Integrated analysis of transcriptome and proteome for exploring mechanism of promoting proliferation of equine satellite cells associated with leucine.
Abstract: The proliferation and differentiation of skeletal muscle satellite cells (SCs) are necessary for the development of mature skeletal muscle. Leucine (Leu) is both an essential amino acid (EAA) and a branched-chain amino acid (BCAA), which has attracted worldwide attention due to its ability to repair and become new fibers. We separated the equine SCs into the control group (CON) and the Leu-supplemented group (LEU), which the cells were cultured in Leu-deprived and Leu-supplemented media respectively. We combined the transcriptome (RNA-Seq) and quantitative proteome (TMT) profiling analyses on proliferation of equine SCs associated with Leu. 1839 up-regulated and 631 down-regulated genes made up the 2470 differentially expressed genes (DEGs), and the 253 differentially abundant proteins (DEPs) included 118 up-regulated and 135 down-regulated proteins. 110 overlapping genes were verified based on the mRNA and protein translation relationship. Moreover, by comparing overlapped pathways through enrichment analysis, we found 13 genes not only appeared among 110 key DEGs/DEPs but also enriched in the KEGG overlapping signaling pathway, including CCL26, STAT2, PCK2, ASNS, GPT2, SHMT2, PHGDH, PGAM2, PSAT1, FTL, HMOX1, STEAP1 and STEAP2. To our knowledge, this is the first report in the world to systematically show how Leu regulated the growth of equine SCs. Leu deficiency inhibits the proliferation of equine SCs and development of fresh muscle fibers was proved in this paper. The main genes in charge of the Leu-induced proliferation of horse SCs have been found. These genes will make it easier to understand the mechanism at work and offer new information for enhancing the performance of sport horses and alleviating the equine muscle damage during exercise in the future.
Copyright © 2023. Published by Elsevier Inc.
Publication Date: 2023-08-19 PubMed ID: 37633218DOI: 10.1016/j.cbd.2023.101118Google Scholar: Lookup
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Summary
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This research study examines the role of leucine, an essential amino acid, in promoting the growth of skeletal muscle satellite cells in horses. The findings help to understand how leucine deficiency can inhibit cell proliferation and muscle fiber development, offering potential insights for improving equine performance and muscle health.
Methodology and Key Findings
- In the study, equine satellite cells (SCs) were divided into two groups–a control group (CON) and a leucine-supplemented group (LEU). The CON group was cultured in leucine-deprived media while the LEU group received leucine supplementation.
- The researchers conducted transcriptome (RNA-Seq) and quantitative proteome (TMT) profiling analyses in order to observe the effects of leucine on the proliferation of equine SCs.
- The result of these analyses showed 2470 differentially expressed genes (DEGs), out of which 1839 genes were up-regulated and 631 were down-regulated. Similarly, there were 253 differentially abundant proteins (DEPs), with 118 being up-regulated and 135 down-regulated.
- Among these, 110 overlapping genes were identified based on the mRNA and protein translation relationship, suggesting a significant role in leucine-induced cell proliferation.
Overlapping Signaling Pathways and Genes
- The researchers further analyzed the overlapping pathways of the key DEGs/DEPs through enrichment analysis. As a result, they found 13 genes which not only appeared among the 110 key genes but were also enriched in the KEGG overlapping signaling pathway.
- The identified genes were CCL26, STAT2, PCK2, ASNS, GPT2, SHMT2, PHGDH, PGAM2, PSAT1, FTL, HMOX1, STEAP1, and STEAP2.
Implications and Future Directions
- This research is the first of its kind to systematically investigate how leucine regulates the growth of equine satellite cells. The findings amply demonstrate that leucine deficiency can negatively impact cell proliferation and the development of new muscle fibers.
- The main genes responsible for leucine-induced proliferation of horse SCs have been identified, offering a greater understanding of the underlying mechanisms.
- The results of this study may provide crucial insights for the future enhancement of sports horse performance and the reduction of muscle damage during exercise, helping to maintain and improve equine health.
Cite This Article
APA
Xing J, Xie L, Qi X, Liu G, Akhtar MF, Li X, Bou G, Bai D, Zhao Y, Dugarjaviin M, Zhang X.
(2023).
Integrated analysis of transcriptome and proteome for exploring mechanism of promoting proliferation of equine satellite cells associated with leucine.
Comp Biochem Physiol Part D Genomics Proteomics, 48, 101118.
https://doi.org/10.1016/j.cbd.2023.101118 Publication
Researcher Affiliations
- Key Laboratory of Equus Germplasm Innovation (Co-const ruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China; College of animal science, Qingdao Agricultural University, Qingdao 266000, China.
- College of Agronomy, Liaocheng University, Shandong Engineering Technology Research Center for Efficient Breeding and Ecological Feeding of Black Donkey, Shandong Donkey Industry Technology Collaborative Innovation Center, Liaocheng 252059, Shandong Province, China.
- College of Agronomy, Liaocheng University, Shandong Engineering Technology Research Center for Efficient Breeding and Ecological Feeding of Black Donkey, Shandong Donkey Industry Technology Collaborative Innovation Center, Liaocheng 252059, Shandong Province, China.
- College of Agronomy, Liaocheng University, Shandong Engineering Technology Research Center for Efficient Breeding and Ecological Feeding of Black Donkey, Shandong Donkey Industry Technology Collaborative Innovation Center, Liaocheng 252059, Shandong Province, China.
- College of Agronomy, Liaocheng University, Shandong Engineering Technology Research Center for Efficient Breeding and Ecological Feeding of Black Donkey, Shandong Donkey Industry Technology Collaborative Innovation Center, Liaocheng 252059, Shandong Province, China.
- Key Laboratory of Equus Germplasm Innovation (Co-const ruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-const ruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-const ruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-const ruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-const ruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-const ruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address: zhangxinzhuang@imau.edu.cn.
Conflict of Interest Statement
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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