Induction of heat shock protein 72 mRNA in skeletal muscle by exercise and training.
Abstract: In response to stress, cells synthesise heat shock proteins (HSP) to maintain protein homeostasis. To study whether exercise and training induce expression of HSP72 in the middle gluteal muscle, 10 Finnhorses performed a submaximal 60 min exercise test on a treadmill. Test A was performed after 3 months of training, and the other two tests 2 (B) and 5 (C) weeks later. Blood samples were taken during and after the tests, and biopsy samples before, immediately after and 23 h after each test. HSP72 mRNA was analysed using a digoxigenin-labelled probe. Blood lactate concentration in the 3 tests varied between 7.2 and 10.2 mmol/l. Training increased HSP72 mRNA, as indicated by increases in samples taken at rest (A<B<C). Exercise also tended to increase HSP72 mRNA transiently but, 23 h later, values had returned to pre-exercise levels. HSP72 mRNA was expressed in all muscle fibres. After exercise, HSP72 mRNA correlated positively with the peak concentration of blood lactate, but not with indicators of energy status. Therefore, acidosis rather than energy depletion was the major inducer of HSP72 expression after moderate intensity exercise. Because HSP72 may protect cells against stress, knowledge about their expression may help in planning optimal trainng regimes.
Publication Date: 2002-10-31 PubMed ID: 12405689DOI: 10.1111/j.2042-3306.2002.tb05421.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research article explores the impact of exercise and training on the expression of heat shock protein 72 (HSP72) mRNA in skeletal muscles of horses. The results suggest both exercise and training stimulate the production of HSP72 in muscles, and this protein expression may play a significant role in managing stress in muscle cells.
Experimental Procedure
- The experiment involved ten Finnhorses, and their middle gluteal muscle was put under study. The horses underwent a submaximal 60-minute exercise test on a treadmill.
- The experiment had three parts – the initial test referred to as Test A was carried out after three months of training. Subsequent tests (B and C) were performed 2 weeks and 5 weeks later, respectively.
- Data collection was done through blood and biopsy samples. The samples were taken at different time intervals – during and post the fitness tests, before, right after, and 23 hours after each test.
Analysis and Results
- The heat shock protein 72 mRNA’s presence and expression were analyzed using a technique involving a digoxigenin-labeled probe. HSP72 mRNA was found in all muscle fibers.
- Training was observed to enhance the expression of HSP72 mRNA, demonstrated by an incremental pattern in samples taken when the horses were at rest (A<B<C).
- A temporary increase in HSP72 mRNA was noticed post-exercise, but the levels returned to pre-exercise values 23 hours later.
- The analysis showed that blood lactate concentration in the trials varied between 7.2 and 10.2 mmol/l, meaning acidity levels in the blood rather than energy depletion majorly triggered the expression of HSP72 mRNA post-exercise. However, there was no correlation with indicators of energy status.
Conclusion and Implications
- With the documented role of HSP72 in protecting cells against stress, understanding their expression could potentially aid in creating optimal training regimes for performance animals.
- Thus, the study successfully demonstrated that moderate-intensity exercise resulted in an increase in HSP72 mRNA levels, implying a cellular stress response.
Cite This Article
APA
Pösö AR, Eklund-Uusitalo S, Hyyppä S, Pirilä E.
(2002).
Induction of heat shock protein 72 mRNA in skeletal muscle by exercise and training.
Equine Vet J Suppl(34), 214-218.
https://doi.org/10.1111/j.2042-3306.2002.tb05421.x Publication
Researcher Affiliations
- Department of Basic Veterinary Sciences, University of Helsinki, Finland.
MeSH Terms
- Animals
- Biopsy, Needle / veterinary
- Exercise Test / veterinary
- Gene Expression Regulation / physiology
- HSP72 Heat-Shock Proteins
- Heat-Shock Proteins / biosynthesis
- Heat-Shock Proteins / genetics
- Horses / metabolism
- Horses / physiology
- Lactic Acid / blood
- Muscle, Skeletal / metabolism
- Physical Conditioning, Animal / physiology
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
- Time Factors
Citations
This article has been cited 6 times.- Ebisuda Y, Mukai K, Takahashi Y, Yoshida T, Kawano A, Matsuhashi T, Miyata H, Kuwahara M, Ohmura H. Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle.. Front Vet Sci 2023;10:1230212.
- Stary CM, Hogan MC. Cytosolic calcium transients are a determinant of contraction-induced HSP72 transcription in single skeletal muscle fibers.. J Appl Physiol (1985) 2016 May 15;120(10):1260-6.
- Smuder AJ, Kavazis AN, Min K, Powers SK. Exercise protects against doxorubicin-induced oxidative stress and proteolysis in skeletal muscle.. J Appl Physiol (1985) 2011 Apr;110(4):935-42.
- Stary CM, Walsh BJ, Knapp AE, Brafman D, Hogan MC. Elevation in heat shock protein 72 mRNA following contractions in isolated single skeletal muscle fibers.. Am J Physiol Regul Integr Comp Physiol 2008 Aug;295(2):R642-8.
- Gjøvaag TF, Dahl HA. Effect of training and detraining on the expression of heat shock proteins in m. triceps brachii of untrained males and females.. Eur J Appl Physiol 2006 Oct;98(3):310-22.
- Kinnunen S, Hyyppä S, Lappalainen J, Oksala N, Venojärvi M, Nakao C, Hänninen O, Sen CK, Atalay M. Exercise-induced oxidative stress and muscle stress protein responses in trotters.. Eur J Appl Physiol 2005 Jan;93(4):496-501.
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