FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Acta Vet Scand / Insemination results with slow-cooled stallion semen stored ...
Acta veterinaria Scandinavica1994; 35(3); 257-262; doi: 10.1186/BF03548330

Insemination results with slow-cooled stallion semen stored for approximately 40 hours.

Abstract: Semen from 3 stallions was extended using 2 methods (Kenney extender and a modified Kenney extender), slowly cooled, and stored for 41 +/- 6 (s.d.) h before insemination. An insemination dose (40 ml) contained 1.5-2 billion spermatozoa. In the experiment, 26 mares were inseminated in 30 cycles. The pregnancy rate per cycle obtained with sperm stored in the Kenney extender was 87% (n = 15). When the semen was extended with the modified extender, centrifuged and stored, the pregnancy rate was 60% (n = 15). Inseminations were done every other day until ovulation was detected. If a mare ovulated more than 24 h after the last insemination, she was inseminated also after ovulation. The single-cycle pregnancy rate was 58% when the mares were inseminated only before ovulation (n = 19) but the rate was 100% when the inseminations were done both before and after ovulation (n = 9) or only after ovulation (n = 2). The difference in pregnancy rates was significant (p < 0.05), indicating that postovulatory inseminations probably serve to ensure the pregnancies. The extending and handling methods used in this study resulted in a combined pregnancy rate of 73%, and appear thus to be useful for storing stallion semen for approximately 2 days.
Get Full Text
Publication Date: 1994-01-01 PubMed ID: 7847194PubMed Central: PMC8101370DOI: 10.1186/BF03548330Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research studies the effects of different sperm extending methods and timing of insemination on the pregnancy rates in mares using slow-cooled, stored stallion semen. The results indicate a significant difference in pregnancy rates based on the extender used and the timing of insemination, particularly noting high success when insemination takes place both pre and post ovulation.

Research Methodology

  • The semen of three different stallions was drawn and extended using two methods, the Kenney extender and a version of this extender that was modified.
  • The extended semen was then cooled down gradually and stored for an average of 41 hours before it was used for insemination.
  • Each insemination dose contained 1.5-2 billion spermatozoa in a volume of 40ml.
  • In this study, 26 mares were inseminated over 30 cycles.

Findings

  • The pregnancy success rate for sperm stored with the traditional Kenney extender method was 87% over 15 instances.
  • When the semen was extended using the modified version of the Kenney extender, the success rate dropped to 60% over 15 instances, showing that the extending method significantly impacts the success rate.
  • In the study, pregnancy rates were also impacted by the timing of the insemination in relation to ovulation.
  • Mares inseminated exclusively before ovulation had a lower success rate at 58% over 19 instances. But the pregnancy rate increased to 100% with instances where inseminations occurred both before and after ovulation or only after ovulation.
  • This indicates that post-ovulatory inseminations likely have an important role in ensuring pregnancies.

Conclusion

  • The techniques used in this study, both in terms of semen extension and insemination timing, resulted in an overall pregnancy success rate of 73%, showing their effectiveness.
  • This suggests that these methods would be useful for storing stallion semen for approximately two days before insemination.

Cite This Article

APA
Heiskanen ML, Huhtinen M, Pirhonen A, Mäenpää PH. (1994). Insemination results with slow-cooled stallion semen stored for approximately 40 hours. Acta Vet Scand, 35(3), 257-262. https://doi.org/10.1186/BF03548330

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 35
Issue: 3
Pages: 257-262

Researcher Affiliations

Heiskanen, M L
  • Equine Research Station, Agricultural Research Centre, Ypäjä, Finland.
Huhtinen, M
    Pirhonen, A
      Mäenpää, P H

        MeSH Terms

        • Animals
        • Cryopreservation / methods
        • Cryopreservation / veterinary
        • Female
        • Horses / physiology
        • Insemination, Artificial / methods
        • Insemination, Artificial / veterinary
        • Male
        • Pregnancy
        • Semen Preservation / veterinary
        • Sperm Count / veterinary
        • Sperm Motility

        References

        This article includes 11 references
        1. de Vries PJ. Evaluation of the use of fresh, extended, transported stallion semen in the Netherlands. J. Reprod. Fert. 1987;35:641.
        2. Douglas-Hamilton DH, Osol R, Osol G, Driscoll D, Noble H. A field study of the fertility of transported equine semen.. Theriogenology 1984 Sep;22(3):291-304.
          doi: 10.1016/0093-691X(84)90486-2pubmed: 16725961google scholar: lookup
        3. Douglas-Hamilton MM, Viale K. Transported semen handbook for the stallion owner. 1984. p. 28.
        4. Francl AT, Amann RP, Squires EL, Pickett BW. Motility and fertility of equine spermatozoa in a milk extender after 12 or 24 hours at 20 degrees C.. Theriogenology 1987 Mar;27(3):517-25.
          doi: 10.1016/0093-691X(87)90239-1pubmed: 16726256google scholar: lookup
        5. Heiskanen ML, Pirhonen A, Koskinen E, Mäenpää PH. Motility and ATP content of extended equine spermatozoa in different storage conditions.. J Reprod Fertil Suppl 1987;35:103-7.
          pubmed: 3479564
        6. Jasko DJ, Hathaway JA, Schaltenbrand VL, Simper WD, Squires EL. Effect of seminal plasma and egg yolk on motion characteristics of cooled stallion spermatozoa. Theriogenology 1992;37:1241–1252.
          doi: 10.1016/0093-691X(92)90180-Ygoogle scholar: lookup
        7. Jasko DJ, Pickett BW, Amann RP. Effects of extenders and dilution rates on motility and fertility of cooled semen. Techniques for handling and utilization of transported equine spermatozoa and embryos 1991. pp. 17–31.
        8. Kenney RM, Bergman RV, Cooper WL, Morse GW. Minimal contamination techniques for breeding mares: Technique and preliminary findings. Proc. 21st Ann. Conv. A.A.E.P. 1975. pp. 327–335.
        9. Pickett BW, Sullivan JJ, Byers WW, Pace MM, Remmenga EE. Effect of centrifugation and seminal plasma on motility and fertility of stallion and bull spermatozoa.. Fertil Steril 1975 Feb;26(2):167-74.
          doi: 10.1016/S0015-0282(16)40938-6pubmed: 1126461google scholar: lookup
        10. van der Holst W. Stallion semen production in A. I. programs in the Netherlands. The Male in Farm Animal Production 1984. pp. 195–201.
        11. Zidane N, Vaillancourt P, Guay P, Bigras-Poulin M. Fertility of fresh equine semen preserved for up to 48 hours. J. Reprod. Fert. 1991;44:644.

        Citations

        This article has been cited 0 times.
        Subscribe to our newsletter
        Join 99,005 horse owners like you who receive our email updates on horse health and nutrition.