spp. Conclusions: The 0.2% PHMB-impregnated dressing tested here inhibited the growth of staphylococcal and E. coli isolates, but the magnitude of inhibition varied between strains. Conclusions: These results justify in vivo studies to evaluate the ability of the dressing to reduce the bacterial growth of common equine bacterial pathogens in clinical practice.
© 2021 American College of Veterinary Surgeons.
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Publication Date: 2021-03-30 PubMed ID: 33783851DOI: 10.1111/vsu.13626Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper investigates the effectiveness of a 0.2% polyhexamethylene biguanide (PHMB)-impregnated gauze in inhibiting bacterial growth in horses.

Objective of the Research

  • The primary objective of the research was to determine if a 0.2% PHMB-impregnated gauze could inhibit bacterial growth sourced from different infection sites in horses.

Methodology

  • A total of nine bacterial isolates from various equine infections was included in the study. The infections that the samples came from were hoof injuries, septic osteitis, synovial sepsis, wounds, and post-laparotomy incisional infections.
  • Two standardized strains were also incorporated in the assessment.
  • Each bacterial isolate was inoculated on 12 Muller-Hinton agar plates.
  • Squares of 0.2% PHMB-infused gauze and non-impregnated control gauze were placed on these agar plates.
  • Post 24-hour incubation, the bacterial growth under each square was assessed. The areas with inhibition of growth were measured using standardized software.
  • Effectiveness of the PHMB-impregnated gauze was determined based on the percentage of growth inhibition.

Results

  • The findings of the study revealed that the 0.2% PHMB-impregnated gauze was able to inhibit the growth of Staphylococcus spp. by 33%-83.1% and Escherichia coli spp. by 6.5%-37%.
  • No growth inhibition was observed in the case of Pseudomonas aeruginosa or any of the Enterococcus spp.
  • The degree of growth inhibition varied between different bacterial strains.

Conclusion

  • The PHMB-infused dressing demonstrated potential in inhibiting the growth of certain bacterial isolates like staphylococcal and E. coli; however, the degree of effectiveness varied between the strains.
  • The researchers suggested further in vivo studies to evaluate the effectiveness of the PHMB-infused dressing in clinical practice, considering these findings.

Cite This Article

APA
Noll CV, Kilcoyne I, Nieto JE, Thio T, Byrne BA. (2021). In vitro efficacy of a 0.2% polyhexamethylene biguanide-impregnated gauze dressing against pathogenic bacterial isolates found in horses. Vet Surg, 50(4), 880-887. https://doi.org/10.1111/vsu.13626

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 50
Issue: 4
Pages: 880-887

Researcher Affiliations

Noll, Charlene V
  • Department of Large Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, USA.
Kilcoyne, Isabelle
  • Department of Surgical and Radiological Sciences, University of California Davis, California, USA.
Nieto, Jorge E
  • Department of Surgical and Radiological Sciences, University of California Davis, California, USA.
Thio, Timothy
  • School of Veterinary Medicine, University of California at Davis, California, USA.
  • Penumbra Inc, One Penumbra Place, Alameda, California, USA.
Byrne, Barbara A
  • Department of Pathology, Microbiology and Immunology, University of California at Davis, California, USA.

MeSH Terms

  • Animals
  • Bandages / statistics & numerical data
  • Biguanides / pharmacology
  • Disinfectants / pharmacology
  • Escherichia coli / drug effects
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / prevention & control
  • Escherichia coli Infections / veterinary
  • Horse Diseases / microbiology
  • Horse Diseases / prevention & control
  • Horses
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / prevention & control
  • Staphylococcal Infections / veterinary
  • Staphylococcus / drug effects
  • Surgical Wound Infection / microbiology
  • Surgical Wound Infection / prevention & control
  • Surgical Wound Infection / veterinary

Grant Funding

  • Center for Equine Health, UC Davis

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Citations

This article has been cited 1 times.
  1. Loiko N, Kanunnikov O, Serdyukov D, Axelrod V, Tereshkin E, Vishnyakova A, Litti Y. Didecyldimethylammonium Chloride- and Polyhexamethylene Guanidine-Resistant Bacteria Isolated from Fecal Sludge and Their Potential Use in Biological Products for the Detoxification of Biocide-Contaminated Wastewater Prior to Conventional Biological Treatment. Biology (Basel) 2022 Sep 9;11(9).
    doi: 10.3390/biology11091332pubmed: 36138811google scholar: lookup
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