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Journal of equine veterinary science2019; 79; 105-112; doi: 10.1016/j.jevs.2019.05.023

Influence of Dietary Supplementation of Ensiled Devil Fish and Staphylococcus saprophyticus on Equine Fecal Greenhouse Gases Production.

Abstract: The present context was designed to investigate the efficacy of devil fish (DF; Plecostomus sp.) silage and Staphylococcus saprophyticus on fermentation characteristics as well as greenhouse gases production mitigation attributes in horses. Four levels of ensiled DF at 0 (control DF0), 6 (DF6), 12 (DF12), and 18 (DF18) % were added into the diet. Moreover, three doses of S. saprophyticus (0, 1, and 3 mL/g dry matter [DM]) were used for in vitro fecal fermentation. The use of ensiled DF resulted in increased (P < .0001) pH during fermentation. The asymptotic gas production was the highest (P < .0001) in DF6, whereas other supplementation caused lower production than that of control. Lag time for the asymptotic gas production decreased (P < .05) with increasing dietary DF doses. Inclusion of S. saprophyticus resulted in the lowest (P < .05) gas production and mL/0.5 g DM incubated and thus, the reduced gas production up to 23.17% than that of control. The interaction of DF × S. saprophyticus showed the lowest gas production at DF18, whereas the highest production was estimated at DF6 without S. saprophyticus after 48 hours. The lowest emission of CO2 (P < .0001) was observed in DF18 inclusion, which was 15.25% lower than that of control at 48 hours of fermentation. In contrast, the lowest hydrogen (H2) production was estimated in DF0, whereas DF18 exhibited the highest. Inclusion of DF12 and DF18 reduced (P < .05) methane (CH4) emission by 58.24% and 59.33%, respectively. However, DF, S. saprophyticus, and DF × S. saprophyticus interaction had no significant effect (P > .05) on CH4 production. In conclusion, ensiled DF and S. saprophyticus could be supplemented in equine diet as promising alternatives to corn for mitigating the emission of greenhouse gases effectively.
Publication Date: 2019-06-11 PubMed ID: 31405488DOI: 10.1016/j.jevs.2019.05.023Google Scholar: Lookup
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  • Journal Article

Summary

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The study investigated the impact of incorporating devil fish and Staphylococcus saprophyticus in horse diets on fermentation attributes and greenhouse gases production. The researchers observed reduced greenhouse gas emissions, suggesting that these dietary supplements hold promise as alternatives to corn.

Context and Purpose

  • The research was designed to evaluate the effectiveness of including dietary doses of devil fish (Plecostomus sp.) and Staphylococcus saprophyticus in the diets of horses.
  • The target parameters were fermentation characteristics and the potential in reducing the production of greenhouse gases.

Methodology

  • The study involved using four levels of devil fish: 0 (control), 6, 12, and 18 percent.
  • Also incorporated were three doses of Staphylococcus saprophyticus.
  • Involved trials on in vitro fecal fermentation, allowing the researchers to monitor gas production and analyze other fermentation criteria such as pH value.

Key Findings

  • The study found that the inclusion of devil fish led to an increased pH during fermentation.
  • To a certain limit (6 percent), it was observed that inclusion of devil fish increased the asymptotic gas production, whereas higher doses resulted in lower production.
  • Incorporation of Staphylococcus saprophyticus resulted in the lowest gas production, reducing gas emission by up to almost 23.17 percent.
  • Gas production was also influenced by the interaction of devil fish and Staphylococcus saprophyticus. The highest gas was produced with 6 percent devil fish without Staphylococcus saprophyticus, while the lowest was produced with 18 percent devil fish.
  • Carbon monoxide emissions were lowest with 18 percent devil fish. The emission of carbon monoxide dropped by 15.25 percent compared to the control group without devil fish.
  • Methane emissions were also reduced significantly, by up to 59.33 percent, with 12 to 18 percent devil fish.
  • The study, however, found no significant effect of the interaction of devil fish, Staphylococcus saprophyticus, and their different doses on methane production.

Conclusion

  • The research presented the possibility of using ensiled devil fish and Staphylococcus saprophyticus in horse diets as substitutes to corn.
  • These dietary additions showed promising results in reducing the emission of greenhouse gases, thereby contributing to mitigation of climate change.

Cite This Article

APA
García EDA, Khusro A, Pacheco EBF, Adegbeye MJ, Barbabosa-Pliego A, Lagunas BC, Salas JMC, Mateos RG, Aarti C, Elghandour MMMY. (2019). Influence of Dietary Supplementation of Ensiled Devil Fish and Staphylococcus saprophyticus on Equine Fecal Greenhouse Gases Production. J Equine Vet Sci, 79, 105-112. https://doi.org/10.1016/j.jevs.2019.05.023

Publication

ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 79
Pages: 105-112

Researcher Affiliations

García, E D Aguilar
  • Unidad Académica de Ciencias Agropecuarias y Ambientales, Universidad Autónoma de Guerrero, Iguala de la Independencia, Guerrero, Mexico.
Khusro, A
  • Research Department of Plant Biology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India.
Pacheco, E B Figueroa
  • Unidad Académica de Ciencias Agropecuarias y Ambientales, Universidad Autónoma de Guerrero, Iguala de la Independencia, Guerrero, Mexico.
Adegbeye, Moyosore J
  • Department of Animal Science and Livestock Production, Joseph Ayo Babalola University Ikeji-Arakeji, Ilesa, Nigeria.
Barbabosa-Pliego, A
  • Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, Mexico.
Lagunas, B Cruz
  • Unidad Académica de Ciencias Agropecuarias y Ambientales, Universidad Autónoma de Guerrero, Iguala de la Independencia, Guerrero, Mexico.
Salas, J M Castro
  • Unidad Académica de Ciencias Agropecuarias y Ambientales, Universidad Autónoma de Guerrero, Iguala de la Independencia, Guerrero, Mexico.
Mateos, R González
  • Unidad Académica de Ciencias Agropecuarias y Ambientales, Universidad Autónoma de Guerrero, Iguala de la Independencia, Guerrero, Mexico.
Aarti, C
  • Research Department of Plant Biology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India.
Elghandour, Mona M M Y
  • Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, Mexico. Electronic address: mmohamede@uaemex.mx.

MeSH Terms

  • Animals
  • Dietary Supplements / analysis
  • Greenhouse Gases
  • Horses
  • Rumen / drug effects
  • Silage / analysis
  • Staphylococcus saprophyticus