In vitro predatory activity of nematophagous fungi and after passing through gastrointestinal tract of equine on infective larvae of Strongyloides westeri.
Abstract: Three isolates of predator fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34), and Arthrobotrys robusta (I-31) were assessed in in vitro test regarding the capacity of prey infective larvae (L(3)) Strongyloides westeri. Compared to control, without fungus, there was a significant decrease (P 0.01) in vitro test. Linear regression coefficients of treated and control groups were -0.21 for control, -0.32 for D. flagrans, -0.34 for M. thaumasium, and -0.22 for A. robusta. In the following, isolates AC001 and NF34 were assessed in vivo regarding the capacity of supporting the passage through equine gastrointestinal tract without loss of ability of preying infective larvae S. westeri. Fungal isolates survived the passage and were efficient in preying L(3) since the first 12 h of collection (P < 0.01) in relation to the control group (without fungus). Compared to control, there was a significant decrease (P < 0.01) of 76.4% and 76.7% (12 h), 86.4% and 85.9% (24 h), 88.3% and 87.7% (48 h), and 89.9% and 87.2% (72 h) in means of infective larvae S. westeri recovered from treatments with isolates AC001 and NF34, respectively. Linear regression coefficients of L(3) of recovered S. westeri regarding the collections due to time were 1.93 for control, -3.52 for AC001, and -2.64 for NF34. Fungi D. flagrans and M. thaumasium (NF34) have demonstrated to be promising for use in the biological control of equine parasite S. westeri.
Publication Date: 2010-04-06 PubMed ID: 20369256DOI: 10.1007/s00436-010-1841-yGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research evaluated the effectiveness of three predator fungi isolates (Duddingtonia flagrans, Monacrosporium thaumasium, and Arthrobotrys robusta) in reducing the quantity of infective larvae of the equine parasite Strongyloides westeri in controlled conditions. It was found that all three fungi were effective in prey infective larvae reduction and survived passage through the equine gastrointestinal tract, indicating potential use in the biological control of this equine parasite.
Overview of the Research
- The research studied the predatory activity of three different isolates of fungi (Duddingtonia flagrans, Monacrosporium thaumasium, and Arthrobotrys robusta) on the infective larvae of the equine parasite Strongyloides westeri.
- The primary aim was to observe their ability to reduce the concentration of the parasite in both controlled conditions and after passing through the equine gastrointestinal tract.
Research Methodology
- In the research, the in vitro test assessed the fungi’s ability to prey on the infective larvae (L(3)) of the S. westeri parasite.
- The results were compared against a control group without any fungus to assess the efficiency of different fungus isolates in consuming the parasite.
- Two of the most successful fungi isolates were further tested in vivo to understand their ability to survive the passage through the equine gastrointestinal tract without losing their predatory capacity.
Research Findings
- All three fungi isolates showed efficiency in capturing the S. westeri in the in vitro tests, and the fungal isolates AC001 and NF34 also demonstrated this efficiency in the in vivo tests.
- There was a significant decrease in the number of infective larvae recovered when compared to the control group, both in the in vitro tests and the in vivo tests after passing through the gastrointestinal tract.
- These results imply that the isolates Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) may show promise for usage in biological control of the S. westeri equine parasite. They are effective in prey on the parasite larvae and can survive passage through the equine gastrointestinal tract, maintaining their predatory capacity.
Cite This Article
APA
Araujo JM, Araújo JV, Braga FR, Carvalho RO.
(2010).
In vitro predatory activity of nematophagous fungi and after passing through gastrointestinal tract of equine on infective larvae of Strongyloides westeri.
Parasitol Res, 107(1), 103-108.
https://doi.org/10.1007/s00436-010-1841-y Publication
Researcher Affiliations
- Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Cep: 36570000, Brazil.
MeSH Terms
- Animals
- Ascomycota / isolation & purification
- Ascomycota / metabolism
- Ascomycota / pathogenicity
- Gastrointestinal Tract / microbiology
- Horses / microbiology
- Larva / microbiology
- Microbial Viability
- Pest Control, Biological / methods
- Strongyloides / microbiology
References
This article includes 14 references
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Citations
This article has been cited 2 times.- de Almeida GL, Santurio JM, Filho JO, Zanette RA, Camillo G, Flores AG, da Silva JH, de la Rue ML. Predatory activity of the fungus Duddingtonia flagrans in equine strongyle infective larvae on natural pasture in the Southern Region of Brazil.. Parasitol Res 2012 Feb;110(2):657-62.
- Ferreira SR, de Araújo JV, Braga FR, Araujo JM, Fernandes FM. In vitro predatory activity of nematophagous fungi Duddingtonia flagrans on infective larvae of Oesophagostomum spp. after passing through gastrointestinal tract of pigs.. Trop Anim Health Prod 2011 Dec;43(8):1589-93.
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