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Home / Equine Research Bank / Res Vet Sci / Influence of transportation on serum concentrations of acute...
Research in veterinary science2012; 93(2); 914-917; doi: 10.1016/j.rvsc.2012.01.004

Influence of transportation on serum concentrations of acute phase proteins in horse.

Abstract: The modifications of Haptoglobin (Hp), Serum Amyloid A (SAA), Fibrinogen (Fbg) and White Blood Cells (WBCs) were evaluated in 15 Saddle Italian horses. Ten horses were transported covering a distance of about 320 km within 4 h with an average speed of 80 km/h (experimental group) and five horses were not subject to transportation (control group). Blood was collected via jugular venipuncture before the transportation (T0), immediately after the transportation (T1), 12 (T12), 24 (T24) and 48 (T48)hours after the transportation in experimental group and at the same time point in control group. For each parameter statistical analysis of different groups and sampling time was performed using a two-way analysis of covariance, with the data before the transportation (T0) as the covariate, by the GLM procedure of SAS. For all parameters the interaction (Group × Time) was tested and it was resulted no significant. The application of statistical analysis showed significant differences between the control group and horses subjected to transportation (P<0.01), and the influence of sampling time (P<0.05) on Hp, SAA and WBCs. These modifications appeared to be innovative showing that equine Hp, generally considered as moderate acute phase protein, increases more rapidly than the SAA after transportation-induced stress.
Copyright © 2012 Elsevier Ltd. All rights reserved.
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Publication Date: 2012-01-31 PubMed ID: 22296939DOI: 10.1016/j.rvsc.2012.01.004Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the effects of long-distance transportation on acute phase protein concentrations in horses. The researchers found significant changes in the levels of certain proteins and white blood cells in horses after they were transported.

Study Design and Methodology

  • The experiment involved 15 Saddle Italian horses. 10 of these horses were subjected to a 320 km journey over 4 hours, which was at an average speed of 80 km/h. This group of horses is referred to as the experimental group.
  • In contrast, five horses, known as the control group, were not exposed to transportation.
  • Blood samples were taken from all horses via jugular venipuncture at various time points – before transportation (T0), immediately after transportation (T1), 12 hours after (T12), 24 hours after (T24), and 48 hours after (T48) transportation.
  • The control group underwent the same blood draw schedule but without the transportation event.
  • The researchers then assessed changes in the levels of Haptoglobin (Hp), Serum Amyloid A (SAA), Fibrinogen (Fbg), and White Blood Cells (WBCs).

Statistical Analysis and Findings

  • A two-way analysis of covariance was applied to the gathered data, using the pre-transportation results as the covariate. This process was carried out using the General Linear Model (GLM) procedure of the Statistical Analysis System (SAS).
  • The team also examined the interaction effect between the group and time, but found it to be non-significant.
  • The findings revealed significant differences in Hp, SAA, and WBC levels between the control group and the transported horses.
  • Moreover, the time of sample collection was found to significantly influence these parameters.
  • Interestingly, Hp, which is typically considered a moderate acute phase protein, seemed to increase more quickly than SAA after transportation-induced stress of the horses.

Implications of the Study

  • The results reflect that the stress induced by transportation can significantly affect the immune response of horses, as evidenced by alterations in the levels of specific proteins and white blood cells.
  • This study provides new insights specific to equine health, suggesting that transportation stress triggers a more rapid increase in the levels of Hp compared to SAA.
  • The findings could help inform better strategies to manage and reduce stress in horses during transportation.

Cite This Article

APA
Casella S, Fazio F, Giannetto C, Giudice E, Piccione G. (2012). Influence of transportation on serum concentrations of acute phase proteins in horse. Res Vet Sci, 93(2), 914-917. https://doi.org/10.1016/j.rvsc.2012.01.004

Publication

Research in veterinary science
ISSN: 1532-2661
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 93
Issue: 2
Pages: 914-917

Researcher Affiliations

Casella, S
  • Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Italy.
Fazio, F
    Giannetto, C
      Giudice, E
        Piccione, G

          MeSH Terms

          • Acute-Phase Proteins / metabolism
          • Animals
          • Fibrinogen / metabolism
          • Haptoglobins / metabolism
          • Horses / physiology
          • Leukocyte Count
          • Serum Amyloid A Protein / metabolism
          • Stress, Physiological / physiology
          • Time Factors
          • Transportation

          Citations

          This article has been cited 17 times.
          1. Arfuso F, Rizzo M, Giannetto C, Giudice E, Piccione G, Fazio F, Cirincione R, Cassata G, Cicero L. Inflammatory-like status and acute stress response in horses after road transport. Sci Rep 2023 Jun 17;13(1):9858.
            doi: 10.1038/s41598-023-37069-1pubmed: 37330619google scholar: lookup
          2. Peixoto Rabelo I, Barroco de Paula V, Carvalho Bustamante C, Santana AM, Gomes da Silva D, Baldassi AC, Canola PA, Araújo Valadão CA. Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization. Front Vet Sci 2023;10:1043656.
            doi: 10.3389/fvets.2023.1043656pubmed: 36816195google scholar: lookup
          3. Bazzano M, Arfuso F, Bonfili L, Eleuteri AM, McLean A, Serri E, Spaterna A, Laus F. Measuring Biochemical Variables and Serum Amyloid A (SAA) in Working Mules in Central Italy. Animals (Basel) 2022 Oct 16;12(20).
            doi: 10.3390/ani12202793pubmed: 36290179google scholar: lookup
          4. Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar Schmidt C, Michel V, Miranda Chueca MÁ, Padalino B, Pasquali P, Roberts HC, Spoolder H, Stahl K, Velarde A, Viltrop A, Winckler C, Earley B, Edwards S, Faucitano L, Marti S, Miranda de La Lama GC, Costa LN, Thomsen PT, Ashe S, Mur L, Van der Stede Y, Herskin M. Welfare of equidae during transport. EFSA J 2022 Sep;20(9):e07444.
            doi: 10.2903/j.efsa.2022.7444pubmed: 36092762google scholar: lookup
          5. Przewoźny M, Senderska-Płonowska M, Rząsa A, Wierzbicki H, Borkowski J, Swagemakers JH, Żak-Bochenek A, Stefaniak T. Usefulness of Selected Acute-Phase Proteins in the Postsurgical Monitoring of Arthroscopy and Splint Bone Removal in Horses. Animals (Basel) 2021 Oct 13;11(10).
            doi: 10.3390/ani11102952pubmed: 34679973google scholar: lookup
          6. Mohamed RH, Abo El-Maaty AM, Abd El Hameed AR, Ali AH. Impact of travel by walk and road on testicular hormones, oxidants, traces minerals, and acute phase response biomarkers of dromedary camels. Heliyon 2021 Apr;7(4):e06879.
            doi: 10.1016/j.heliyon.2021.e06879pubmed: 34007918google scholar: lookup
          7. Minamijima Y, Niwa H, Uchida E, Yamamoto K. Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics. J Equine Sci 2021 Mar;32(1):11-15.
            doi: 10.1294/jes.32.11pubmed: 33776535google scholar: lookup
          8. Long A, Nolen-Walston R. Equine Inflammatory Markers in the Twenty-First Century: A Focus on Serum Amyloid A. Vet Clin North Am Equine Pract 2020 Apr;36(1):147-160.
            doi: 10.1016/j.cveq.2019.12.005pubmed: 32007299google scholar: lookup
          9. Assunção P, Barbosa T, Yonezawa L, Barbosa L, Watanabe M, Kohayagawa A, Schmidt E. Acute-phase protein profile in horses subjected to different exercise protocols. Can J Vet Res 2019 Oct;83(4):272-278.
            pubmed: 31571727
          10. Witkowska-Piłaszewicz OD, Żmigrodzka M, Winnicka A, Miśkiewicz A, Strzelec K, Cywińska A. Serum amyloid A in equine health and disease. Equine Vet J 2019 May;51(3):293-298.
            doi: 10.1111/evj.13062pubmed: 30565319google scholar: lookup
          11. Andreassen SM, Vinther AML, Nielsen SS, Andersen PH, Tnibar A, Kristensen AT, Jacobsen S. Changes in concentrations of haemostatic and inflammatory biomarkers in synovial fluid after intra-articular injection of lipopolysaccharide in horses. BMC Vet Res 2017 Jun 19;13(1):182.
            doi: 10.1186/s12917-017-1089-1pubmed: 28629364google scholar: lookup
          12. Barrachina L, Remacha AR, Romero A, Vázquez FJ, Albareda J, Prades M, Ranera B, Zaragoza P, Martín-Burriel I, Rodellar C. Inflammation affects the viability and plasticity of equine mesenchymal stem cells: possible implications in intra-articular treatments. J Vet Sci 2017 Mar 30;18(1):39-49.
            doi: 10.4142/jvs.2017.18.1.39pubmed: 27297420google scholar: lookup
          13. Bullone M, de Lagarde M, Vargas A, Lavoie JP. Serum Surfactant Protein D and Haptoglobin as Potential Biomarkers for Inflammatory Airway Disease in Horses. J Vet Intern Med 2015 Nov-Dec;29(6):1707-11.
            doi: 10.1111/jvim.13602pubmed: 26289543google scholar: lookup
          14. Fazio F, Casella S, Giannetto C, Giudice E, Piccione G. Characterization of acute phase proteins and oxidative stress response to road transportation in the dog. Exp Anim 2015;64(1):19-24.
            doi: 10.1538/expanim.14-0032pubmed: 25100022google scholar: lookup
          15. Hooijberg EH, van den Hoven R, Tichy A, Schwendenwein I. Diagnostic and predictive capability of routine laboratory tests for the diagnosis and staging of equine inflammatory disease. J Vet Intern Med 2014 Sep-Oct;28(5):1587-93.
            doi: 10.1111/jvim.12404pubmed: 25056342google scholar: lookup
          16. Cywinska A, Witkowski L, Szarska E, Schollenberger A, Winnicka A. Serum amyloid A (SAA) concentration after training sessions in Arabian race and endurance horses. BMC Vet Res 2013 May 1;9:91.
            doi: 10.1186/1746-6148-9-91pubmed: 23634727google scholar: lookup
          17. Yaghoobpour T, Sheikhi Z, Nazifi S. The impact of stress in domestic animals: roles of heat shock proteins and acute-phase proteins. Vet Res Commun 2025 Jul 17;49(5):258.
            doi: 10.1007/s11259-025-10802-zpubmed: 40673978google scholar: lookup
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