FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Biomaterials / Influence of different collagen species on physico-chemical ...
Biomaterials2004; 25(14); 2831-2841; doi: 10.1016/j.biomaterials.2003.09.066

Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices.

Abstract: Collagen-based scaffolds are appealing products for the repair of cartilage defects using tissue engineering strategies. The present study investigated the species-related differences of collagen scaffolds with and without 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)-crosslinking. Resistance against collagenase digestion, swelling ratio, amino acid sequence, shrinkage temperature, ultrastructural matrix morphology, crosslinking density and stress-strain characteristics were determined to evaluate the physico-chemical properties of equine- and bovine-collagen-based scaffolds. Three-factor ANOVA analysis revealed a highly significant effect of collagen type (p=0.0001), crosslinking (p=0.0001) and time (p=0.0001) on degradation of the collagen samples by collagenase treatment. Crosslinked equine collagen samples showed a significantly reduced swelling ratio compared to bovine collagen samples (p< 0.0001). The amino acid composition of equine collagen revealed a higher amount of hydroxylysine and lysine. Shrinkage temperatures of non-crosslinked samples showed a significant difference between equine (60 degrees C) and bovine collagen (57 degrees C). Three-factor ANOVA analysis revealed a highly significant effect of collagen type (p=0.0001), crosslinking (p=0.0001) and matrix condition (p=0.0001) on rupture strength measured by stress-strain analysis. The ultrastructure, the crosslinking density and the strain at rupture between collagen matrices of both species showed no significant differences. For tissue engineering purposes, the higher enzymatic stability, the higher form stability, as well as the lower risk of transmissible disease make the case for considering equine-based collagen. This study also indicates that results obtained for scaffolds based on a certain collagen species may not be transferable to scaffolds based on another, because of the differing physico-chemical properties.
Get Full Text
Publication Date: 2004-02-14 PubMed ID: 14962561DOI: 10.1016/j.biomaterials.2003.09.066Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Comparative Study
  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the differences between collagen scaffolds derived from equine (horse) and bovine (cow) sources. It presents findings on how these differences affect the scaffolds’ physical and chemical properties, with potential implications for their use in tissue engineering.

Methodology

  • In this study, researchers compared the properties of collagen scaffolds based on two different species: equine and bovine.
  • The collagen scaffolds were either non-crosslinked or crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) / N-hydroxysuccinimide (NHS). Crosslinking is a process that connects two different polymer chains, in this case collagen, to form a complex that can hold its shape better.
  • The researchers examined multiple properties of the scaffolds, including their resistance against collagenase digestion, swelling ratio, amino acid sequence, shrinkage temperature, matrix morphology, crosslinking density, and stress-strain characteristics.

Findings

  • The analysis showed that collagen type, crosslinking, and time significantly affected the degradation of the collagen samples when treated with collagenase, an enzyme that breaks down collagen.
  • The study found that equine collagen samples that were crosslinked displayed a significantly lower swelling ratio compared to similar bovine collagen samples.
  • The amino acid composition of equine collagen had more hydroxylysine and lysine compared to bovine collagen.
  • The shrinkage temperatures, which is the temperature at which a material starts to shrink, differed between equine (60 degrees C) and bovine collagen (57 degrees C) in non-crosslinked samples.
  • The collagen type, crosslinking, and matrix condition significantly impacted the rupture strength of the collagen matrices, as measured by stress-strain analysis.
  • There were no significant differences in the ultrastructure, or the fine details of a structure visible only with great magnification, and the crosslinking density, or the amount of crosslinks per unit volume, between the equine and bovine collagen matrices. Similarly, there were no significant differences in the strain at rupture between the two types of collagen matrices.

Implications

  • Given their higher enzymatic stability and form stability, and lower risk of transmitting disease, the researchers suggest that equine-based collagen could be more suitable for tissue engineering applications.
  • The study’s results also underline that findings for collagen scaffolds derived from one species may not necessarily apply to scaffolds from another species, due to differences in their physical and chemical properties.

Cite This Article

APA
Angele P, Abke J, Kujat R, Faltermeier H, Schumann D, Nerlich M, Kinner B, Englert C, Ruszczak Z, Mehrl R, Mueller R. (2004). Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices. Biomaterials, 25(14), 2831-2841. https://doi.org/10.1016/j.biomaterials.2003.09.066

Publication

Biomaterials
ISSN: 0142-9612
NlmUniqueID: 8100316
Country: Netherlands
Language: English
Volume: 25
Issue: 14
Pages: 2831-2841

Researcher Affiliations

Angele, Peter
  • Department of Trauma Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg 93051, Germany. angelepeter@aol.com
Abke, Jochen
    Kujat, Richard
      Faltermeier, Hubert
        Schumann, Detlef
          Nerlich, Michael
            Kinner, Bernd
              Englert, Carsten
                Ruszczak, Zbigniew
                  Mehrl, Robert
                    Mueller, Rainer

                      MeSH Terms

                      • Animals
                      • Cattle
                      • Collagen Type I / chemistry
                      • Collagen Type I / ultrastructure
                      • Collagenases / chemistry
                      • Cross-Linking Reagents / chemistry
                      • Elasticity
                      • Ethyldimethylaminopropyl Carbodiimide / chemistry
                      • Horses
                      • Protein Conformation
                      • Species Specificity
                      • Succinimides / chemistry
                      • Tensile Strength

                      Grant Funding

                      • 1 R01 AR-48132-01 / NIAMS NIH HHS

                      Citations

                      This article has been cited 67 times.
                      1. Tampieri A, Kon E, Sandri M, Campodoni E, Dapporto M, Sprio S. Marine-Inspired Approaches as a Smart Tool to Face Osteochondral Regeneration.. Mar Drugs 2023 Mar 28;21(4).
                        doi: 10.3390/md21040212pubmed: 37103351google scholar: lookup
                      2. Baltazar T, Jiang B, Moncayo A, Merola J, Albanna MZ, Saltzman WM, Pober JS. 3D bioprinting of an implantable xeno-free vascularized human skin graft.. Bioeng Transl Med 2023 Jan;8(1):e10324.
                        doi: 10.1002/btm2.10324pubmed: 36684084google scholar: lookup
                      3. Fu C, Shi S, Tian J, Gu H, Yao L, Xiao J. Non-denatured yak type I collagen accelerates sunburned skin healing by stimulating and replenishing dermal collagen.. Biotechnol Rep (Amst) 2023 Mar;37:e00778.
                        doi: 10.1016/j.btre.2022.e00778pubmed: 36578365google scholar: lookup
                      4. Ren Y, Fan L, Alkildani S, Liu L, Emmert S, Najman S, Rimashevskiy D, Schnettler R, Jung O, Xiong X, Barbeck M. Barrier Membranes for Guided Bone Regeneration (GBR): A Focus on Recent Advances in Collagen Membranes.. Int J Mol Sci 2022 Nov 29;23(23).
                        doi: 10.3390/ijms232314987pubmed: 36499315google scholar: lookup
                      5. Feng Y, Guo W, Hu L, Yi X, Tang F. Application of Hydrogels as Sustained-Release Drug Carriers in Bone Defect Repair.. Polymers (Basel) 2022 Nov 14;14(22).
                        doi: 10.3390/polym14224906pubmed: 36433033google scholar: lookup
                      6. Martins E, Diogo GS, Pires R, Reis RL, Silva TH. 3D Biocomposites Comprising Marine Collagen and Silica-Based Materials Inspired on the Composition of Marine Sponge Skeletons Envisaging Bone Tissue Regeneration.. Mar Drugs 2022 Nov 16;20(11).
                        doi: 10.3390/md20110718pubmed: 36421996google scholar: lookup
                      7. Nagaraj A, Etxeberria AE, Naffa R, Zidan G, Seyfoddin A. 3D-Printed Hybrid Collagen/GelMA Hydrogels for Tissue Engineering Applications.. Biology (Basel) 2022 Oct 25;11(11).
                        doi: 10.3390/biology11111561pubmed: 36358262google scholar: lookup
                      8. Islam MM, Chivu A, AbuSamra DB, Saha A, Chowdhuri S, Pramanik B, Dohlman CH, Das D, Argüeso P, Rajaiya J, Patra HK, Chodosh J. Crosslinker-free collagen gelation for corneal regeneration.. Sci Rep 2022 Jun 1;12(1):9108.
                        doi: 10.1038/s41598-022-13146-9pubmed: 35650270google scholar: lookup
                      9. Lindner C, Alkildani S, Stojanovic S, Najman S, Jung O, Barbeck M. In Vivo Biocompatibility Analysis of a Novel Barrier Membrane Based on Bovine Dermis-Derived Collagen for Guided Bone Regeneration (GBR).. Membranes (Basel) 2022 Mar 30;12(4).
                        doi: 10.3390/membranes12040378pubmed: 35448348google scholar: lookup
                      10. Jiang YH, Lou YY, Li TH, Liu BZ, Chen K, Zhang D, Li T. Cross-linking methods of type I collagen-based scaffolds for cartilage tissue engineering.. Am J Transl Res 2022;14(2):1146-1159.
                        pubmed: 35273719
                      11. Mbese Z, Alven S, Aderibigbe BA. Collagen-Based Nanofibers for Skin Regeneration and Wound Dressing Applications.. Polymers (Basel) 2021 Dec 13;13(24).
                        doi: 10.3390/polym13244368pubmed: 34960918google scholar: lookup
                      12. Gallo N, Natali ML, Curci C, Picerno A, Gallone A, Vulpi M, Vitarelli A, Ditonno P, Cascione M, Sallustio F, Rinaldi R, Sannino A, Salvatore L. Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration.. Materials (Basel) 2021 Dec 12;14(24).
                        doi: 10.3390/ma14247648pubmed: 34947245google scholar: lookup
                      13. Braun J, Eckes S, Kilb MF, Fischer D, Eßbach C, Rommens PM, Drees P, Schmitz K, Nickel D, Ritz U. Mechanical characterization of rose bengal and green light crosslinked collagen scaffolds for regenerative medicine.. Regen Biomater 2021 Dec;8(6):rbab059.
                        doi: 10.1093/rb/rbab059pubmed: 34858633google scholar: lookup
                      14. Haneef A, Giridhara Gopalan RO, Rajendran DT, Nunes J, Kuppamuthu D, Radhakrishnan N, Young TH, Hsieh HY, Prajna NV, Willoughby CE, Williams R. Chemical Cross-Linking of Corneal Tissue to Reduce Progression of Loss of Sight in Patients With Keratoconus.. Transl Vis Sci Technol 2021 Apr 29;10(5):6.
                        doi: 10.1167/tvst.10.5.6pubmed: 34003973google scholar: lookup
                      15. Chen X, Meng J, Xu H, Shinoda M, Kishimoto M, Sakurai S, Yamane H. Fabrication and Properties of Electrospun Collagen Tubular Scaffold Crosslinked by Physical and Chemical Treatments.. Polymers (Basel) 2021 Feb 28;13(5).
                        doi: 10.3390/polym13050755pubmed: 33670963google scholar: lookup
                      16. Sorushanova A, Skoufos I, Tzora A, Mullen AM, Zeugolis DI. The influence of animal species, gender and tissue on the structural, biophysical, biochemical and biological properties of collagen sponges.. J Mater Sci Mater Med 2021 Jan 21;32(1):12.
                        doi: 10.1007/s10856-020-06485-4pubmed: 33475864google scholar: lookup
                      17. Patil VA, Masters KS. Engineered Collagen Matrices.. Bioengineering (Basel) 2020 Dec 16;7(4).
                        doi: 10.3390/bioengineering7040163pubmed: 33339157google scholar: lookup
                      18. Gallo N, Natali ML, Sannino A, Salvatore L. An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications.. J Funct Biomater 2020 Nov 1;11(4).
                        doi: 10.3390/jfb11040079pubmed: 33139660google scholar: lookup
                      19. Ahn JJ, Kim HJ, Bae EB, Cho WT, Choi Y, Hwang SH, Jeong CM, Huh JB. Evaluation of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Cross-Linked Collagen Membranes for Guided Bone Regeneration in Beagle Dogs.. Materials (Basel) 2020 Oct 15;13(20).
                        doi: 10.3390/ma13204599pubmed: 33076566google scholar: lookup
                      20. Krok-Borkowicz M, Reczyńska K, Rumian Ł, Menaszek E, Orzelski M, Malisz P, Silmanowicz P, Dobrzyński P, Pamuła E. Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects-In Vivo Studies on Rabbits.. Int J Mol Sci 2020 Oct 13;21(20).
                        doi: 10.3390/ijms21207541pubmed: 33066080google scholar: lookup
                      21. Senadheera TRL, Dave D, Shahidi F. Sea Cucumber Derived Type I Collagen: A Comprehensive Review.. Mar Drugs 2020 Sep 18;18(9).
                        doi: 10.3390/md18090471pubmed: 32961970google scholar: lookup
                      22. Montalbano G, Borciani G, Cerqueni G, Licini C, Banche-Niclot F, Janner D, Sola S, Fiorilli S, Mattioli-Belmonte M, Ciapetti G, Vitale-Brovarone C. Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy.. Nanomaterials (Basel) 2020 Aug 27;10(9).
                        doi: 10.3390/nano10091681pubmed: 32867075google scholar: lookup
                      23. Hayashi K, Tokuda A, Nakamura J, Sugawara-Narutaki A, Ohtsuki C. Tearable and Fillable Composite Sponges Capable of Heat Generation and Drug Release in Response to Alternating Magnetic Field.. Materials (Basel) 2020 Aug 17;13(16).
                        doi: 10.3390/ma13163637pubmed: 32824485google scholar: lookup
                      24. Pasculli A, Gurrado A, De Luca GM, Mele A, Marzullo A, Mangone A, Cellamare S, Ferraro V, Maqoud F, Caggiani MC, Rana F, Cavallaro G, Prete FP, Tricarico D, Altomare CD, Testini M. Bridging repair of the abdominal wall in a rat experimental model. Comparison between uncoated and polyethylene oxide-coated equine pericardium meshes.. Sci Rep 2020 Apr 24;10(1):6959.
                        doi: 10.1038/s41598-020-63886-9pubmed: 32332926google scholar: lookup
                      25. Hong I, Khalid AW, Pae HC, Cha JK, Lee JS, Paik JW, Jung UW, Choi SH. Distinctive bone regeneration of calvarial defects using biphasic calcium phosphate supplemented ultraviolet-crosslinked collagen membrane.. J Periodontal Implant Sci 2020 Feb;50(1):14-27.
                        doi: 10.5051/jpis.2020.50.1.14pubmed: 32128270google scholar: lookup
                      26. Chen X, Zhou L, Xu H, Yamamoto M, Shinoda M, Kishimoto M, Tanaka T, Yamane H. Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film.. Materials (Basel) 2020 Jan 14;13(2).
                        doi: 10.3390/ma13020377pubmed: 31947582google scholar: lookup
                      27. Li S, Li M, Gu L, Peng L, Deng Y, Zhong J, Wang B, Wang Q, Xiao Y, Yuan J. Risk factors influencing survival of acellular porcine corneal stroma in infectious keratitis: a prospective clinical study.. J Transl Med 2019 Dec 30;17(1):434.
                        doi: 10.1186/s12967-019-02192-zpubmed: 31900186google scholar: lookup
                      28. Montalbano G, Borciani G, Pontremoli C, Ciapetti G, Mattioli-Belmonte M, Fiorilli S, Vitale-Brovarone C. Development and Biocompatibility of Collagen-Based Composites Enriched with Nanoparticles of Strontium Containing Mesoporous Glass.. Materials (Basel) 2019 Nov 11;12(22).
                        doi: 10.3390/ma12223719pubmed: 31717980google scholar: lookup
                      29. Jiang H, Zheng M, Liu X, Zhang S, Wang X, Chen Y, Hou M, Zhu J. Feasibility Study of Tissue Transglutaminase for Self-Catalytic Cross-Linking of Self-Assembled Collagen Fibril Hydrogel and Its Promising Application in Wound Healing Promotion.. ACS Omega 2019 Jul 31;4(7):12606-12615.
                        doi: 10.1021/acsomega.9b01274pubmed: 31460381google scholar: lookup
                      30. Meyer M. Processing of collagen based biomaterials and the resulting materials properties.. Biomed Eng Online 2019 Mar 18;18(1):24.
                        doi: 10.1186/s12938-019-0647-0pubmed: 30885217google scholar: lookup
                      31. Iwashita M, Ohta H, Fujisawa T, Cho M, Ikeya M, Kidoaki S, Kosodo Y. Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells.. Sci Rep 2019 Feb 28;9(1):3068.
                        doi: 10.1038/s41598-018-38395-5pubmed: 30816128google scholar: lookup
                      32. Law JX, Liau LL, Saim A, Yang Y, Idrus R. Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering.. Tissue Eng Regen Med 2017 Dec;14(6):699-718.
                        doi: 10.1007/s13770-017-0075-9pubmed: 30603521google scholar: lookup
                      33. Diogo GS, Senra EL, Pirraco RP, Canadas RF, Fernandes EM, Serra J, Pérez-Martín RI, Sotelo CG, Marques AP, González P, Moreira-Silva J, Silva TH, Reis RL. Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications.. Mar Drugs 2018 Aug 3;16(8).
                        doi: 10.3390/md16080269pubmed: 30081528google scholar: lookup
                      34. Montalbano G, Fiorilli S, Caneschi A, Vitale-Brovarone C. Type I Collagen and Strontium-Containing Mesoporous Glass Particles as Hybrid Material for 3D Printing of Bone-Like Materials.. Materials (Basel) 2018 Apr 28;11(5).
                        doi: 10.3390/ma11050700pubmed: 29710811google scholar: lookup
                      35. Rezvanian P, Daza R, López PA, Ramos M, González-Nieto D, Elices M, Guinea GV, Pérez-Rigueiro J. Enhanced Biological Response of AVS-Functionalized Ti-6Al-4V Alloy through Covalent Immobilization of Collagen.. Sci Rep 2018 Feb 20;8(1):3337.
                        doi: 10.1038/s41598-018-21685-3pubmed: 29463865google scholar: lookup
                      36. Böhm S, Strauß C, Stoiber S, Kasper C, Charwat V. Impact of Source and Manufacturing of Collagen Matrices on Fibroblast Cell Growth and Platelet Aggregation.. Materials (Basel) 2017 Sep 15;10(9).
                        doi: 10.3390/ma10091086pubmed: 28914792google scholar: lookup
                      37. Sheikh Z, Javaid MA, Hamdan N, Hashmi R. Bone Regeneration Using Bone Morphogenetic Proteins and Various Biomaterial Carriers.. Materials (Basel) 2015 Apr 15;8(4):1778-1816.
                        doi: 10.3390/ma8041778pubmed: 28788032google scholar: lookup
                      38. Ter Horst B, Chouhan G, Moiemen NS, Grover LM. Advances in keratinocyte delivery in burn wound care.. Adv Drug Deliv Rev 2018 Jan 1;123:18-32.
                        doi: 10.1016/j.addr.2017.06.012pubmed: 28668483google scholar: lookup
                      39. Wang Y, Wang X, Shang J, Liu H, Yuan Y, Guo Y, Huang B, Zhou Y. Repairing the ruptured annular fibrosus by using type I collagen combined with citric acid, EDC and NHS: an in vivo study.. Eur Spine J 2017 Mar;26(3):884-893.
                        doi: 10.1007/s00586-016-4898-1pubmed: 28004245google scholar: lookup
                      40. Ghodbane SA, Dunn MG. Physical and mechanical properties of cross-linked type I collagen scaffolds derived from bovine, porcine, and ovine tendons.. J Biomed Mater Res A 2016 Nov;104(11):2685-92.
                        doi: 10.1002/jbm.a.35813pubmed: 27325579google scholar: lookup
                      41. Babczyk P, Conzendorf C, Klose J, Schulze M, Harre K, Tobiasch E. Stem Cells on Biomaterials for Synthetic Grafts to Promote Vascular Healing.. J Clin Med 2014 Jan 15;3(1):39-87.
                        doi: 10.3390/jcm3010039pubmed: 26237251google scholar: lookup
                      42. Munoz-Pinto DJ, Jimenez-Vergara AC, Gharat TP, Hahn MS. Characterization of sequential collagen-poly(ethylene glycol) diacrylate interpenetrating networks and initial assessment of their potential for vascular tissue engineering.. Biomaterials 2015 Feb;40:32-42.
                        doi: 10.1016/j.biomaterials.2014.10.051pubmed: 25433604google scholar: lookup
                      43. Liu J, Huang Q, Liu ZB, Lin X, Zhang LH, Lin CQ, Zheng ZY. A highly sensitive solid substrate room temperature phosphorimetry for carbaryl detection based on its activating effect on NaIO4 oxidizing fluorescein.. J Fluoresc 2014 Nov;24(6):1775-83.
                        doi: 10.1007/s10895-014-1466-0pubmed: 25294182google scholar: lookup
                      44. Benedetto CD, Barbaglio A, Martinello T, Alongi V, Fassini D, Cullorà E, Patruno M, Bonasoro F, Barbosa MA, Carnevali MD, Sugni M. Production, characterization and biocompatibility of marine collagen matrices from an alternative and sustainable source: the sea urchin Paracentrotus lividus.. Mar Drugs 2014 Sep 24;12(9):4912-33.
                        doi: 10.3390/md12094912pubmed: 25255130google scholar: lookup
                      45. Koh LB, Islam MM, Mitra D, Noel CW, Merrett K, Odorcic S, Fagerholm P, Jackson WB, Liedberg B, Phopase J, Griffith M. Epoxy cross-linked collagen and collagen-laminin Peptide hydrogels as corneal substitutes.. J Funct Biomater 2013 Aug 28;4(3):162-77.
                        doi: 10.3390/jfb4030162pubmed: 24956085google scholar: lookup
                      46. König U, Lode A, Welzel PB, Ueda Y, Knaack S, Henß A, Hauswald A, Gelinsky M. Heparinization of a biomimetic bone matrix: integration of heparin during matrix synthesis versus adsorptive post surface modification.. J Mater Sci Mater Med 2014 Mar;25(3):607-21.
                        doi: 10.1007/s10856-013-5098-8pubmed: 24254473google scholar: lookup
                      47. Miguel FB, Barbosa Júnior Ade A, de Paula FL, Barreto IC, Goissis G, Rosa FP. Regeneration of critical bone defects with anionic collagen matrix as scaffolds.. J Mater Sci Mater Med 2013 Nov;24(11):2567-75.
                        doi: 10.1007/s10856-013-4980-8pubmed: 23784007google scholar: lookup
                      48. Suki B, Stamenović D, Hubmayr R. Lung parenchymal mechanics.. Compr Physiol 2011 Jul;1(3):1317-51.
                        doi: 10.1002/cphy.c100033pubmed: 23733644google scholar: lookup
                      49. Steele TW, Huang CL, Nguyen E, Sarig U, Kumar S, Widjaja E, Loo JS, Machluf M, Boey F, Vukadinovic Z, Hilfiker A, Venkatraman SS. Collagen-cellulose composite thin films that mimic soft-tissue and allow stem-cell orientation.. J Mater Sci Mater Med 2013 Aug;24(8):2013-27.
                        doi: 10.1007/s10856-013-4940-3pubmed: 23670603google scholar: lookup
                      50. Bermueller C, Schwarz S, Elsaesser AF, Sewing J, Baur N, von Bomhard A, Scheithauer M, Notbohm H, Rotter N. Marine collagen scaffolds for nasal cartilage repair: prevention of nasal septal perforations in a new orthotopic rat model using tissue engineering techniques.. Tissue Eng Part A 2013 Oct;19(19-20):2201-14.
                        doi: 10.1089/ten.TEA.2012.0650pubmed: 23621795google scholar: lookup
                      51. Bellas E, Panilaitis BJ, Glettig DL, Kirker-Head CA, Yoo JJ, Marra KG, Rubin JP, Kaplan DL. Sustained volume retention in vivo with adipocyte and lipoaspirate seeded silk scaffolds.. Biomaterials 2013 Apr;34(12):2960-8.
                        doi: 10.1016/j.biomaterials.2013.01.058pubmed: 23374707google scholar: lookup
                      52. Roessner ED, Vitacolonna M, Hohenberger P. Confocal laser scanning microscopy evaluation of an acellular dermis tissue transplant (Epiflex®).. PLoS One 2012;7(10):e45991.
                        doi: 10.1371/journal.pone.0045991pubmed: 23056225google scholar: lookup
                      53. Wodewotzky TI, Lima-Neto JF, Pereira-Júnior OC, Sudano MJ, Lima SA, Bersano PR, Yoshioka SA, Landim-Alvarenga FC. In vitro cultivation of canine multipotent mesenchymal stromal cells on collagen membranes treated with hyaluronic acid for cell therapy and tissue regeneration.. Braz J Med Biol Res 2012 Dec;45(12):1157-62.
                        doi: 10.1590/s0100-879x2012007500149pubmed: 22983182google scholar: lookup
                      54. Ng KW, Wanivenhaus F, Chen T, Abrams VD, Torzilli PA, Warren RF, Maher SA. Differential cross-linking and radio-protective effects of genipin on mature bovine and human patella tendons.. Cell Tissue Bank 2013 Mar;14(1):21-32.
                        doi: 10.1007/s10561-012-9295-3pubmed: 22350064google scholar: lookup
                      55. Tsai SW, Liou HM, Lin CJ, Kuo KL, Hung YS, Weng RC, Hsu FY. MG63 osteoblast-like cells exhibit different behavior when grown on electrospun collagen matrix versus electrospun gelatin matrix.. PLoS One 2012;7(2):e31200.
                        doi: 10.1371/journal.pone.0031200pubmed: 22319618google scholar: lookup
                      56. Liu JM, Lin LP, Liu ZB, Yang ML, Wang XX, Zhang LH, Cui ML, Jiao L. A promising CdSe@CdS-quantum dots-cysteine for the determination of trace IgE by solid substrate room temperature phosphorescence immunoassay.. J Fluoresc 2012 Jan;22(1):419-29.
                        doi: 10.1007/s10895-011-0975-3pubmed: 21947608google scholar: lookup
                      57. Schmidt T, Stachon S, Mack A, Rohde M, Just L. Evaluation of a thin and mechanically stable collagen cell carrier.. Tissue Eng Part C Methods 2011 Dec;17(12):1161-70.
                        doi: 10.1089/ten.TEC.2011.0201pubmed: 21902619google scholar: lookup
                      58. Guziewicz N, Best A, Perez-Ramirez B, Kaplan DL. Lyophilized silk fibroin hydrogels for the sustained local delivery of therapeutic monoclonal antibodies.. Biomaterials 2011 Apr;32(10):2642-50.
                        doi: 10.1016/j.biomaterials.2010.12.023pubmed: 21216004google scholar: lookup
                      59. Wu JM, Xu YY, Li ZH, Yuan XY, Wang PF, Zhang XZ, Liu YQ, Guan J, Guo Y, Li RX, Zhang H. Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor.. J Mater Sci Mater Med 2011 Jan;22(1):107-14.
                        doi: 10.1007/s10856-010-4176-4pubmed: 21052795google scholar: lookup
                      60. Walton RS, Brand DD, Czernuszka JT. Influence of telopeptides, fibrils and crosslinking on physicochemical properties of type I collagen films.. J Mater Sci Mater Med 2010 Feb;21(2):451-61.
                        doi: 10.1007/s10856-009-3910-2pubmed: 19851839google scholar: lookup
                      61. Schoen I, Rahne T, Markwart A, Neumann K, Berghaus A, Roepke E. Cartilage replacement by use of hybrid systems of autologous cells and polyethylene: an experimental study.. J Mater Sci Mater Med 2009 Oct;20(10):2145-54.
                        doi: 10.1007/s10856-009-3775-4pubmed: 19455408google scholar: lookup
                      62. Angele P, Müller R, Schumann D, Englert C, Zellner J, Johnstone B, Yoo J, Hammer J, Fierlbeck J, Angele MK, Nerlich M, Kujat R. Characterization of esterified hyaluronan-gelatin polymer composites suitable for chondrogenic differentiation of mesenchymal stem cells.. J Biomed Mater Res A 2009 Nov;91(2):416-27.
                        doi: 10.1002/jbm.a.32236pubmed: 18985778google scholar: lookup
                      63. Suki B, Bates JH. Extracellular matrix mechanics in lung parenchymal diseases.. Respir Physiol Neurobiol 2008 Nov 30;163(1-3):33-43.
                        doi: 10.1016/j.resp.2008.03.015pubmed: 18485836google scholar: lookup
                      64. Al-Munajjed AA, Hien M, Kujat R, Gleeson JP, Hammer J. Influence of pore size on tensile strength, permeability and porosity of hyaluronan-collagen scaffolds.. J Mater Sci Mater Med 2008 Aug;19(8):2859-64.
                        doi: 10.1007/s10856-008-3422-5pubmed: 18347950google scholar: lookup
                      65. Cao H, Xu SY. EDC/NHS-crosslinked type II collagen-chondroitin sulfate scaffold: characterization and in vitro evaluation.. J Mater Sci Mater Med 2008 Feb;19(2):567-75.
                        doi: 10.1007/s10856-007-3281-5pubmed: 18058201google scholar: lookup
                      66. Englert C, Blunk T, Müller R, von Glasser SS, Baumer J, Fierlbeck J, Heid IM, Nerlich M, Hammer J. Bonding of articular cartilage using a combination of biochemical degradation and surface cross-linking.. Arthritis Res Ther 2007;9(3):R47.
                        doi: 10.1186/ar2202pubmed: 17504533google scholar: lookup
                      67. Wahl DA, Sachlos E, Liu C, Czernuszka JT. Controlling the processing of collagen-hydroxyapatite scaffolds for bone tissue engineering.. J Mater Sci Mater Med 2007 Feb;18(2):201-9.
                        doi: 10.1007/s10856-006-0682-9pubmed: 17323151google scholar: lookup
                      Subscribe to our newsletter
                      Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.