In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI.
Abstract: The magic angle effect increases the MRI signal of healthy tendon tissue and could be used for more detailed evaluation of tendon structure. Furthermore, it could support the discrimination of hypointense artefacts induced by contrast agents such as superparamagnetic iron oxide used for cell tracking. However, magic angle MRI of the equine superficial digital flexor tendon has not been accomplished in vivo in standing low-field MRI so far. The aim of this in vivo study was to evaluate the practicability of this magic angle technique and its benefit for tracking superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells. Six horses with induced tendinopathy in their forelimb superficial digital flexor tendons were injected locally either with superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells or serum. MRI included standard and magic angle image series in T1- and T2∗-weighted sequences performed at regular intervals. Image analysis comprised blinded evaluation and quantitative assessment of signal-to-noise ratio. The magic angle technique enhanced the tendon signal-to-noise ratio ( < 0.001). Hypointense artefacts were observable in the cell-injected superficial digital flexor tendons over 24 weeks and artefact signal-to-noise ratio differed significantly from tendon signal-to-noise ratio in the magic angle images ( < 0.001). Magic angle imaging of the equine superficial digital flexor tendon is feasible in standing low-field MRI. The current data demonstrate that the technique improves discrimination of superparamagnetic iron oxide-induced artefacts from the surrounding tendon tissue.
Copyright © 2019 Carolin Horstmeier et al.
Publication Date: 2019-12-17 PubMed ID: 31933650PubMed Central: PMC6942896DOI: 10.1155/2019/5670106Google Scholar: Lookup
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- Journal Article
- Bioinformatics
- Biotechnology
- Cell Culture
- Cell Proliferation
- Clinical Study
- Diagnostic Imaging
- Equine Diseases
- Equine Health
- Equine Science
- Experimental Methods
- Imaging Techniques
- In Vivo
- Magnetic Resonance Imaging
- Mesenchymal Cells
- Musculoskeletal System
- Stem Cells
- Superficial Digital Flexor Tendon
- Tendons
- Veterinary Medicine
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the use of ‘magic angle’ magnetic resonance imaging (MRI) in tracking mesenchymal stromal cells in horses. By enhancing the contrast of the images, they were able to more accurately track and evaluate the injection of regenerative cells into horse tendons.
What is Magic Angle MRI?
- Magic angle MRI is a technique that can increase the MRI signal of the healthy tendon tissue. This can enable more detailed evaluation of the tendon structure.
- The magic angle effect can help differentiate between the healthy tissue and artefacts that are induced by certain contrast agents, in this case, the superparamagnetic iron oxide used for cell tracking.
Purpose of the Study
- This study aimed to assess the practicality of using the magic angle technique in in vivo tracking of superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells in horses.
- The study focuses on the superficial digital flexor tendon in horses’ forelimbs since this particular tendon has not been exposed to the magic angle MRI technique in standing low-field MRI.
Method and Results
- The study was conducted on six horses with induced tendinopathy. The tendons were locally injected with either superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells or serum.
- Both standard and magic angle image series in T1- and T2∗-weighted sequences were performed at regular intervals.
- Images were analysed qualitatively and quantitatively, with the focus on the signal-to-noise ratio.
- Findings show that the magic angle technique significantly increased the tendon signal-to-noise ratio and improved the visibility of hypointense artefacts in the cell-injected tendons, observable over 24 weeks. The artefact signal-to-noise ratio significantly differed from the tendon signal-to-noise ratio in magic angle images.
Conclusion
- The research concludes that magic angle imaging of the equine superficial digital flexor tendon is achievable in standing, low-field MRI.
- The data demonstrate that the technique significantly improves the discrimination of superparamagnetic iron oxide-induced artefacts from the surrounding tendon tissue, hence enhancing the accuracy and reliability of cell tracking.
Cite This Article
APA
Horstmeier C, Ahrberg AB, Berner D, Burk J, Gittel C, Hillmann A, Offhaus J, Brehm W.
(2019).
In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI.
Stem Cells Int, 2019, 5670106.
https://doi.org/10.1155/2019/5670106 Publication
Researcher Affiliations
- Department for Horses, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany.
- Department of Orthopedics, Traumatology, and Plastic Surgery, University of Leipzig, Liebigstr. 20, 04103 Leipzig, Germany.
- Royal Veterinary College, University of London, Hawkshead Lane, Hatfield, Hertfordshire AL9 7TA, UK.
- Equine Clinic-Surgery, Justus Liebig University Giessen, Frankfurter Str. 108, 35392 Giessen, Germany.
- Department of Veterinary Medicine, Queen's Veterinary School, Madingley Road, Cambridge CB3 0ES, UK.
- Saxon Incubator for Clinical Translation, University of Leipzig, Philipp-Rosenthal-Str. 55, 04103 Leipzig, Germany.
- Department for Horses, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany.
- Department for Horses, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany.
- Saxon Incubator for Clinical Translation, University of Leipzig, Philipp-Rosenthal-Str. 55, 04103 Leipzig, Germany.
Conflict of Interest Statement
Each author certifies that he or she has no commercial associations that might pose a conflict of interest in connection with the submitted article.
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Citations
This article has been cited 1 times.- Doll CU, Bohner M, Berner D, Buettner K, Horstmeier C, Winter K, Burk J. Approaches to standardising the magnetic resonance image analysis of equine tendon lesions.. Vet Rec Open 2023 Jun;10(1):e257.
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