Inflammatory markers in bronchoalveolar lavage fluid of standardbred racehorses with inflammatory airway disease: response to interferon-alpha.
Abstract: Protein and eicosanoid concentrations and procoagulant activity were determined in bronchoalveolar lavage fluid (BALF) from 32 Standardbred racehorses with inflammatory airway disease (IAD) and 6 control horses. Total protein, albumin and immunoglobulin G (IgG) concentrations were high (P < 0.05) in the BALF from horses with IAD, a finding consistent with exudation of plasma protein into the airway. Immunoglobulin A (IgA) concentrations also were increased (P < 0.05) which may signify local immunoglobulin production. Difference was not detected in prostaglandin E2 and 6-ketoprostaglandin F1 alpha concentrations in BALF of IAD-affected and control horses. Procoagulant activity was identified in the majority (66%) of BALF samples from IAD-affected horses and was not detected in control horses. Natural human interferon-alpha (nHulFN alpha) (placebo, 50, 150, or 450 units) was administered orally for 5 days to IAD-affected horses in a double-blind, randomised block design. Total protein, IgG, and IgA concentrations in BALF were reduced (P < 0.05) 8 days after administration of 50 u and 150 u nHuIFN alpha, and 15 days after administration of 50 u nHuIFN alpha. Procoagulant activity and albumin concentrations in BALF were lower 8 days after administration of 50 u nHuIFN alpha. Oral administration of low-dose nHuIFN alpha appeared to ameliorate these parameters of lower respiratory tract inflammation in Standardbred racehorses with IAD.
Publication Date: 1997-03-01 PubMed ID: 9104564DOI: 10.1111/j.2042-3306.1997.tb01656.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Clinical Trial
- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study examines inflammatory markers in standardbred racehorses suffering from inflammatory airway disease (IAD), and the effect of interferon-alpha treatment on those markers.
Objective of the study
- The objective of this research was to observe the concentrations of proteins, eicosanoids, and procoagulants in the bronchoalveolar lavage fluid (BALF) of 32 racehorses suffering from inflammatory airway disease (IAD) and compare it with 6 healthy horses. The study also aimed to determine the effects of natural human interferon-alpha (nHuIFN alpha), administered orally at different doses, on these inflammatory markers.
Findings from the study
- It was found that total protein, albumin, and immunoglobulin G (IgG) concentrations were considerably higher in IAD-affected horses compared to control horses. This suggests that plasma proteins might be leaking into the airway due to the inflammation.
- An increase in Immunoglobulin A (IgA) concentrations was observed in IAD-affected horses, which could indicate local immunoglobulin production.
- However, there was no significant difference in the concentrations of prostaglandin E2 and 6-ketoprostaglandin F1 alpha (types of eicosanoids) between the IAD-affected and control horses.
- The study also revealed that procoagulant activity was present in 66% of BALF samples from IAD-affected horses, but was not detected in control horses. This could indicate a role of coagulation in IAD condition.
Impact of nHuIFN alpha treatment
- nHuIFN alpha was orally administered to horses with IAD, in different dosages (placebo, 50, 150, or 450 units) for 5 consecutive days. This design was followed in a double-blind, randomized block manner.
- It was observed that total protein, IgG, and IgA concentrations in BALF decreased 8 days after the administration of 50 u and 150 u nHuIFN alpha, and 15 days after the administration of 50 u nHuIFN alpha.
- Procoagulant activity and albumin concentrations in BALF also seemed to be lowered around 8 days after the administration of 50 u nHuIFN alpha.
- The study concluded that low-dose oral administration of nHuIFN alpha appeared to ease these markers of lower respiratory tract inflammation in Standardbred racehorses with IAD, hence showing potential for being an effective treatment strategy.
Cite This Article
APA
Moore BR, Krakowka S, Mcvey DS, Cummins JM, Robertson JT.
(1997).
Inflammatory markers in bronchoalveolar lavage fluid of standardbred racehorses with inflammatory airway disease: response to interferon-alpha.
Equine Vet J, 29(2), 142-147.
https://doi.org/10.1111/j.2042-3306.1997.tb01656.x Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan 66506-5606, USA.
MeSH Terms
- 6-Ketoprostaglandin F1 alpha / analysis
- 6-Ketoprostaglandin F1 alpha / metabolism
- Administration, Oral
- Albumins / analysis
- Albumins / metabolism
- Animals
- Antiviral Agents / administration & dosage
- Antiviral Agents / therapeutic use
- Bronchoalveolar Lavage Fluid / chemistry
- Dinoprostone / analysis
- Dinoprostone / metabolism
- Dose-Response Relationship, Drug
- Double-Blind Method
- Eicosanoids / analysis
- Female
- Horse Diseases / drug therapy
- Horse Diseases / metabolism
- Horses
- Humans
- Immunoglobulin A / analysis
- Immunoglobulin A / metabolism
- Immunoglobulin G / analysis
- Immunoglobulin G / metabolism
- Interferon-alpha / administration & dosage
- Interferon-alpha / therapeutic use
- Male
- Proteins / analysis
- Proteins / metabolism
- Respiratory Tract Diseases / drug therapy
- Respiratory Tract Diseases / metabolism
- Respiratory Tract Diseases / veterinary
- Sports
- Time Factors
Citations
This article has been cited 2 times.- Couëtil LL, Cardwell JM, Gerber V, Lavoie JP, Léguillette R, Richard EA. Inflammatory Airway Disease of Horses--Revised Consensus Statement. J Vet Intern Med 2016 Mar-Apr;30(2):503-15.
- Moore I, Horney B, Day K, Lofstedt J, Cribb AE. Treatment of inflammatory airway disease in young standardbreds with interferon alpha. Can Vet J 2004 Jul;45(7):594-601.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
