Indigenous West Nile virus infections in horses in Albania.
Abstract: Serum samples collected from 167 equines of 12 districts in Albania were tested for West Nile virus-specific antibodies by enzyme-linked immunosorbent assay and virus neutralization assay, using WNV lineage 1 and 2. In addition, 95 bird serum samples from Albania and 29 horse samples from Kosovo were tested in ELISA. An overall seroprevalence rate of 22% was found in horses from Albania, whereas no specific antibodies were found in the equine samples from Kosovo and the bird samples. This is the first report indicating WNV infections in animals in Albania, and the first reported seroprevalence study conducted for Kosovo. These results provide evidence for widespread infections of WNV in Albania.
© 2013 Blackwell Verlag GmbH.
Publication Date: 2014-03-05 PubMed ID: 24589101DOI: 10.1111/tbed.12141Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper discusses the detection of the West Nile Virus (WNV) in horses from various districts in Albania, with a significant prevalence rate, as well as the absence of the WNV in horses and birds examined in Kosovo.
Methodology & Samples
- The research used samples acquired from 167 horses across 12 districts in Albania. In addition to this primary set of samples, 95 bird serum samples from Albania and 29 horse samples from Kosovo were also used.
- The study employed techniques like enzyme-linked immunosorbent assay (ELISA) and virus neutralization assays. These techniques can detect the presence of WNV-specific antibodies in the specimens, which signify an immune response to a past or recent infection with the virus.
Findings from Albania
- The result of the Albanian samples indicated a 22% seroprevalence rate amongst horses, marking the presence of WNV in these horses. Seroprevalence refers to the total number of individuals in a population who test positive for a specific disease based on serology (blood serum) specimens. This means approximately 22% of horses sampled in Albania had WNV-specific antibodies, indicating prior exposure to the infection.
Findings from Kosovo
- In contrast, no specific WNV antibodies were discovered within samples gathered from both Kosovo’s horses or birds. This absence suggests that, these animals had not been exposed to the West Nile Virus.
Significance of the Study
- This study is crucial as it is the first of its kind to indicate WNV infection among animals in Albania, and it provided the first-ever seroprevalence report from Kosovo.
- The evidence of widespread WNV in Albania underscores potential public health risks given that the virus can be transmitted to humans via the bites of infected mosquitoes. Preventive measures to control the disease’s transmission could thus be necessary.
Cite This Article
APA
Berxholi K, Ziegler U, Rexhepi A, Schmidt K, Mertens M, Korro K, Cuko A, Angenvoort J, Groschup MH.
(2014).
Indigenous West Nile virus infections in horses in Albania.
Transbound Emerg Dis, 60 Suppl 2, 45-50.
https://doi.org/10.1111/tbed.12141 Publication
Researcher Affiliations
- Faculty of Veterinary Medicine in Tirana, Tirana, Albania.
MeSH Terms
- Albania / epidemiology
- Animals
- Antibodies, Viral / blood
- Antibodies, Viral / immunology
- Enzyme-Linked Immunosorbent Assay
- Horse Diseases / epidemiology
- Horse Diseases / transmission
- Horse Diseases / virology
- Horses / virology
- Seroepidemiologic Studies
- West Nile Fever / epidemiology
- West Nile Fever / veterinary
- West Nile Fever / virology
- West Nile virus / immunology
Citations
This article has been cited 3 times.- Gothe LMR, Ganzenberg S, Ziegler U, Obiegala A, Lohmann KL, Sieg M, Vahlenkamp TW, Groschup MH, Hörügel U, Pfeffer M. Horses as Sentinels for the Circulation of Flaviviruses in Eastern-Central Germany.. Viruses 2023 Apr 30;15(5).
- Vilibic-Cavlek T, Savic V, Petrovic T, Toplak I, Barbic L, Petric D, Tabain I, Hrnjakovic-Cvjetkovic I, Bogdanic M, Klobucar A, Mrzljak A, Stevanovic V, Dinjar-Kujundzic P, Radmanic L, Monaco F, Listes E, Savini G. Emerging Trends in the Epidemiology of West Nile and Usutu Virus Infections in Southern Europe.. Front Vet Sci 2019;6:437.
- Di Sabatino D, Bruno R, Sauro F, Danzetta ML, Cito F, Iannetti S, Narcisi V, De Massis F, Calistri P. Epidemiology of West Nile disease in Europe and in the Mediterranean Basin from 2009 to 2013.. Biomed Res Int 2014;2014:907852.
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