Interleukin-1-like activity in synovial fluids and sera of horses with arthritis.
Abstract: Synovial fluid samples of horses with osteoarthritis were investigated to detect interleukin-1 (IL-1) activity which could contribute to the disease pathogenesis. Of the 32 samples tested, 12 (37.5 per cent) showed an augmented phytohaemagglutinin induced proliferation of C3H/HeJ mouse thymocytes. Positive results were also seen in horses with infected arthritis, osteochondritis, traumatic arthritis and undefined synovial effusions. Normal synovial fluid and sera from all groups failed to show any detectable IL-1 activity. Fractionation of synovial fluid showed that the IL-1 activity was in the 15 to 20 Kd fractions. In the absence of mitogen, synovial fluid failed to stimulate thymocytes and did not stimulate the growth of an interleukin-2 (IL-2) dependent CTLL cell line, but synovial fluid stimulated IL-2 release by mouse spleen cells incubated with suboptimal doses of lectin. Evidence of an IL-1 inhibitor in synovial fluid from osteoarthritic horses was provided by ultrafiltration experiments and by the inhibitory activity of synovial fluid at particular dilutions in the thymocyte assay. The presence of IL-1-like activity could be relevant in the pathogenesis of arthritis in horses.
Publication Date: 1991-07-01 PubMed ID: 1896633DOI: 10.1016/0034-5288(91)90034-lGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article investigates the presence of Interleukin-1 (IL-1) activity in the synovial fluid of horses with osteoarthritis, considering its possible contribution to the development and progression of the disease.
Objective
The core objective of this research was to evaluate the prevalence of Interleukin-1 (IL-1) activity in the synovial fluid samples culled from horses diagnosed with osteoarthritis, and assess its potential role in osteoarthritis pathogenesis.
Methodology
- The team examined 32 synovial fluid samples sourced from the equine subjects.
- The study attempted to pinpoint the presence of Interleukin-1 by observing the increase in phytohaemagglutinin-induced proliferation of a particular type of mouse thymocytes (C3H/HeJ).
- In addition to osteoarthritis, the experiment also studied other types of arthritis like infected arthritis, osteochondritis, traumatic arthritis and cases with unclear synovial effusion.
Investigative Findings
- Out of the 32 samples, 12(37.5%) showed an augmented spur of C3H/HeJ mouse thymocytes, indicating the presence of Interleukin-1.
- Normal synovial fluid and sera showed no traceable IL-1 activity.
- Further fractionation of synovial fluid determined that the IL-1 activity resided in the 15 to 20 Kd fractions.
- Synovial fluid failed to stimulate thymocytes in the absence of mitogen, but was successful in stimulating the release of Interleukin-2 (IL-2) when combined with suboptimal doses of lectin.
- Ultrafiltration experiments and the inhibitory activity of synovial fluid conducted as part of this research lent evidence to the presence of an IL-1 inhibitor in the synovial fluid of the osteoarthritic horses.
Conclusions
The observations of this research is suggestive of the possibility that IL-1-like activity in the synovial fluid could be a relevant factor in the onset and pathogenesis of arthritis in horses. This opens new avenues for the understanding and potentially managing arthritis in equine subjects.
Cite This Article
APA
Alwan WH, Carter SD, Dixon JB, Bennett D, May SA, Edwards GB.
(1991).
Interleukin-1-like activity in synovial fluids and sera of horses with arthritis.
Res Vet Sci, 51(1), 72-77.
https://doi.org/10.1016/0034-5288(91)90034-l Publication
Researcher Affiliations
- Department of Veterinary Pathology, University of Liverpool.
MeSH Terms
- Animals
- Arthritis / immunology
- Arthritis / veterinary
- Chromatography, Gel
- Dose-Response Relationship, Immunologic
- Horse Diseases / immunology
- Horses
- Interleukin-1 / analysis
- Interleukin-1 / blood
- Lymphocyte Activation
- Mice
- Mice, Inbred C3H
- Osteoarthritis / immunology
- Osteoarthritis / veterinary
- Synovial Fluid / immunology
- T-Lymphocytes / immunology
Citations
This article has been cited 2 times.- Tung JT, Fenton JI, Arnold C, Alexander L, Yuzbasiyan-Gurkan V, Venta PJ, Peters TL, Orth MW, Richardson DW, Caron JP. Recombinant equine interleukin-1beta induces putative mediators of articular cartilage degradation in equine chondrocytes. Can J Vet Res 2002 Jan;66(1):19-25.
- Kearney CM, Korthagen NM, Plomp SGM, Labberté MC, de Grauw JC, van Weeren PR, Brama PAJ. A Translational Model for Repeated Episodes of Joint Inflammation: Welfare, Clinical and Synovial Fluid Biomarker Assessment. Animals (Basel) 2023 Oct 12;13(20).
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