Insulin-like growth factor II in the horse: determination of a cDNA nucleotide sequence and expression in fetal and adult tissue.
Abstract: Horse cDNA for insulin-like growth factor II (IGF II) has been isolated. cDNA was synthesized from bulk mRNA and subsequently PCR-amplified and sequenced. Like its human counterpart, the mature horse IGF II peptide contains 67 amino acids with only two substitutions, isoleucine instead of valine in position 35 and asparagine instead of serine in position 36. The nucleotide homology was 92.1% between horse and human and 87.8% between horse and mouse. The isolated cDNA hybridized to multiple transcripts in fetal and adult tissues, thus confirming earlier reports on developmental expression of this gene in the horse.
Publication Date: 1994-11-01 PubMed ID: 7851727DOI: 10.1006/gcen.1994.1182Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research describes the identification and sequencing of horse cDNA for insulin-like growth factor II (IGF II), and its expression in fetal and adult horse tissue. The resulting horse IGF II peptide is similar but not identical to its human counterpart, and its DNA is significantly similar to both human and mouse versions.
Isolation and Sequencing of Horse cDNA for IGF II
- In their study, the researchers isolated the horse cDNA for insulin-like growth factor II (IGF II), a protein vital for growth and development.
- After the cDNA was synthesized from bulk mRNA, it was amplified by polymerase chain reaction (PCR), a common technique for making many copies of specific DNA segments, for further analysis.
- The sequence determination found that the mature horse IGF II peptide is made up of 67 amino acids, just like its human counterpart.
- However, there were two differences noticed in the sequence. The horse version has isoleucine in position 35 and asparagine in position 36, while the human version has valine and serine, respectively. These alterations have likely biological implications which were not detailed in the abstract.
Comparison of Nucleotide Homology and Expression in Tissues
- When comparing the nucleotide sequences between species, there was a 92.1% similarity between horse and human, and an 87.8% similarity between horse and mouse.
- Such high percentage values of homology indicate a strong evolutionary relationship and suggest similar functions of IGF II in these organisms.
- The researchers also found that the isolated horse cDNA for IGF II hybridized to multiple transcripts in both fetal and adult tissues. This means IGF II shows broad gene expression, i.e., it is manifest in various forms and stages of tissues.
- The discovery is consistent with previous research concerning the developmental expression of IGF II in horses, implying its critical role in horse growth and development.
Cite This Article
APA
Otte K, Engström W.
(1994).
Insulin-like growth factor II in the horse: determination of a cDNA nucleotide sequence and expression in fetal and adult tissue.
Gen Comp Endocrinol, 96(2), 270-275.
https://doi.org/10.1006/gcen.1994.1182 Publication
Researcher Affiliations
- Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cattle
- Cloning, Molecular
- DNA, Complementary
- Gene Expression
- Horses / genetics
- Humans
- Insulin-Like Growth Factor II / genetics
- Liver Extracts / metabolism
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction / methods
- Rats
- Sheep
- Swine
Citations
This article has been cited 5 times.- Kikuchi K, Sasaki K, Akizawa H, Tsukahara H, Bai H, Takahashi M, Nambo Y, Hata H, Kawahara M. Identification and expression analysis of cDNA encoding insulin-like growth factor 2 in horses. J Reprod Dev 2018 Feb 27;64(1):57-64.
- Nordin M, Bergman D, Halje M, Engström W, Ward A. Epigenetic regulation of the Igf2/H19 gene cluster. Cell Prolif 2014 Jun;47(3):189-99.
- Engström W, Shokrai A, Otte K, Granérus M, Gessbo A, Bierke P, Madej A, Sjölund M, Ward A. Transcriptional regulation and biological significance of the insulin like growth factor II gene. Cell Prolif 1998 Oct-Dec;31(5-6):173-89.
- Otte K, Choudhury D, Charalambous M, Engström W, Rozell B. A conserved structural element in horse and mouse IGF2 genes binds a methylation sensitive factor. Nucleic Acids Res 1998 Apr 1;26(7):1605-12.
- Raudsepp T, Otte K, Rozell B, Chowdhary BP. FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11. Mamm Genome 1997 Aug;8(8):569-72.
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