Intratesticular Versus Intrafunicular Lidocaine to Reduce Perioperative Nociception and Immunological Response in Ponies Undergoing Field Castration.

Vullo, C
·
Crupi, R
·
Di Paola, R
·
Cuzzocrea, S
·
Gugliandolo, E
·
Biondi, V
·
Catone, G

Abstract: The aim of this study was to evaluate the impact of intratesticular or intrafunicular lidocaine to reduce perioperative nociception and cytokine release in ponies undergoing field castration under total intravenous anaesthesia. Before castration, one group was injected with intrafunicular (FL) lidocaine and the other received intratesticular (TL) lidocaine. All ponies were premedicated with acepromazine (0.05 mg/kg) intramuscularly. Twenty minutes after the administration of acepromazine, xylazine (1 mg/kg) and butorphanol (0.02 mg/kg) were administered intravenously. Lidocaine 2% was given 1 mL/100 kg intrafunicularly in the FL groups or 2 mL/100 kg intratesticularly on each testicular side for TL. Surgery was performed by the same team of two experienced surgeons using Serra's emasculator and an open technique was used for all ponies in order to promote postoperative drainage. In this study, we focused on the plasmatic levels of TNF-α and IL-6. The results from this study showed a significant difference in plasmatic concentrations of TNF-α and IL-6 between the two different locoregional anaesthetic protocols. Taken together, the results suggest that the intrafunicular lidocaine locoregional anaesthesia could be a useful technique in the anaesthesia protocol for field pony castration.

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Publication Date: 2022-11-28 PubMed ID: 36548825PubMed Central: PMC9781484DOI: 10.3390/vetsci9120664Google Scholar: Lookup

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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the effects of intratesticular or intrafunicular lidocaine on pain and immune response in ponies during field castration under total intravenous anaesthesia. It found that intrafunicular lidocaine could be useful as part of the anaesthesia process.

Methodology

The study involved two groups of ponies undergoing field castration. One group received intrafunicular lidocaine (FL), while the other received intratesticular lidocaine (TL).
Before the castration, all ponies were given a premedication of acepromazine intramuscularly.
Twenty minutes after administering the acepromazine, the ponies were given an intravenous injection of xylazine and butorphanol.
Lidocaine 2% was then administered either intrafunicularly (1 mL/100 kg) in the FL group, or intratesticularly (2 mL/100 kg) on each testicular side for the TL group.
Same two experienced surgeons performed all the surgeries using an open technique to encourage postoperative drainage and they used Serra’s emasculator.

Focus and Findings

The study’s primary focus was on the plasmatic levels of TNF-α and IL-6, two important cytokines that play roles in immune response and inflammation.
The results showed that there was a considerable difference in the levels of TNF-α and IL-6 between the two groups.
This suggests that the injection location of the lidocaine can have an impact on the body’s immune response to post-surgical inflammation, as reflected by cytokine levels.

Conclusions

The findings of this study hint at the potential for intrafunicular lidocaine to be incorporated as part of the anaesthesia protocol for field pony castration.
Further implications include its possible benefits in decreasing postoperative pain and mitigating the immune response following surgery.

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APA

Vullo C, Crupi R, Di Paola R, Cuzzocrea S, Gugliandolo E, Biondi V, Catone G. (2022). Intratesticular Versus Intrafunicular Lidocaine to Reduce Perioperative Nociception and Immunological Response in Ponies Undergoing Field Castration. Vet Sci, 9(12), 664. https://doi.org/10.3390/vetsci9120664

Publication

Veterinary sciences

ISSN: 2306-7381

NlmUniqueID: 101680127

Country: Switzerland

Language: English

Volume: 9

Issue: 12

PII: 664

Researcher Affiliations

Vullo, Cecilia

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres, 31, 98166 Messina, Italy.

Crupi, Rosalia

Department of Veterinary Sciences, University of Messina, Polo SS. Annunziata, 98169 Messina, Italy.

Di Paola, Rosanna

Department of Veterinary Sciences, University of Messina, Polo SS. Annunziata, 98169 Messina, Italy.

Cuzzocrea, Salvatore

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres, 31, 98166 Messina, Italy.

Gugliandolo, Enrico

Department of Veterinary Sciences, University of Messina, Polo SS. Annunziata, 98169 Messina, Italy.

Biondi, Vito

Department of Veterinary Sciences, University of Messina, Polo SS. Annunziata, 98169 Messina, Italy.

Catone, Giuseppe

Department of Veterinary Sciences, University of Messina, Polo SS. Annunziata, 98169 Messina, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 34 references

Li YC, Xi CH, An YF, Dong WH, Zhou M. Perioperative inflammatory response and protein S-100u03b2 concentrations - relationship with post-operative cognitive dysfunction in elderly patients.. Acta Anaesthesiol Scand 2012 May;56(5):595-600.
doi: 10.1111/j.1399-6576.2011.02616.xpubmed: 22224444google scholar: lookup
Abdelmalak BB, You J, Kurz A, Kot M, Bralliar T, Remzi FH, Sessler DI. The effects of dexamethasone, light anesthesia, and tight glucose control on postoperative fatigue and quality of life after major noncardiac surgery: A randomized trial.. J Clin Anesth 2019 Aug;55:83-91.
doi: 10.1016/j.jclinane.2018.12.038pubmed: 30599425google scholar: lookup
Shoemaker R, Bailey J, Janzen E, Wilson DG. Routine castration in 568 draught colts: incidence of evisceration and omental herniation.. Equine Vet J 2004 May;36(4):336-40.
doi: 10.2746/0425164044890625pubmed: 15163041google scholar: lookup
Lemonnier LC, Thorin C, Meurice A, Dubus A, Touzot-Jourde G, Couroucu00e9 A, Leroux AA. Comparison of Flunixin Meglumine, Meloxicam and Ketoprofen on Mild Visceral Post-Operative Pain in Horses.. Animals (Basel) 2022 Feb 21;12(4).
doi: 10.3390/ani12040526pmc: PMC8868280pubmed: 35203234google scholar: lookup
Schumacher J. Complications of castration. Equine Vet. Educ. 1996;8:254u2013259. doi: 10.1111/j.2042-3292.1996.tb01700.x.
doi: 10.1111/j.2042-3292.1996.tb01700.xgoogle scholar: lookup
Kilcoyne I, Spier SJ. Castration Complications: A Review of Castration Techniques and How to Manage Complications.. Vet Clin North Am Equine Pract 2021 Aug;37(2):259-273.
doi: 10.1016/j.cveq.2021.04.002pubmed: 34116922google scholar: lookup
Searle D, Dart AJ, Dart CM, Hodgson DR. Equine castration: review of anatomy, approaches, techniques and complications in normal, cryptorchid and monorchid horses.. Aust Vet J 1999 Jul;77(7):428-34.
doi: 10.1111/j.1751-0813.1999.tb12083.xpubmed: 10451725google scholar: lookup
Kilcoyne I, Watson JL, Kass PH, Spier SJ. Incidence, management, and outcome of complications of castration in equids: 324 cases (1998-2008).. J Am Vet Med Assoc 2013 Mar 15;242(6):820-5.
doi: 10.2460/javma.242.6.820pubmed: 23445295google scholar: lookup
Abass M, Picek S, Garzu00f3n JFG, Ku00fchnle C, Zaghlou A, Bettschart-Wolfensberger R. Local mepivacaine before castration of horses under medetomidine isoflurane balanced anaesthesia is effective to reduce perioperative nociception and cytokine release.. Equine Vet J 2018 Nov;50(6):733-738.
doi: 10.1111/evj.12947pmc: PMC6175468pubmed: 29660154google scholar: lookup
Portier KG, Jaillardon L, Leece EA, Walsh CM. Castration of horses under total intravenous anaesthesia: analgesic effects of lidocaine.. Vet Anaesth Analg 2009 Mar;36(2):173-9.
doi: 10.1111/j.1467-2995.2008.00445.xpubmed: 19239656google scholar: lookup
Haga HA, Lykkjen S, Revold T, Ranheim B. Effect of intratesticular injection of lidocaine on cardiovascular responses to castration in isoflurane-anesthetized stallions.. Am J Vet Res 2006 Mar;67(3):403-8.
doi: 10.2460/ajvr.67.3.403pubmed: 16506900google scholar: lookup
Trindade PHE, Taffarel MO, Luna SPL. Spontaneous Behaviors of Post-Orchiectomy Pain in Horses Regardless of the Effects of Time of Day, Anesthesia, and Analgesia.. Animals (Basel) 2021 May 31;11(6).
doi: 10.3390/ani11061629pmc: PMC8230028pubmed: 34072875google scholar: lookup
Jordan SC, Choi J, Kim I, Wu G, Toyoda M, Shin B, Vo A. Interleukin-6, A Cytokine Critical to Mediation of Inflammation, Autoimmunity and Allograft Rejection: Therapeutic Implications of IL-6 Receptor Blockade.. Transplantation 2017 Jan;101(1):32-44.
doi: 10.1097/TP.0000000000001452pubmed: 27547870google scholar: lookup
Zhao YF, Yang HW, Yang TS, Xie W, Hu ZH. TNF-u03b1 - mediated peripheral and central inflammation are associated with increased incidence of PND in acute postoperative pain.. BMC Anesthesiol 2021 Mar 17;21(1):79.
doi: 10.1186/s12871-021-01302-zpmc: PMC7968228pubmed: 33730999google scholar: lookup
Esme H, Kesli R, Apiliogullari B, Duran FM, Yoldas B. Effects of flurbiprofen on CRP, TNF-u03b1, IL-6, and postoperative pain of thoracotomy.. Int J Med Sci 2011 Mar 10;8(3):216-21.
doi: 10.7150/ijms.8.216pmc: PMC3065790pubmed: 21448308google scholar: lookup
Heinrich PC, Behrmann I, Haan S, Hermanns HM, Mu00fcller-Newen G, Schaper F. Principles of interleukin (IL)-6-type cytokine signalling and its regulation.. Biochem J 2003 Aug 15;374(Pt 1):1-20.
doi: 10.1042/bj20030407pmc: PMC1223585pubmed: 12773095google scholar: lookup
Jawa RS, Anillo S, Huntoon K, Baumann H, Kulaylat M. Interleukin-6 in surgery, trauma, and critical care part II: clinical implications.. J Intensive Care Med 2011 Mar-Apr;26(2):73-87.
doi: 10.1177/0885066610384188pmc: PMC6223019pubmed: 21464062google scholar: lookup
Li QY, Xu HY, Yang HJ. [Effect of proinflammatory factors TNF-u03b1,IL-1u03b2, IL-6 on neuropathic pain].. Zhongguo Zhong Yao Za Zhi 2017 Oct;42(19):3709-3712.
doi: 10.19540/j.cnki.cjcmm.20170907.004pubmed: 29235283google scholar: lookup
Zhang H, Tai H, Ma Y, Li Y, Dang Z, Wang J, Zhao L. Postoperative Serum Levels of Interleukin-1u03b2 (IL-1u03b2), Interleukin-17 (IL-17), and Tumor Necrosis Factor-u03b1 (TNF-u03b1) in Patients Following Hip Replacement Surgery for Traumatic Fractured Femoral Neck: A Retrospective Study.. Med Sci Monit 2019 Aug 16;25:6120-6127.
doi: 10.12659/MSM.915369pmc: PMC6708283pubmed: 31417072google scholar: lookup
Pinho-Ribeiro FA, Verri WA Jr, Chiu IM. Nociceptor Sensory Neuron-Immune Interactions in Pain and Inflammation.. Trends Immunol 2017 Jan;38(1):5-19.
doi: 10.1016/j.it.2016.10.001pmc: PMC5205568pubmed: 27793571google scholar: lookup
Carroll CL, Huntington PJ. Body condition scoring and weight estimation of horses.. Equine Vet J 1988 Jan;20(1):41-5.
doi: 10.1111/j.2042-3306.1988.tb01451.xpubmed: 3366105google scholar: lookup
Vullo C, Carluccio A, Robbe D, Meligrana M, Petrucci L, Catone G. Guaiphenesin-ketamine-xylazine infusion to maintain anesthesia in mules undergoing field castration.. Acta Vet Scand 2017 Oct 11;59(1):67.
doi: 10.1186/s13028-017-0335-7pmc: PMC5637333pubmed: 29020956google scholar: lookup
Maimone S, Saffioti F, Filomia R, Caccamo G, Saitta C, Pallio S, Consolo P, Sabatini S, Sitajolo K, Franzu00e8 MS, Cacciola I, Raimondo G, Squadrito G. Elective endoscopic variceal ligation is not a risk factor for bacterial infection in patients with liver cirrhosis.. Dig Liver Dis 2018 Apr;50(4):366-369.
doi: 10.1016/j.dld.2017.12.012pubmed: 29317174google scholar: lookup
Calzetta L, Pistocchini E, Cito G, Ritondo BL, Verri S, Rogliani P. Inflammatory and contractile profile in LPS-challenged equine isolated bronchi: Evidence for IL-6 as a potential target against AHR in equine asthma.. Pulm Pharmacol Ther 2022 Jun;73-74:102125.
doi: 10.1016/j.pupt.2022.102125pubmed: 35351641google scholar: lookup
White FA, Bhangoo SK, Miller RJ. Chemokines: integrators of pain and inflammation.. Nat Rev Drug Discov 2005 Oct;4(10):834-44.
doi: 10.1038/nrd1852pmc: PMC2792904pubmed: 16224455google scholar: lookup
Baral P, Udit S, Chiu IM. Pain and immunity: implications for host defence.. Nat Rev Immunol 2019 Jul;19(7):433-447.
doi: 10.1038/s41577-019-0147-2pmc: PMC6700742pubmed: 30874629google scholar: lookup
Hess A, Axmann R, Rech J, Finzel S, Heindl C, Kreitz S, Sergeeva M, Saake M, Garcia M, Kollias G, Straub RH, Sporns O, Doerfler A, Brune K, Schett G. Blockade of TNF-u03b1 rapidly inhibits pain responses in the central nervous system.. Proc Natl Acad Sci U S A 2011 Mar 1;108(9):3731-6.
doi: 10.1073/pnas.1011774108pmc: PMC3048151pubmed: 21245297google scholar: lookup
Vazquez E, Kahlenbach J, Segond von Banchet G, Ku00f6nig C, Schaible HG, Ebersberger A. Spinal interleukin-6 is an amplifier of arthritic pain in the rat.. Arthritis Rheum 2012 Jul;64(7):2233-42.
doi: 10.1002/art.34384pubmed: 22246633google scholar: lookup
Impellizzeri D, Siracusa R, Cordaro M, Peritore AF, Gugliandolo E, Mancuso G, Midiri A, Di Paola R, Cuzzocrea S. Therapeutic potential of dinitrobenzene sulfonic acid (DNBS)-induced colitis in mice by targeting IL-1u03b2 and IL-18.. Biochem Pharmacol 2018 Sep;155:150-161.
doi: 10.1016/j.bcp.2018.06.029pubmed: 29963998google scholar: lookup
Cook AD, Christensen AD, Tewari D, McMahon SB, Hamilton JA. Immune Cytokines and Their Receptors in Inflammatory Pain.. Trends Immunol 2018 Mar;39(3):240-255.
doi: 10.1016/j.it.2017.12.003pubmed: 29338939google scholar: lookup
Cunha FQ, Poole S, Lorenzetti BB, Ferreira SH. The pivotal role of tumour necrosis factor alpha in the development of inflammatory hyperalgesia.. Br J Pharmacol 1992 Nov;107(3):660-4.
doi: 10.1111/j.1476-5381.1992.tb14503.xpmc: PMC1907751pubmed: 1472964google scholar: lookup
Costigan M, Scholz J, Woolf CJ. Neuropathic pain: a maladaptive response of the nervous system to damage.. Annu Rev Neurosci 2009;32:1-32.
doi: 10.1146/annurev.neuro.051508.135531pmc: PMC2768555pubmed: 19400724google scholar: lookup
Lotz M, Vaughan JH, Carson DA. Effect of neuropeptides on production of inflammatory cytokines by human monocytes.. Science 1988 Sep 2;241(4870):1218-21.
doi: 10.1126/science.2457950pubmed: 2457950google scholar: lookup
Chiu IM, von Hehn CA, Woolf CJ. Neurogenic inflammation and the peripheral nervous system in host defense and immunopathology.. Nat Neurosci 2012 Jul 26;15(8):1063-7.
doi: 10.1038/nn.3144pmc: PMC3520068pubmed: 22837035google scholar: lookup