Influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol, and fetal calf serum on in vitro maturation and parthenogenic development in equine oocytes.
Abstract: The effects of insulin-like growth factor-I (IGF-I) and its interaction with gonadotropins, estradiol, and fetal calf serum (FCS) on in vitro maturation (IVM) of equine oocytes were investigated in this study. We also examined the role of IGF-I in the presence or absence of gonadotropins, estradiol, and FCS in parthenogenic cleavage after oocyte activation with calcium ionophore combined with 6-dimethylaminopurine (6-DMAP), using cleavage rate as a measure of cytoplasmic maturation. Only equine cumulus-oocyte complexes with compact cumulus and homogenous ooplasm (n = 817) were used. In experiment 1, oocytes were cultured in TCM-199 supplemented with BSA, antibiotics, and IGF-I at 0 (control), 50, 100, 200 ng/ml, at 39 degrees C in air with 5% CO(2), 95% humidity for 36 or 48 h. In experiment 2, oocytes were cultured with FSH, LH, estradiol, and FCS with IGF-I at the concentration that promoted the highest nuclear maturation rate in experiment 1. In experiment 3, oocytes from the three experimental groups (IGF-I; hormones; and IGF-I + hormones) were chemically activated by exposure to calcium ionophore followed by culture in 6-DMAP. In experiment 1, IGF-I stimulated equine oocyte maturation in a dose-dependent manner with the highest nuclear maturation rate at a concentration of 200 ng/ml. No significant effect of IGF-I on nuclear maturation was observed in experiment 2. In experiment 3, a significant difference in cleavage rate was observed between the hormone + IGF-I group (15 of 33; 45.4%) compared with IGF-I (10 of 36; 27.8%) and hormone (4 of 31; 12.9%) alone (P < 0.05). These results demonstrated that IGF-I has a positive effect on nuclear maturation rate of equine oocytes in vitro. The addition of IGF-I to an IVM medium containing hormones and FCS did not increase nuclear maturation, but resulted in a positive effect on cytoplasmic maturation of equine oocytes measured by parthenogenic cleavage.
Publication Date: 2001-08-22 PubMed ID: 11514356DOI: 10.1095/biolreprod65.3.899Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates how the insulin-like growth factor-I (IGF-I) interacts with hormones and other components while contributing to the in vitro maturation and parthenogenic development in horse oocytes.
Research Methodology
The research consisted of three experiments, each representing a unique condition that the equine cumulus-oocyte complexes were subjected to:
- In the first experiment, the oocytes were cultured under controlled conditions with doses of IGF-I which varied between 0 (control), 50, 100, 200 ng/ml. These oocytes were accumulated only if they had compact cumulus and homogenous ooplasm.
- The second experiment supplemented these oocytes with follicle-stimulating hormone (FSH), luteinising hormone (LH), estradiol, and fetal calf serum (FCS), along with IGF-I concentration that evoked the highest nuclear maturation in the first experiment.
- In the third experiment, the oocytes underwent chemical activation, with exposure to calcium ionophore followed by 6-dimethylaminopurine (6-DMAP) culture, and were categorized into three experimental groups based on presence of IGF-I and hormones.
Results And Observations
After conducting the experiments, the results and observations were as follows:
- In the first experiment, the researchers noted that IGF-I stimulated equine oocyte maturation in a dose-dependent manner, with the highest nuclear maturation seen at an IGF-I concentration of 200 ng/ml.
- In the second experiment, the researchers did not observe any significant effect of IGF-I on nuclear maturation.
- In the third experiment, the researchers observed a significant difference in cleavage rates (scores used to measure cytoplasmic maturation) between the hormone + IGF-I group, hormone alone, and IGF-I alone. The results showed that the hormone + IGF-I group achieved better cleavage rates, indicating that the presence of both hormones and IGF-I was beneficial to cytoplasmic maturation.
Conclusion
In conclusion, the study showcased that IGF-I positively influences the nuclear maturation rate of equine oocytes in vitro. Although the addition of IGF-I did not increase nuclear maturation in hormonal and FCS supplemented medium, it did have a positive effect on cytoplasmic maturation of equine oocytes. This insight could be crucial for better comprehension of oocyte maturation and potentially improving in vitro maturation procedures.
Cite This Article
APA
Carneiro G, Lorenzo P, Pimentel C, Pegoraro L, Bertolini M, Ball B, Anderson G, Liu I.
(2001).
Influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol, and fetal calf serum on in vitro maturation and parthenogenic development in equine oocytes.
Biol Reprod, 65(3), 899-905.
https://doi.org/10.1095/biolreprod65.3.899 Publication
Researcher Affiliations
- Department of Population Health & Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. gfcarneiro@ucdavis.edu
MeSH Terms
- Animals
- Cattle
- Cell Nucleus / physiology
- Cells, Cultured
- Culture Media
- Cytoplasm / physiology
- Drug Interactions
- Estradiol / pharmacology
- Female
- Fetal Blood
- Gonadotropins, Pituitary / pharmacology
- Horses
- Insulin-Like Growth Factor I / pharmacology
- Oocytes / drug effects
- Oocytes / physiology
- Oocytes / ultrastructure
- Parthenogenesis
Citations
This article has been cited 6 times.- Hisey EA, Ross PJ, Meyers S. Genetic Manipulation of the Equine Oocyte and Embryo. J Equine Vet Sci 2021 Apr;99:103394.
- Maksura H, Akon N, Islam MN, Akter I, Modak AK, Khatun A, Alam MH, Hashem MA, Amin MR, Moniruzzaman M. Effects of estradiol on in vitro maturation of buffalo and goat oocytes. Reprod Med Biol 2021 Jan;20(1):62-70.
- Liu X, Lang Q, Wu M, You X, He Q, Luo L, Liu Z, Xiao P, Huang N, Yang X, Ge L. Screening high-quality fetal bovine serum for porcine oocyte maturation in vitro. Animal Model Exp Med 2019 Dec;2(4):334-339.
- Abdel-Ghani MA, Abe Y, Asano T, Hamano S, Suzuki H. Effect of bovine cumulus-oocyte complexes-conditioned medium on in-vitro maturation of canine oocytes. Reprod Med Biol 2011 Mar;10(1):43-49.
- Castilla-Cortazar I, Diez N, Garcia-Fernandez M, Puche JE, Diez-Caballero F, Quiroga J, Diaz-Sanchez M, Castilla A, Casares AD, Varela-Nieto I, Prieto J, Gonzalez-Baron S. Hematotesticular barrier is altered from early stages of liver cirrhosis: effect of insulin-like growth factor 1. World J Gastroenterol 2004 Sep 1;10(17):2529-34.
- Dell'Aquila ME, Caillaud M, Maritato F, Martoriati A, Gérard N, Aiudi G, Minoia P, Goudet G. Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis. Reprod Biol Endocrinol 2004 Jun 22;2:44.
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