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Acta veterinaria Scandinavica2025; 67(1); 49; doi: 10.1186/s13028-025-00836-3

Intraligamentary anaesthesia: a local anaesthesia technique in equine dentistry.

Abstract: The injection of a local anaesthetic into the periodontal ligament (PDL) to achieve desensitisation of the pulp, periodontium, and adjacent tissues is a well-established technique in human dentistry, particularly in paediatric patients. This approach, commonly referred to as intraligamental or intraligamentary anaesthesia (ILA), has been widely adopted due to its effectiveness and relative simplicity. The aims of the present publication were: (1) to provide a review of the literature, (2) to describe an ILA technique adapted by the authors for use in equine dentistry, and (3) to evaluate this technique in an equine cadaver study. Results: Following injection of the solution into the periodontal ligament, the solution was observed to spread extensively through the PDL and alveolar bone, accumulating around the apex of the tooth. In horses, this distribution appeared to occur via both diffusion along the PDL within the periodontal space and infiltration into bone marrow spaces. Conclusions: The described ILA technique is straightforward to perform and can practically be applied in equine dental procedures. The cadaver study demonstrated consistent distribution of the injected solution around the root apex, supporting the anatomical basis for potential desensitisation of the pulp, periodontium, and surrounding tissues. While clinical studies are needed to confirm efficacy, these findings indicate that ILA may represent a useful additional technique for achieving local anaesthesia prior to tooth extraction in horses.
Publication Date: 2025-11-26 PubMed ID: 41299724PubMed Central: PMC12659135DOI: 10.1186/s13028-025-00836-3Google Scholar: Lookup
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Summary

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Overview

  • This study explores the use of intraligamentary anaesthesia (ILA), a technique where local anaesthetic is injected into the periodontal ligament (PDL), adapted from human dentistry for use in horses to achieve effective local anaesthesia during dental procedures.
  • The researchers reviewed existing literature, developed a specific ILA technique for equine dentistry, and tested it in a horse cadaver study to observe how the anaesthetic spreads around the tooth structures.

Background and Purpose

  • Intraligamentary Anaesthesia (ILA): A technique commonly used in human dentistry, especially for children, where anaesthetic is injected directly into the PDL to numb the pulp and surrounding tissues of a tooth.
  • Relevance to Equine Dentistry: Local anaesthesia is essential for pain management during equine dental procedures, such as tooth extractions, but traditional methods may have limitations.
  • Aims of This Study:
    • Conduct a literature review on ILA.
    • Describe a method tailored for equine patients to apply ILA effectively.
    • Evaluate the anatomical distribution of anaesthetic after ILA injection in horse cadavers to understand its potential as an effective technique.

Methodology

  • Technique Development: The authors adapted ILA injection procedures for the equine periodontal ligament, considering the anatomical differences between humans and horses.
  • Cadaver Study:
    • Local anaesthetic solution was injected into the PDL of horse cadaver teeth.
    • The spread of the anaesthetic was observed to understand how it distributes in equine periodontal and surrounding bone tissues.

Findings

  • Distribution of Anaesthetic:
    • The anaesthetic solution spread extensively through the PDL and alveolar bone of horse teeth.
    • It accumulated notably around the tooth apex, which is critical for effective numbing of the pulp and periodontium.
    • Two mechanisms of distribution were identified:
      • Diffusion along the PDL within the periodontal space.
      • Infiltration into bone marrow spaces in the alveolar bone.
  • Technical Feasibility:
    • The described ILA technique was straightforward and practical to perform in an equine dental context.

Conclusions and Implications

  • The study supports the anatomical basis for ILA to provide effective local anaesthesia in horses by showing consistent anaesthetic distribution in relevant dental tissues.
  • Given its simplicity and demonstrated spread pattern, ILA could be a valuable adjunctive technique for equine dentists, especially before procedures like tooth extraction.
  • However, clinical studies involving live horses are required to confirm the efficacy, pain control benefits, and safety of this technique in practice.
  • If validated, ILA could improve pain management options in equine dentistry and enhance animal welfare during dental treatment.

Cite This Article

APA
Teysen S, Demey W, Menzies R, Staszyk C, Birkhed D, Lundström T. (2025). Intraligamentary anaesthesia: a local anaesthesia technique in equine dentistry. Acta Vet Scand, 67(1), 49. https://doi.org/10.1186/s13028-025-00836-3

Publication

ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 67
Issue: 1
Pages: 49
PII: 49

Researcher Affiliations

Teysen, Stijn
  • DAP Vetrident, Geertskouter 32, Asse, 1730, Belgium. info@vetrident.be.
Demey, Wouter
  • DAP Equide, Stappersestraat 3, Schaffen, 3290, Belgium.
Menzies, Robert
  • Complete Veterinary Dental Care Pty Ltd, 132 Naroghid Road, Camperdown, VIC, 3260, Australia.
Staszyk, Carsten
  • Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Str. 98, 35392, Gießen, Germany.
Birkhed, Dowen
  • , Fersens Väg 14B, SE-211 42, Malmö, Sweden.
Lundström, Torbjörn
  • Djurtandvårdskliniken, Västra Husby, Norrköping, 605 96, Sweden.

MeSH Terms

  • Animals
  • Horses
  • Anesthesia, Local / veterinary
  • Anesthesia, Local / methods
  • Periodontal Ligament / drug effects
  • Cadaver
  • Anesthetics, Local / administration & dosage
  • Anesthesia, Dental / veterinary
  • Anesthesia, Dental / methods
  • Dentistry / veterinary
  • Dentistry / methods

Conflict of Interest Statement

Declarations. Ethics approval and consent to participate: This study did not require official or institutional ethical approval. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests.

References

This article includes 40 references
  1. Baghlaf K, Elashiry E, Alamoudi N. Computerized intraligamental anesthesia in children: A review of clinical considerations.. J Dent Anesth Pain Med 2018;18:197.
  2. Gupta A, Wadhwa J, Aggarwal V, Mehta N, Abraham D, Aneja K. Anesthetic efficacy of supplemental intraligamentary injection in human mandibular teeth with irreversible pulpitis: a systematic review and meta-analysis.. J Dent Anesth Pain Med 2022;22:1–10.
    doi: 10.17245/jdapm.2022.22.1.1pmc: PMC8814730pubmed: 35169615google scholar: lookup
  3. Pearce CJ. Recent developments in equine dentistry.. N Z Vet J 2020;68:178–86.
    doi: 10.1080/00480169.2020.1722971pubmed: 32028869google scholar: lookup
  4. Tremaine WH. Local analgesic techniques for the equine head.. Equine Vet Educ 2007;19:495–503.
    doi: 10.2746/095777307X207114google scholar: lookup
  5. Staszyk C, Bienert A, Bäumer W, Feige K, Gasse H. Simulation of local anaesthetic nerve block of the infraorbital nerve within the pterygopalatine fossa: anatomical landmarks defined by computed tomography.. Res Vet Sci 2008;85:399–406.
    doi: 10.1016/j.rvsc.2008.02.008pubmed: 18371997google scholar: lookup
  6. Tanner RB, Hubbell JAE. A retrospective study of the incidence and management of complications associated with regional nerve blocks in equine dental patients.. J Vet Dent 2019;36:40–5.
    doi: 10.1177/0898756419848165pubmed: 31138048google scholar: lookup
  7. Bach FS, Bodo G, Kuemmerle JM, Bienert-Zeit A, Hainisch EK, Simhofer H. Bacterial meningitis after sinus surgery in five adult horses.. Vet Surg 2014;43:697–703.
  8. Caldwell FJ, Easley KJ. Self-inflicted lingual trauma secondary to inferior alveolar nerve block in 3 horses.. Equine Vet Educ 2012;24:119–23.
  9. Calatayud J, González Á. History of the development and evolution of local anesthesia since the coca leaf.. Anesthesiology 2003;98:1503–8.
    pubmed: 12766665
  10. Eloesser L. Recent advances in regional (local) anesthesia.. Cal State J Med 1912;10:90–7.
    pmc: PMC1893651pubmed: 18735519
  11. Shabazfar N, Daubländer M, Al-Nawas B, Kämmerer PW. Periodontal intraligament injection as alternative to inferior alveolar nerve block-meta-analysis of the literature from 1979 to 2012.. Clin Oral Investig 2014;18(2):351–8.
    pubmed: 24077785
  12. Chompret L. Anesthésie par injections intraligamenteuses.. Rev Stomatol Chir Maxillofac 1920;6:309–31.
  13. Moore PA, Cuddy MA, Cooke MR, Sokolowski CJ. Periodontal ligament and intraosseous anesthetic injection techniques: alternatives to mandibular nerve blocks.. J Am Dent Assoc 2011;142:S13–8.
  14. Chenaux G, Castagnola L, Colombo A. L’anesthesie intraligamentaire avec La seringue (Peripress).. Schweiz Monatsschr Zahnheilkd 1976;86:1165–73.
    pubmed: 1070807
  15. Kaufman E, Galili D, Garfunkel AA. Intraligamentary anesthesia: A clinical study.. J Prosthet Dent 1983;49:337–9.
    doi: 10.1016/0022-3913(83)90273-1pubmed: 6573480google scholar: lookup
  16. Saoji H, Thomas Nainan M, Nanjappa N, Khairnar MR, Hishikar M, Jadhav V. Assessment of computer-controlled local anesthetic delivery system for pain control during restorative procedures: A randomized controlled trial.. J Dent Res Dent Clin Dent Prospects 2019;13:298–304.
    doi: 10.15171/joddd.2019.045pmc: PMC7072086pubmed: 32190214google scholar: lookup
  17. Hermans H, Veraa S, Wolschrijn CF, van Loon JPAM. Local anaesthetic techniques for the equine head, towards guided techniques and new applications.. Equine Vet Educ 2019;31:432–40.
    doi: 10.1111/eve.12757google scholar: lookup
  18. Lin S, Wigler R, Huber R, Kaufman AY. Anaesthetic efficacy of intraligamentary injection techniques on mandibular molars diagnosed with asymptomatic irreversible pulpitis: A retrospective study.. Aust Endod J 2017;43:34–7.
    doi: 10.1111/aej.12169pubmed: 27558847google scholar: lookup
  19. Kämmerer PW, Adubae A, Buttchereit I, Thiem DGE, Daubländer M, Frerich B. Prospective clinical study comparing intraligamentary anesthesia and inferior alveolar nerve block for extraction of posterior mandibular teeth.. Clin Oral Investig 2018;22:1469–75.
    doi: 10.1007/s00784-017-2248-2pubmed: 29034443google scholar: lookup
  20. Dalla Torre D, Burtscher D. Intraligamentary anaesthesia as a possible anaesthetic option in oral implantology: a retrospective analysis.. Int J Oral Maxillofac Surg 2020;49:811–5.
    doi: 10.1016/j.ijom.2019.10.017pubmed: 31740137google scholar: lookup
  21. Garfunkel AA, Kaufman E, Marmary Y, Galili D. Intraligamentary—intraosseous anesthesia: A radiographic demonstration.. Int J Oral Surg 1983;12:334–9.
    doi: 10.1016/S0300-9785(83)80022-2pubmed: 6420359google scholar: lookup
  22. Easley J, Dixon P, Schumacher J. Equine dentistry. Elsevier Ltd; 2010. p. 88.
  23. Yamada H, Maeda T, Hanada K, Takano Y. Reinnervation in the canine periodontal ligament of replanted teeth using an antibody to protein gene product 9.5: an immunohistochemical study.. Dent Traumatol 1999;15:221–34.
  24. Böhme G. Gehirnnerven. In: Nickel R, Schummer A, Seiferle E, editors. Lehrbuch der anatomie der Haustiere, Nervensystem, Sinnesorgane, endokrine Drüsen. 4th ed. Berlin: Parey; 2004.
  25. Meechan JG. How to overcome failed local anaesthesia.. Br Dent J 1999;186:15–20.
    doi: 10.1038/sj.bdj.4800006apubmed: 10028738google scholar: lookup
  26. Pradhan R, Kulkarni D, Shetty L. Evaluation of efficacy of intraligamentary injection technique for extraction of mandibular Teeth-A prospective study dentistry.. J Clin Diag Res 2017;11:110–3.
    pmc: PMC5324483pubmed: 28274058
  27. Ryalat ST, Al-Shayyab MH, Amin W, Alryalat SA, Al-Ryalat N, Sawair F. Efficacy of intraligamentary anesthesia in maxillary first molar extraction.. J Pain Res 2018;11:1829–33.
    doi: 10.2147/JPR.S170324pmc: PMC6140738pubmed: 30254485google scholar: lookup
  28. Froum SJ, Tarnow D, Caiazzo A, Hochman MN. Histologic response to intraligament injections using a computerized local anesthetic delivery System. A pilot study in Mini-Swine.. J Periodontol 2000;71:1453–9.
    doi: 10.1902/jop.2000.71.9.1453pubmed: 11022775google scholar: lookup
  29. Galili D, Kaufman E, Garfunkel AA, Michaeli Y. Intraligamentary anesthesia - a histological study.. Int J Oral Surg 1984;13:511–16.
    pubmed: 6439659
  30. Pertot WJ, Déjou J. Bone and root resorption. Effects of the force developed during periodontal ligament injections in dogs.. Oral Surg Oral Med Oral Pathol 1992;74:357–65.
    doi: 10.1016/0030-4220(92)90076-3pubmed: 1408000google scholar: lookup
  31. Roberts GJ, Holzel HS, Sury MRJ, Simmons NA, Gardner P, Longhurst P. Dental bacteremia in children.. Pediatr Cardiol 1997;18:24–7.
    doi: 10.1007/s002469900103pubmed: 8960488google scholar: lookup
  32. Kern I, Bartmann CP, Verspohl J, Rohde J, Bienert-Zeit A. Bacteraemia before, during and after tooth extraction in horses in the absence of antimicrobial administration.. Equine Vet J 2017;49:178–82.
    doi: 10.1111/evj.12581pubmed: 27062656google scholar: lookup
  33. Tsirlis AT, Iakovidis DP, Parissis NA. Dry socket: frequency of occurrence after intraligamentary anesthesia.. Quintessence Int (Berl) 1992;23:575–7.
    pubmed: 1410263
  34. Becker DE, Reed KL. Local anesthetics: review of Pharmacological considerations.. Anesth Prog 2012;59:90–102.
    doi: 10.2344/0003-3006-59.2.90pmc: PMC3403589pubmed: 22822998google scholar: lookup
  35. Ashkenazi M, Blumer S, Eli I. Effect of computerized delivery intraligamental injection in primary molars on their corresponding permanent tooth buds.. Int J Paediatr Dent 2010;20:270–5.
  36. Staszyk C, Wulff W, Jacob HG, Gasse H. Collagen fiber architecture of the periodontal ligament in equine cheek teeth.. J Vet Dent 2006;23:143–7.
    doi: 10.1177/089875640602300303pubmed: 17022193google scholar: lookup
  37. Kaufman E, LeResche L, Sommers E, Dworkin SF, Truelove EL. Intraligamentary anesthesia: a double-blind comparative study.. J Am Dent Assoc 1984;108:175–8.
  38. St George G, Morgan A, Meechan J, Moles DR, Needleman I, Ng YL. Injectable local anaesthetic agents for dental anaesthesia.. Cochrane Database Syst Rev 2018;10;7(7):CD006487.
    pmc: PMC6513572pubmed: 29990391
  39. Aggarwal V, Singla M, Miglani S, Kohli S, Sharma V, Bhasin SS. Does the volume of supplemental intraligamentary injections affect the anaesthetic success rate after a failed primary inferior alveolar nerve block? A randomized-double blind clinical trial.. Int Endod J 2018;51:5–11.
    doi: 10.1111/iej.12773pubmed: 28370327google scholar: lookup
  40. Burtscher D, Dalla Torre D. Intraligamentary anesthesia—a brief review of an underestimated anesthetic technique.. Oral Health Care 2019;4.
    doi: 10.15761/ohc.1000177google scholar: lookup

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