Inflammatory response following intra-articular injection of decellularized porcine amnion/chorion in horses.
Abstract: The objective of the study was to evaluate effects of intra-articular decellularized porcine amnion/chorion suspension (dPACLS) in horses. Methods: Controlled, randomized, prospective study. Methods: A total of 10 adult horses. Methods: One randomly selected radiocarpal joint (RCJ) was injected with a high dose (HD) of 50 mg (n = 5) or low dose (LD) of 5 mg of dPACLS (n = 5). The contralateral RCJ received saline as control. Systemic serum amyloid A (SAA), synovial fluid analysis (including SAA) and subjective lameness evaluation were performed prior to injection (time 0) and 24, 48, 72, 96, 168 h post-injection. Results: Synovial fluid nucleated cell count (NCC) in both groups was higher than controls at 24 and 48 h post-injection. The HD group had significantly higher synovial fluid SAA values than the LD group and the controls. Systemic SAA was higher in HD group than baseline at all timepoints except 168 h, but no difference was detected from baseline in LD group at any timepoint. Systemic SAA was significantly greater in the HD group than the LD group at all timepoints except at baseline and 168 h. Three HD horses were lame and had elevated NCC at 168 h. Conclusions: The HD group had more clinically evident inflammation in comparison to the LD group based on systemic and synovial SAA, subjective lameness scores, and carpal flexion. Conclusions: Intra-articular injection of dPACLS at the doses studied is not recommended for intra-articular injection due to the inflammatory response.
© 2025 American College of Veterinary Surgeons.
Publication Date: 2025-08-25 PubMed ID: 40852903DOI: 10.1111/vsu.70005Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
- Veterinary
Cite This Article
APA
Cormier KV, Fontenot RL, Eddy A, Williams ML, Wills RW, Jumper WI, Mochal-King CA.
(2025).
Inflammatory response following intra-articular injection of decellularized porcine amnion/chorion in horses.
Vet Surg, 54(7), 1454-1462.
https://doi.org/10.1111/vsu.70005 Publication
Researcher Affiliations
- Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA.
- Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA.
- Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA.
- Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA.
- Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA.
- Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA.
- Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA.
MeSH Terms
- Animals
- Horses
- Injections, Intra-Articular / veterinary
- Injections, Intra-Articular / adverse effects
- Synovial Fluid / cytology
- Synovial Fluid / chemistry
- Female
- Amnion / transplantation
- Swine
- Horse Diseases
- Prospective Studies
- Lameness, Animal
- Inflammation / veterinary
- Inflammation / etiology
- Male
- Serum Amyloid A Protein / analysis
Grant Funding
- Triad Life Science
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