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The Journal of investigative dermatology1990; 95(5); 582-586; doi: 10.1111/1523-1747.ep12505567

Interspecies and interregional analysis of the comparative histologic thickness and laser Doppler blood flow measurements at five cutaneous sites in nine species.

Abstract: Studies in dermatology, cutaneous pharmacology, and toxicology utilize skin from different animal species and body sites. However, regional differences exist in topical chemical percutaneous absorption studies in man and in animal. The objective of this study was to compare epidermal thickness and number of cell layers across species and body sites using both formalin-fixed paraffin and frozen sections. Cutaneous blood flow determined by laser Doppler velocimetry (LDV) was compared to histologic data. Six animals of each of the following species were used: monkeys, pigs, dogs, cats, cows, horses, rabbits, rats, and mice. Cutaneous blood flow was determined and 6-mm skin biopsies were taken directly from the following sites: buttocks, ear, humeroscapular joint, thoracolumbar junction, and abdominal area. When the two histologic methods were compared across all species and body sites, the thickness of the epidermis was significantly greater, and the thickness of the stratum corneum significantly less, in paraffin sections versus frozen sections (p less than 0.05). There were no differences in the number of viable cell layers determined by both methods. The values for LDV-determined blood flow did not significantly correlate (p greater than 0.05) to epidermal or stratum corneum thickness. However, regional and species differences were noted in all these parameters. In conclusion, these data indicate that thickness and LDV blood flow are independent and must be evaluated separately when comparisons are made between species and body sites. This work provides a data base for future comparative studies in which a knowledge of skin thickness or blood flow might be important variables.
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Publication Date: 1990-11-01 PubMed ID: 2230221DOI: 10.1111/1523-1747.ep12505567Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research demonstrates that there are significant differences in skin thickness and blood flow between animal species and body sites. Through comparison, researchers found that these two aspects are independent of each other and need to be evaluated separately for accurate results.

Objective and Methodology

  • The main objective of the study was to examine the variations in epidermal thickness and the number of cell layers across different species and body sites. Two methods were used in the experiment – the use of formalin-fixed paraffin and frozen sections.
  • The researchers collected data from six animals from each of the following different species: monkeys, pigs, dogs, cats, cows, horses, rabbits, rats, and mice.
  • 5 different body sites – including the buttocks, ear, humeroscapular joint, thoracolumbar junction, and the abdominal area – were chosen from which to take 6-mm skin biopsies and to measure cutaneous blood flow.

Results and Findings

  • A crucial finding was that the thickness of the epidermis was significantly greater and the thickness of the stratum corneum significantly less, in paraffin sections compared to frozen sections.
  • There were no differences in the number of viable cell layers determined by both methods.
  • The cutaneous blood flow as determined by laser Doppler velocimetry (LDV) did not significantly correlate to the thickness of either the epidermis or the stratum corneum.
  • However, variations were noted regarding skin thickness, cell layers, and blood flow among the different chosen body parts and species.

Conclusion

  • The data demonstrate that when comparing skin thickness and blood flow between different species and body regions, each must be assessed independently of the other.
  • This research provides valuable data that can be used in future comparative studies where skin thickness or blood flow might play a crucial role.

Cite This Article

APA
Monteiro-Riviere NA, Bristol DG, Manning TO, Rogers RA, Riviere JE. (1990). Interspecies and interregional analysis of the comparative histologic thickness and laser Doppler blood flow measurements at five cutaneous sites in nine species. J Invest Dermatol, 95(5), 582-586. https://doi.org/10.1111/1523-1747.ep12505567

Publication

The Journal of investigative dermatology
ISSN: 0022-202X
NlmUniqueID: 0426720
Country: United States
Language: English
Volume: 95
Issue: 5
Pages: 582-586

Researcher Affiliations

Monteiro-Riviere, N A
  • Cutaneous Pharmacology and Toxicology Center, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Bristol, D G
    Manning, T O
      Rogers, R A
        Riviere, J E

          MeSH Terms

          • Animals
          • Blood Flow Velocity / physiology
          • Cats
          • Cattle
          • Dogs
          • Horses
          • Macaca mulatta
          • Mice
          • Mice, Inbred Strains
          • Rabbits
          • Rats
          • Rats, Inbred Strains
          • Skin / cytology
          • Skin / diagnostic imaging
          • Skin Physiological Phenomena
          • Species Specificity
          • Swine
          • Ultrasonics
          • Ultrasonography

          Grant Funding

          • ES 00044 / NIEHS NIH HHS

          Citations

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