Innervation of the equine mature and immature proximal sesamoid bone by calcitonin gene-related peptide and substance P-containing nerves.
Abstract: To localize and determine relative frequency of occurrence of calcitonin gene-related peptide (CGRP) and substance P (SP) fibers in the proximal sesamoid bones (PSB) and adjacent structures in sound horses. Methods: 4 foals and 3 adult horses. Methods: Medial PSB and adjacent ligaments of both forelimbs were collected, flushed, and fixed in buffered 4% formalin. The left PSB were cut into 5 longitudinal, sagittally oriented slabs, and the right PSB were cut into 5 transverse slabs. After decalcification in EDTA, slices were transferred to a 30% sucrose solution, deep frozen, sectioned (80 microm), and stored in Tris-buffered saline solution for free-floating immunocytochemical processing, staining of which was performed by use of the peroxidase-antiperoxidase method with polyclonal rabbit antibodies against CGRP and SP. Sections were examined by light microscopy. Results: Calcitonin gene-related peptide and SP fibers were present throughout the PSB; in ligaments, they were concentrated at the attachment sites. Presentation of the fibers, the innervation pattern, and intensity in the bones of horses differed from that in foals, in which the fibers were more delicate and frequency of occurrence was lower. In horses, innervation with CGRP and SP fibers was more dense in the periphery of the bone, whereas in foals, local difference in innervation of the bone was not evident. With the exception of the intersesamoidean ligament, ligaments were more richly innervated than was bone. Conclusions: Presence of the nociceptive peptides CGRP and SP indicated that the PSB and, more practically, the adjacent ligaments are sensitive structures.
Publication Date: 1998-11-26 PubMed ID: 9829393
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- Journal Article
Summary
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This research study examines the location and prevalence of calcitonin gene-related peptide (CGRP) and substance P fibers in the proximal sesamoid bones (PSB) and nearby structures in healthy horses. The study reveals variations in nerve fiber presentation, nerve patterns, and intensity among adult horses and foals.
Methods
- The study was conducted on 4 foals and 3 adult horses. Their medial PSB and adjacent ligaments from both forelimbs were collected, flushed, and fixed in a buffered 4% formalin solution.
- The left PSB were cut into 5 longitudinal, sagittally oriented slabs while the right PSB was cut into 5 transverse slabs.
- Post decalcification, the slices were subjected to a 30% sucrose solution and deep-frozen. They were then sectioned (80 microm) and stored in a Tris-buffered saline solution for free-floating immunocytochemical processing.
- Subsequently, staining was performed using the peroxidase-antiperoxidase method with polyclonal rabbit antibodies against CGRP and SP.
- Lastly, the researchers examined the sections by light microscopy.
Results
- It was found that CGRP and SP fibers were present throughout the PSB of the healthy horses. Within ligaments, these fibers were more concentrated at the attachment sites.
- Notable differences were observed in the presentation of fibers, the innervation pattern, and intensity between adult horses and foals. Fibers in foals were more delicate and less frequently present.
- Denser innervation with CGRP and SP fibers was found in the periphery of the bone in adult horses, while such local difference in innervation was not evident in foals.
- Ligaments were more richly innervated than the bone, with the exception of the intersesamoidean ligament.
Conclusions
- The research concluded that the presence of these nociceptive peptides (Pain related) – CGRP and SP – indicates that both the PSB and the adjacent ligaments are sensitive structures.
Cite This Article
APA
Cornelissen BP, Buma P, Rijkenhuizen AB, Barneveld A.
(1998).
Innervation of the equine mature and immature proximal sesamoid bone by calcitonin gene-related peptide and substance P-containing nerves.
Am J Vet Res, 59(11), 1378-1385.
Publication
Researcher Affiliations
- Department of General and Large Animal Surgery, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
MeSH Terms
- Aging
- Animals
- Calcitonin Gene-Related Peptide / analysis
- Female
- Forelimb / innervation
- Horses / anatomy & histology
- Immunoenzyme Techniques / veterinary
- Male
- Nerve Fibers / chemistry
- Rabbits
- Sesamoid Bones / innervation
- Substance P / analysis
- Sympathetic Nervous System / chemistry
- Tyrosine 3-Monooxygenase / analysis
Citations
This article has been cited 1 times.- Jimenez-Andrade JM, Mantyh WG, Bloom AP, Freeman KT, Ghilardi JR, Kuskowski MA, Mantyh PW. The effect of aging on the density of the sensory nerve fiber innervation of bone and acute skeletal pain. Neurobiol Aging 2012 May;33(5):921-32.
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