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Journal of Zhejiang University. Science. B2015; 16(12); 980-990; doi: 10.1631/jzus.B1500046

Induction of follicular luteinization by equine chorionic gonadotropin in cyclic guinea pigs.

Abstract: The effects of equine chorionic gonadotropin (eCG) on follicular development and ovulation in cyclic guinea pigs were investigated by histological and immunohistochemical analyses. Three groups of guinea pigs (n=12) were administrated subcutaneously with saline, 20 or 50 IU of eCG, respectively, on cyclic Day 12 (Day 1=vaginal openings). Ovaries were collected at 4 and 8 d after administration (6 animals per group each time). The eCG administration induced significant and distinct morphological changes in the ovaries, as it promoted the luteinization of granulosa cells, but not follicular development. In addition, proliferating cell nuclear antigen (PCNA) and steroidogenic acute regulatory protein (StAR) were immunolocalized specifically in luteinized follicles. Our experiments together indicate that eCG administration can induce follicular luteinization but not superovulation in guinea pigs. The eCG in cyclic guinea pigs functions similar to that of luteinizing hormone (LH), but not follicle-stimulating hormone (FSH).
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Publication Date: 2015-12-08 PubMed ID: 26642181PubMed Central: PMC4678041DOI: 10.1631/jzus.B1500046Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the impact of equine chorionic gonadotropin (eCG) on ovarian development and ovulation in cyclic guinea pigs, determining that eCG can trigger changes in ovaries similar to luteinizing hormone but doesn’t stimulate overproduction of eggs.

Research Objectives

  • This research was conducted to observe the effects of Equine Chorionic Gonadotropin (eCG) on ovary development and ovulation in cyclic guinea pigs.
  • It aimed to understand the similarities and differences of eCG with other female hormones, specifically luteinizing hormone (LH) and follicle-stimulating hormone (FSH).

Methods

  • Three groups of guinea pigs were administered subcutaneously (under the skin) with three different solutions: saline, 20 IU, or 50 IU of eCG respectively. It was given on the 12th day of their reproductive cycle where Day 1 is considered the day of vaginal opening.
  • The ovaries were then collected at 4 and 8 days after the administration for determining the effects of the eCG on the development of the ovaries.

Findings

  • It was observed that the administration of the eCG led to significant morphological changes in the ovaries of the guinea pigs.
  • These changes included the promotion of luteinization of granulosa cells, which surround all stages of the developing immature eggs (oocytes) in the ovaries but did not prompt follicular development or superovulation (production and release of multiple eggs).
  • Furthermore, two specific proteins proliferating cell nuclear antigen (PCNA) and steroidogenic acute regulatory protein (StAR), were present specifically in the luteinized follicles in large amounts. PCNA is a marker for dividing cells and StAR is a protein involved in the synthesis of steroids including estrogen and progesterone.
  • This led to the conclusion that eCG administration in cyclic guinea pigs triggered follicular luteinization but not superovulation, indicating it functions similarly to LH, but not to FSH.

Implications

  • The research could potentially be used to understand the effects and functioning of eCG and its use in the field of animal or even human reproduction.
  • The insights gained from this research might help in manipulating and controlling certain aspects of the reproductive process in guinea pigs and may have implications for other mammals.

Cite This Article

APA
Li JR, Wang W, Shi FX. (2015). Induction of follicular luteinization by equine chorionic gonadotropin in cyclic guinea pigs. J Zhejiang Univ Sci B, 16(12), 980-990. https://doi.org/10.1631/jzus.B1500046

Publication

Journal of Zhejiang University. Science. B
ISSN: 1862-1783
NlmUniqueID: 101236535
Country: China
Language: English
Volume: 16
Issue: 12
Pages: 980-990

Researcher Affiliations

Li, Jun-rong
  • Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
  • College of Agriculture and Bio-engineering, Jinhua Polytechnic, Jinhua 321017, China.
Wang, Wei
  • Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Shi, Fang-xiong
  • Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

MeSH Terms

  • Animals
  • Apoptosis / drug effects
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Follicular Atresia / drug effects
  • Gonadotropins, Equine / administration & dosage
  • Guinea Pigs
  • Immunohistochemistry
  • Luteinization / drug effects
  • Luteinization / physiology
  • Oocytes / cytology
  • Oocytes / drug effects
  • Ovarian Follicle / cytology
  • Ovarian Follicle / drug effects
  • Ovarian Follicle / physiology
  • Phosphoproteins / metabolism
  • Proliferating Cell Nuclear Antigen / metabolism
  • Superovulation / drug effects

Conflict of Interest Statement

Jun-rong LI, Wei WANG, and Fang-xiong SHI declare that they have no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.

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Citations

This article has been cited 1 times.
  1. Wang J, Liu Z, Sun Q, Xia S, Cui J, Yang L, An L, Zhang J, Su L, Su Y, Du F. Combined treatment with cysteamine and leukemia inhibitory factor promotes guinea pig oocyte meiosis in vitro. Am J Transl Res 2019;11(12):7479-7491.
    pubmed: 31934295
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