In vitro maturation and transfer of equine oocytes after transport of ovaries at 12 or 22 degrees C.
Abstract: Transportation of equine ovaries would allow shipment of oocytes for research purposes or transfer after the death of a valuable mare. The objective of this study was to compare two temperatures for maintaining ovaries during a transport interval of 18-24 h. The goal was to obtain pregnancies after transport of ovaries, maturation of oocytes in vitro, and transfer of oocytes. Each shipment was composed of ovaries four to seven mares collected from an abattoir. From each mare, one ovary was packaged at approximately 12 degrees C, and the other was packaged at approximately 22 degrees C. Upon arrival at our laboratory, oocytes were collected and cultured for 24 h. For each transfer, between 9 and 15 oocytes from each group were placed into the oviducts of estrous mares through standing flank laparotomies. Recipients received human chorionic gonadotropin (hCG; 2000 IU, i.v.) 30-36 h before transfer (to synchronize ovulation). Recipients were inseminated 18-20 h before transfers with 2 x 10(9) progressively motile sperm. Uteri of recipients were examined with ultrasound to determine the number of developing embryos. On Day 16 ( ovulation = day 0), developing embryos were recovered by uterine lavage. Parentage verification was performed on recovered vesicles. Pregnancy rates were analyzed by Chi-square. The percentage of oocytes that developed into embryonic vesicles on Day 16 was not different between transport temperatures (22 degrees C, 13/73, 18% versus 12 degrees C, 11/73, 15%). In conclusion, pregnancies were obtained from in vitro matured oocytes that were recovered from ovaries transported for 18-24h at 12 or 22 degrees C.
Publication Date: 2004-03-24 PubMed ID: 15036956DOI: 10.1016/j.theriogenology.2003.06.008Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study explores the viability of transporting equine ovaries for in vitro fertilisation. It compares the effectiveness of storing these ovaries at two different temperatures, 12 and 22 degrees Celsius, during transportation periods between 18 and 24 hours. The primary aim is to achieve successful pregnancies via the in vitro maturation and transfer of oocytes obtained from these transported ovaries.
Objective and Methodology
- The study aims to understand the viability of shipping equine ovaries — a critical aspect for equine breeding, research, and conservation purposes, specifically in cases involving the demise of a valuable mare.
- Part of the procedure involves subjecting the transported ovaries to in vitro maturation, followed by oocyte transfer to generate successful pregnancies.
- To realise these objectives, ovaries from 4–7 mares procured from an abattoir were packed at two different temperatures: around 12 degrees Celsius and 22 degrees Celsius.
- Upon receipt in the laboratory, oocytes harvested from these ovaries were cultured for 24 hours.
In Vitro Fertilisation
- For each transfer, 9–15 oocytes were placed into the oviducts of mares in estrus — a process executed through standing flank laparotomies.
- To ensure synchronised ovulation, the recipient mares were administered human chorionic gonadotropin (hCG; 2000 IU, i.v.) 30–36 hours before the transfer.
- Further, 18–20 hours before the transfers, the mares were inseminated with 2 x 10(9) progressively motile sperm.
Assessment and Results
- Post-transfer, the uteri of the recipient mares were examined using ultrasound to ascertain the number of developing embryos. On Day 16, embryonic vesicles were recovered.
- A Chi-square analysis revealed no significant difference in the percentage of oocytes developing into embryonic vesicles between the two transport temperatures.
- In conclusion, successful pregnancies were achieved from the in vitro maturation of oocytes drawn from ovaries shipped for 18–24 hours at both 12 degrees and 22 degrees Celsius.
Cite This Article
APA
Preis KA, Carnevale EM, Coutinho da Silva MA, Caracciolo di Brienza V, Gomes GM, Maclellan LJ, Squires EL.
(2004).
In vitro maturation and transfer of equine oocytes after transport of ovaries at 12 or 22 degrees C.
Theriogenology, 61(7-8), 1215-1223.
https://doi.org/10.1016/j.theriogenology.2003.06.008 Publication
Researcher Affiliations
- Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523, USA.
MeSH Terms
- Animals
- Female
- Horses
- Oocyte Donation / veterinary
- Oocytes / physiology
- Organ Preservation / veterinary
- Ovary / cytology
- Ovary / physiology
- Pregnancy
- Specimen Handling / methods
- Specimen Handling / veterinary
- Temperature
- Time Factors
Citations
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