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Home / Equine Research Bank / BMC Musculoskelet Disord / Influence of species and anatomical location on chondrocyte ...
BMC musculoskeletal disorders2005; 6; 23; doi: 10.1186/1471-2474-6-23

Influence of species and anatomical location on chondrocyte expansion.

Abstract: Bovine articular cartilage is often used to study chondrocytes in vitro. It is difficult to correlate in vitro studies using bovine chondrocytes with in vivo studies using other species such as rabbits and sheep. The aim of this investigation was to study the effect of species, anatomical location and exogenous growth factors on chondrocyte proliferation in vitro. Methods: Equine (EQ), bovine (BO) and ovine (OV) articular chondrocytes from metacarpophalangeal (fetlock (F)), shoulder (S) and knee (K) joints were cultured in tissue culture flasks. Growth factors (rh-FGFb: 10 ng/ml; rh-TGFbeta: 5 ng/ml) were added to the cultures at days 2 and 4. On day 6, cells were counted and flow cytometry analysis was performed to determine cell size and granularity. A three factor ANOVA with paired Tukey's correction was used for statistical analysis. Results: After 6 days in culture, cell numbers had increased in control groups of EQ-F, OV-S, OV-F and BO-F chondrocytes. The addition of rh-FGFb led to the highest increase in cell numbers in the BO-F, followed by EQ-F and OV-S chondrocytes. The addition of rh-TGFbeta increased cell numbers in EQ-S and EQ-F chondrocytes, but showed nearly no effect on EQ-K, OV-K, OV-S, OV-F and BO-F chondrocytes. There was an overall difference with the addition of growth factors between the different species and joints. Conclusions: Different proliferation profiles of chondrocytes from the various joints were found. Therefore, we recommend performing in vitro studies using the species and site where subsequent in vivo studies are planned.
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Publication Date: 2005-05-17 PubMed ID: 15904515PubMed Central: PMC1166560DOI: 10.1186/1471-2474-6-23Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigated the impact of species, anatomical location and added growth factors on the proliferation and growth of chondrocytes (cartilage cells). The research showed varying growth profiles for chondrocytes from different joints and species, and recommended performing experiments in species and sites where subsequent in-depth studies are planned.

Research Methodology

  • The research employed equine (EQ), bovine (BO), and ovine (OV) articular chondrocytes from the metacarpophalangeal (fetlock (F)), shoulder (S), and knee (K) joints.
  • These cells were cultured in tissue culture flasks, and certain growth factors (rh-FGFb: 10 ng/ml; rh-TGFbeta: 5 ng/ml) were added to the cultures at the second and fourth days.
  • Cell count was done on the sixth day, and flow cytometry analysis was conducted to ascertain cell size and granularity.
  • For statistical analysis, the team utilized a three-factor ANOVA with a paired Tukey’s correction.

Research Findings

  • After six days in culture, an increase in cell numbers was noticed in control groups of EQ-F, OV-S, OV-F, and BO-F chondrocytes.
  • The addition of rh-FGFb caused the highest increase in cell numbers in the BO-F, followed by EQ-F and OV-S chondrocytes.
  • rh-TGFbeta increased cell numbers in EQ-S and EQ-F chondrocytes, but exhibited nearly no effect on EQ-K, OV-K, OV-S, OV-F, and BO-F chondrocytes.
  • A noticeable difference with the addition of growth factors between the different species and joints was observed.

Conclusion and Recommendations

  • The study concluded with different proliferation profiles of chondrocytes from the various joints.
  • As such, it recommends performing in vitro studies using the species and site where subsequent in vivo studies are planned to maintain consistency and evaluate more accurately the cartilage cells’ behavior in a similar environment.

Cite This Article

APA
Akens MK, Hurtig MB. (2005). Influence of species and anatomical location on chondrocyte expansion. BMC Musculoskelet Disord, 6, 23. https://doi.org/10.1186/1471-2474-6-23

Publication

BMC musculoskeletal disorders
ISSN: 1471-2474
NlmUniqueID: 100968565
Country: England
Language: English
Volume: 6
Pages: 23

Researcher Affiliations

Akens, Margarete K
  • Dept. of Clinical Studies, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. makens@uhnres.utoronto.ca
Hurtig, Mark B

    MeSH Terms

    • Animals
    • Cartilage, Articular / cytology
    • Cattle
    • Cell Count
    • Cell Division / drug effects
    • Cell Proliferation / drug effects
    • Cell Size
    • Cells, Cultured
    • Chondrocytes / cytology
    • Chondrocytes / ultrastructure
    • Cytoplasmic Granules / ultrastructure
    • Fibroblast Growth Factor 2 / pharmacology
    • Horses
    • Humans
    • Knee Joint
    • Metacarpophalangeal Joint
    • Recombinant Proteins / pharmacology
    • Sheep
    • Shoulder Joint
    • Species Specificity
    • Transforming Growth Factor beta / pharmacology

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