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Home / Equine Research Bank / Anim Reprod Sci / Influence of seminal plasma on fresh and post-thaw parameter...
Animal reproduction science2010; 123(3-4); 192-201; doi: 10.1016/j.anireprosci.2010.11.017

Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa.

Abstract: Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa. Six sperm categories of each stallion (n=4) were evaluated for motility, morphology and viability. These categories were fresh ejaculated spermatozoa (Fr-E), fresh epididymal spermatozoa that had been exposed to seminal plasma (Fr-SP+), fresh epididymal spermatozoa that had never been exposed to seminal plasma (Fr-SP-), frozen-thawed ejaculated spermatozoa (Cr-E), frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma prior to freezing (Cr-SP+) and frozen-thawed epididymal spermatozoa that had never been exposed to seminal plasma (Cr-SP-). Results show that seminal plasma stimulates initial motility of fresh epididymal stallion spermatozoa while this difference in progressive motility is no longer present post-thaw; and that progressive motility of fresh or frozen-thawed ejaculated stallion spermatozoa is not always a good indicator for post-thaw progressive motility of epididymal spermatozoa. This study shows that seminal plasma has a positive influence on the incidence of overall sperm defects, midpiece reflexes and distal cytoplasmic droplets in frozen-thawed stallion epididymal spermatozoa while the occurance of midpiece reflexes is likely to be linked to distal cytoplasmic droplets. Furthermore, seminal plasma does not have an influence on viability of fresh and frozen-thawed morphologically normal epididymal spermatozoa. We recommend the retrograde flushing technique using seminal plasma as flushing medium to harvest and freeze stallion epididymal spermatozoa.
Copyright © 2010 Elsevier B.V. All rights reserved.
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Publication Date: 2010-12-21 PubMed ID: 21256684DOI: 10.1016/j.anireprosci.2010.11.017Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the impact of seminal plasma on the motility, morphology, and viability of fresh and frozen-thawed stallion epididymal spermatozoa, comparing these parameters with the same features in ejaculated spermatozoa.

Understanding the Research Study

This study examines the effects of seminal plasma exposure on stallion epididymal spermatozoa, specifically evaluating fresh and post-thaw qualities such as movement, physical appearance, and longevity. To make a comprehensive assessment, six types of sperm from each of the feed stallions were examined:

  • Fresh ejaculated spermatozoa (Fr-E)
  • Fresh epididymal spermatozoa exposed to seminal plasma (Fr-SP+)
  • Fresh epididymal spermatozoa not exposed to seminal plasma (Fr-SP-)
  • Frozen-thawed ejaculated spermatozoa (Cr-E)
  • Frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma prior to freezing (Cr-SP+)
  • Frozen-thawed epididymal spermatozoa that had never been exposed to seminal plasma (Cr-SP-)

Findings of the Study

The outcomes indicate that the presence of seminal plasma augments the initial movement of fresh stallion epididymal spermatozoa, though this discrepancy in progressive motility vanishes post-thaw. It further suggests that analyzing the motility of fresh or frozen-thawed ejaculated spermatozoa is not a reliable predictor for the progressive motility of post-thaw epididymal spermatozoa.

The research also illustrates that seminal plasma positively affects the occurrence of overall sperm defects, midpiece reflexes, and distal cytoplasmic droplets in frozen-thawed stallion epididymal spermatozoa. Interestingly, the instance of midpiece reflexes appears to be directly connected to distal cytoplasmic droplets.

Despite these findings, the presence of seminal plasma emerges as having no considerable influence on the viability of both fresh and frozen-thawed morphologically normal epididymal spermatozoa.

Recommendations

The researchers recommend the use of additional techniques such as the ‘retrograde flushing technique’ with seminal plasma as the flushing medium for obtaining and freezing stallion epididymal spermatozoa, asserting the positive impact seminal plasma has on sperm attributes. These findings provide crucial insights that can inform better practices for harvesting and storage of stallion spermatozoa in breeding applications.

Cite This Article

APA
Heise A, Thompson PN, Gerber D. (2010). Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa. Anim Reprod Sci, 123(3-4), 192-201. https://doi.org/10.1016/j.anireprosci.2010.11.017

Publication

Animal reproduction science
ISSN: 1873-2232
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 123
Issue: 3-4
Pages: 192-201

Researcher Affiliations

Heise, A
  • Section of Reproduction, Department of Production Animal Studies, Faculty of Veterinary Science, 0110 Onderstepoort, South Africa. annett.heise@gmx.de
Thompson, P N
    Gerber, D

      MeSH Terms

      • Animals
      • Cell Shape / drug effects
      • Cell Survival / drug effects
      • Cell Survival / physiology
      • Cryopreservation / veterinary
      • Cryoprotective Agents / pharmacology
      • Freezing
      • Horses / physiology
      • Male
      • Semen / physiology
      • Semen Analysis
      • Semen Preservation / methods
      • Semen Preservation / veterinary
      • Sperm Count / veterinary
      • Sperm Retrieval / veterinary
      • Spermatozoa / cytology
      • Spermatozoa / drug effects
      • Spermatozoa / physiology

      Citations

      This article has been cited 6 times.
      1. Monaco D, Lacalandra GM, Ansar Z, Trerotoli P, Mulligan BP, Osman TK. The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa. Animals (Basel) 2023 Aug 22;13(17).
        doi: 10.3390/ani13172685pubmed: 37684949google scholar: lookup
      2. Podico G, Canisso IF. Retrograde Flushing Followed by Slicing Float-Up as an Approach to Optimize Epididymal Sperm Recovery for the Purpose of Cryopreservation in Equids. Animals (Basel) 2022 Jul 14;12(14).
        doi: 10.3390/ani12141802pubmed: 35883349google scholar: lookup
      3. Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation. Int J Mol Sci 2020 Sep 3;21(17).
        doi: 10.3390/ijms21176415pubmed: 32899253google scholar: lookup
      4. Loureiro KC, Lima-Verde IB, Johannisson A, Ntallaris T, Jager A, Štěpánek P, da Costa Mendonça M, Severino P, Morrell JM. Effects of cashew gum and nanoparticles on cooled stallion semen. Acta Vet Scand 2020 Jun 18;62(1):31.
        doi: 10.1186/s13028-020-00530-6pubmed: 32552825google scholar: lookup
      5. Scholkamy TH, El-Badry DA, Mahmoud KG. Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa. Iran J Vet Res 2016 Fall;17(4):253-258.
        pubmed: 28224009
      6. Thema MA, Mkhize NR, Sebopela MD, Ledwaba MR, Mphaphathi ML. Effect of Pre-Freezing 18 °C Holding Time on Post-Thaw Motility and Morphometry of Cryopreserved Boar Epididymal Sperm. Animals (Basel) 2025 Jun 7;15(12).
        doi: 10.3390/ani15121691pubmed: 40564243google scholar: lookup
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