Increased concentrations of protein gene product 9.5 in the synovial fluid from horses with osteoarthritis.
Abstract: Our previous study established protein gene product 9.5 (PGP 9.5), a ubiquitin C-terminal hydrolase, as a specific cytochemical marker of synovial lining cells (type B synoviocytes) in the horse joint. The present study aimed to detect PGP 9.5 in the synovial fluid and shows that PGP 9.5 is a valuable marker of osteoarthritis in the horse. Immunohistochemical staining confirmed rich and consistent localization of PGP 9.5 immunoreactivity in the cytoplasm of synovial lining cells in the normal horse joint. Western blot analysis of synovial fluid from normal joints could detect a significant band corresponding to that contained in the brain and synovial membrane extracts. When 60 synovial fluid samples from normal and abnormal joints were assayed with an enzyme-linked immunosorbent assay (ELISA) system, the concentration of PGP 9.5 tended to be elevated in osteochondrosis dissecance, inflammatory arthropathy and intra-articular fracture, among which a statistically significant elevation was recognizable between the intra-articular fracture and the control. Thus, this study demonstrated the possibility that PGP 9.5, derived from synovial lining cells, may be a new biochemical marker for arthritic disorders of the horse.
Publication Date: 2001-10-10 PubMed ID: 11590919
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research explains that the protein gene product 9.5 (PGP 9.5), which is found in the synovial fluid of horses, can be used as a marker for osteoarthritis in the species. The study further details that the levels of PGP 9.5 were noticeably increased in horses suffering from osteoarthritis.
Identification and Role of Protein Gene Product 9.5 (PGP 9.5)
- The research first disclosed that PGP 9.5 was identified as a specific cytochemical marker of synovial lining cells, termed as type B synoviocytes, in the horse joint.
- PGP 9.5 is a ubiquitin C-terminal hydrolase, an enzyme that has a significant role in the ubiquitin-proteasome pathway. This pathway is crucial in the degradation of intracellular proteins, which helps in maintaining cellular homeostasis.
Detecting PGP 9.5 and its Localization
- The researchers used immunohistochemical staining, a technique that uses specific antibodies to detect the presence of proteins, to find the localization of PGP 9.5.
- It was found to be rich and uniformly present in the cytoplasm of the synovial lining cells of a normal horse joint. This suggests that PGP 9.5 is a naturally occurring substance in horse joints.
- Western blot analysis further confirmed the presence of PGP 9.5 in synovial fluid from healthy joints.
Role of PGP 9.5 Level in Osteoarthritis
- The study proceeded to measure the concentration of PGP 9.5 in 60 synovial fluid samples from horse joints, both normal and abnormal, using the enzyme-linked immunosorbent assay (ELISA) system.
- The results revealed an increased concentration of PGP 9.5 in instances such as osteochondrosis dissecance, inflammatory arthropathy and intra-articular fracture, conditions usually associated with osteoarthritis.
- Specifically, a significantly notable elevation was observed between those diagnosed with intra-articular fracture and the control samples.
Conclusion
- The findings from this study hinted at the possibility that PGP 9.5, which is derived from the horses’ synovial lining cells, could serve as a new viable biochemical marker for detecting arthritic disorders like osteoarthritis.
- By monitoring the levels of PGP 9.5, early detection of osteoarthritis could be possible, leading to timely treatment of the condition in horses.
Cite This Article
APA
Kitamura H, Okumura M, Sato F, Kimoto K, Kohama M, Hashimoto Y, Tagami M, Iwanaga T.
(2001).
Increased concentrations of protein gene product 9.5 in the synovial fluid from horses with osteoarthritis.
Jpn J Vet Res, 49(2), 115-123.
Publication
Researcher Affiliations
- Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818.
MeSH Terms
- Animals
- Blotting, Western / veterinary
- Enzyme-Linked Immunosorbent Assay / veterinary
- Female
- Horse Diseases / metabolism
- Horses
- Immunohistochemistry / veterinary
- Osteoarthritis / diagnosis
- Osteoarthritis / veterinary
- Synovial Fluid / metabolism
- Thiolester Hydrolases / metabolism
- Ubiquitin Thiolesterase
Citations
This article has been cited 3 times.- Emmi A, Stocco E, Boscolo-Berto R, Contran M, Belluzzi E, Favero M, Ramonda R, Porzionato A, Ruggieri P, De Caro R, Macchi V. Infrapatellar Fat Pad-Synovial Membrane Anatomo-Fuctional Unit: Microscopic Basis for Piezo1/2 Mechanosensors Involvement in Osteoarthritis Pain. Front Cell Dev Biol 2022;10:886604.
- Imamura F, Greer CA. Segregated labeling of olfactory bulb projection neurons based on their birthdates. Eur J Neurosci 2015 Jan;41(2):147-56.
- Kraus VB, Hsueh MF. Molecular biomarker approaches to prevention of post-traumatic osteoarthritis. Nat Rev Rheumatol 2024 May;20(5):272-289.
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