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Home / Equine Research Bank / Pathogens / Investigation of an EHV-1 Outbreak in the United States Caus...
Pathogens (Basel, Switzerland)2021; 10(6); doi: 10.3390/pathogens10060747

Investigation of an EHV-1 Outbreak in the United States Caused by a New H752 Genotype.

Abstract: Here we report on an EHV-1 outbreak investigation caused by a novel genotype H752 (histidine in amino acid position 752 of the ORF 30 gene). The outbreak involved 31 performance horses. Horses were monitored over a period of 35 days for clinical signs, therapeutic outcome and qPCR results of EHV-1 in blood and nasal secretions. The morbidity of the EHV-1 outbreak was 84% with 26 clinically infected horses displaying fever and less frequently anorexia and distal limb edema. Four horses showed mild transient neurological deficits. Clinically diseased horses experienced high viral load of EHV-1 in blood and/or nasal secretions via qPCR, while subclinically infected horses had detectable EHV-1 mainly in nasal secretions. The majority of infected horses showed a rise in antibody titers to EHV-1 during the outbreak. All 31 horses were treated with valacyclovir, while clinically infected horses further received flunixin meglumine and sodium heparin. This investigation highlights various relevant aspects of an EHV-1 outbreak caused by a new H752 genotype: (i) importance of early detection of EHV-1 infection; (ii) diagnostic challenge to assess H752 genotype; (iii) apparent benefit of valacyclovir use in the early stage of the outbreak; and (iv) weekly testing of blood and nasal secretions by qPCR in order to monitor individual infection status and lift quarantine.
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Publication Date: 2021-06-13 PubMed ID: 34199153PubMed Central: PMC8231618DOI: 10.3390/pathogens10060747Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research discusses an outbreak of EHV-1, a disease affecting horses, caused by a new H752 genotype. The outbreak, which affected 31 performance horses, was monitored and investigated over a duration of 35 days.

About the Outbreak and the H752 Genotype

  • The outbreak investigated in this study was caused by EHV-1, an equine virus, and was novel in that it was linked to a new H (histidine) genotype located in amino acid position 752 of the enzyme gene.
  • The outbreak had a high morbidity rate with 84% of the 31 performance horses examined showing clinical signs of the infection.
  • The clinical signs included fever, anorexia, distal limb edema, and less frequently observed mild and transient neurological deficits.

Monitoring and Testing:

  • The horses were monitored over a period of 35 days for several factors, including clinical signs, therapeutic outcomes, and results of quantitative Polymerase Chain Reaction (qPCR) tests for EHV-1 in the horses’ blood and nasal secretions.
  • Clinically sick horses experienced a high viral load of EHV-1 detected in their blood and/or nasal secretions using qPCR.
  • Horses that were subclinically infected, which means they were infected but not displaying symptoms, had mainly detectable EHV-1 in their nasal secretions.

Therapeutic approach and Challenges:

  • All 31 horses were treated with an antiviral drug called Valacyclovir.
  • Horses that displayed clinical symptoms received additional treatments of non-steroidal anti-inflammatory drugs (Flunixin Meglumine) and blood thinners (Sodium Heparin).
  • Most of the horses showed an increase in antibodies to EHV-1, indicating an immune response to the infection.
  • The study raises a diagnostic challenge due to the difficulty to detect and diagnose the new H genotype.

Significance of the Study:

  • The study’s findings are crucial because they underline the importance of early detection of the EHV-1 infection, especially within outbreaks triggered by new genotypes like this one.
  • The study also notes the apparent benefit of using Valacyclovir in the early stages of the outbreak, as well as the importance of weekly testing of horses’ blood and nasal secretions using qPCR to monitor individual infection status and to guide decisions for lifting quarantine.

Cite This Article

APA
Pusterla N, Barnum S, Miller J, Varnell S, Dallap-Schaer B, Aceto H, Simeone A. (2021). Investigation of an EHV-1 Outbreak in the United States Caused by a New H752 Genotype. Pathogens, 10(6). https://doi.org/10.3390/pathogens10060747

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 10
Issue: 6

Researcher Affiliations

Pusterla, Nicola
  • Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Barnum, Samantha
  • Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Miller, Julia
  • Alliance Equine Health Care, Glenmoore, PA 19343, USA.
Varnell, Sarah
  • Alliance Equine Health Care, Glenmoore, PA 19343, USA.
Dallap-Schaer, Barbara
  • Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA 19348, USA.
Aceto, Helen
  • Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA 19348, USA.
Simeone, Aliza
  • Pennsylvania Department of Agriculture, Bureau of Animal Health, Collegeville, PA 17110, USA.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 24 references
  1. Ostlund EN. The equine herpesviruses. Vet. Clin. N. Am. Equine. Pract. 1993;9:283–294.
    doi: 10.1016/S0749-0739(17)30396-6pubmed: 8395324google scholar: lookup
  2. Henninger RW, Reed SM, Saville WJ, Allen GP, Hass GF, Kohn CW, Sofaly C. Outbreak of neurologic disease caused by equine herpesvirus-1 at a university equestrian center. J. Vet. Intern. Med. 2007;21:157–165.
    doi: 10.1111/j.1939-1676.2007.tb02942.xpubmed: 17338164google scholar: lookup
  3. Burgess BA, Tokateloff N, Manning S, Lohmann K, Lunn DP, Hussey SB, Morley PS. Nasal shedding of equine herpesvirus-1 from horses in an outbreak of equine herpes myeloencephalopathy in Western Canada. J. Vet. Intern. Med. 2012;26:384–392.
    doi: 10.1111/j.1939-1676.2012.00885.xpubmed: 22332764google scholar: lookup
  4. Traub-Dargatz JL, Pelzel-McCluskey AM, Creekmore LH, Geiser-Novotny S, Kasari TR, Wiedenheft AM, Bush EJ, Bjork KE. Case-control study of a multistate equine herpesvirus myeloencephalopathy outbreak. J. Vet. Intern. Med. 2013;27:339–346.
    doi: 10.1111/jvim.12051pubmed: 23398291google scholar: lookup
  5. McFadden AM, Hanlon D, McKenzie RK, Gibson I, Bueno IM, Pulford DJ, Orr D, Dunowska M, Stanislawek WL, Spence RP. The first reported outbreak of equine herpesvirus myeloencephalopathy in New Zealand. N. Z. Vet. J. 2016;64:125–134.
    doi: 10.1080/00480169.2015.1096853pubmed: 26414406google scholar: lookup
  6. Lesté-Lasserre C. Deadly viral outbreak ravages European horses. Science 2021;371:1297.
    doi: 10.1126/science.371.6536.1297pubmed: 33766865google scholar: lookup
  7. Nugent J, Birch-Machin I, Smith KC, Mumford JA, Swann Z, Newton JR, Bowden RJ, Allen GP, Davis-Poynter N. Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks. J. Virol. 2006;80:4047–4060.
    doi: 10.1128/JVI.80.8.4047-4060.2006pmc: PMC1440451pubmed: 16571821google scholar: lookup
  8. Perkins GA, Goodman LB, Tsujimura K, Van de Walle GR, Kim SG, Dubovi EJ, Osterrieder N. Investigation of the prevalence of neurologic equine herpes virus type 1 (EHV-1) in a 23-year retrospective analysis (1984-2007). Vet. Microbiol. 2009;139:375–378.
    doi: 10.1016/j.vetmic.2009.06.033pubmed: 19615831google scholar: lookup
  9. Sutton G, Thieulent C, Fortier C, Hue ES, Marcillaud-Pitel C, Pléau A, Deslis A, Guitton E, Paillot R, Pronost S. Identification of a new equid herpesvirus 1 DNA polymerase (ORF30) genotype with the isolation of a C(2254)/H(752) strain in French horses showing no major impact on the strain behaviour. Viruses 2020;12:1160.
    doi: 10.3390/v12101160pmc: PMC7650556pubmed: 33066315google scholar: lookup
  10. Goodman LB, Loregian A, Perkins GA, Nugent J, Buckles EL, Mercorelli B, Kydd JH, Palù G, Smith KC, Osterrieder N. A point mutation in a herpesvirus polymerase determines neuropathogenicity. PLoS Pathog. 2007;3:e160.
    doi: 10.1371/journal.ppat.0030160pmc: PMC2065875pubmed: 17997600google scholar: lookup
  11. Allen GP. Development of a real-time polymerase chain reaction assay for rapid diagnosis of neuropathogenic strains of equine herpesvirus-1. J. Vet. Diagn. Invest. 2007;19:69–72.
    doi: 10.1177/104063870701900110pubmed: 17459834google scholar: lookup
  12. Smith KL, Li Y, Breheny P, Cook RF, Henney PJ, Sells S, Pronost S, Lu Z, Crossley BM, Timoney PJ. New real-time PCR assay using allelic discrimination for detection and differentiation of equine herpesvirus-1 strains with A2254 and G2254 polymorphisms. J. Clin. Microbiol. 2012;50:1981–1988.
    doi: 10.1128/JCM.00135-12pmc: PMC3372139pubmed: 22493339google scholar: lookup
  13. Leutenegger CM, Madigan JE, Mapes S, Thao M, Estrada M, Pusterla N. Detection of EHV-1 neuropathogenic strains using real-time PCR in the neural tissue of horses with myeloencephalopathy. Vet. Rec. 2008;162:688–690.
    doi: 10.1136/vr.162.21.688pubmed: 18503069google scholar: lookup
  14. Balasuriya UB, Crossley BM, Timoney PJ. A review of traditional and contemporary assays for direct and indirect detection of equid herpesvirus 1 in clinical samples. J. Vet. Diagn. Invest. 2015;27:673–687.
    doi: 10.1177/1040638715605558pubmed: 26472746google scholar: lookup
  15. Maxwell LK, Bentz BG, Gilliam LL, Ritchey JW, Pusterla N, Eberle R, Holbrook TC, McFarlane D, Rezabek GB, Meinkoth J. Efficacy of the early administration of valacyclovir hydrochloride for the treatment of neuropathogenic equine herpesvirus type-1 infection in horses. Am. J. Vet. Res. 2017;78:1126–1139.
    doi: 10.2460/ajvr.78.10.1126pmc: PMC6440545pubmed: 28945127google scholar: lookup
  16. Shiraki K. Antiviral drugs against alphaherpesvirus. Adv. Exp. Med. Biol. 2018;1045:103–122.
    doi: 10.1007/978-981-10-7230-7_6pubmed: 29896665google scholar: lookup
  17. Estell KE, Dawson DR, Magdesian KG, Swain E, Laing ST, Siso S, Mapes S, Pusterla N. Quantitative molecular viral loads in 7 horses with naturally occurring equine herpesvirus-1 infection. Equine Vet. J. 2015;47:689–693.
    doi: 10.1111/evj.12351pubmed: 25212737google scholar: lookup
  18. Pusterla N, Hussey GS. Equine herpesvirus 1 myeloencephalopathy. Vet. Clin. N. Am. Equine Pract. 2014;30:489–506.
    doi: 10.1016/j.cveq.2014.08.006pubmed: 25300635google scholar: lookup
  19. Walter J, Seeh C, Fey K, Bleul U, Osterrieder N. Prevention of equine herpesvirus myeloencephalopathy—Is heparin a novel option? A case report. Tierarztl Prax Ausg G Grosstiere Nutztiere 2016;44:313–317.
    doi: 10.15653/TPG-150451pubmed: 27652372google scholar: lookup
  20. Thirumalapura N, Tewari D, Simeone A, Brightbill K, Anis E, Wheeler-Aceto H, Dallap-Schaer B. First reported detection of the equine herpesvirus 1 DNA polymerase 2254C/His752 variant in horses in the United States. J. Am. Vet. Med. Assoc. 2021;258:1181–1183.
    doi: 10.2460/javma.258.11.1181pubmed: 33978447google scholar: lookup
  21. Koppers-Lalic D, Reits EA, Ressing ME, Lipinska AD, Abele R, Koch J, Marcondes Rezende M, Admiraal P, van Leeuwen D, Bienkowska-Szewczyk K. Varicelloviruses avoid T cell recognition by UL49.5-mediated inactivation of the transporter associated with antigen processing. Proc. Natl. Acad. Sci. USA 2005;102:5144–5149.
    doi: 10.1073/pnas.0501463102pmc: PMC555605pubmed: 15793001google scholar: lookup
  22. van der Meulen KM, Favoreel HW, Pensaert MB, Nauwynck HJ. Immune escape of equine herpesvirus 1 and other herpesviruses of veterinary importance. Vet. Immunol. Immunopathol. 2006;111:31–40.
    doi: 10.1016/j.vetimm.2006.01.006pubmed: 16472872google scholar: lookup
  23. Pusterla N, Wilson WD, Mapes S, Finno C, Isbell D, Arthur RM, Ferraro GL. Characterization of viral loads, strain and state of equine herpesvirus-1 using real-time PCR in horses following natural exposure at a racetrack in California. Vet. J. 2009;179:230–239.
    doi: 10.1016/j.tvjl.2007.09.018pubmed: 18024200google scholar: lookup
  24. Gilkerson JR, Whalley JM, Drummer HE, Studdert MJ, Love DN. Epidemiological studies of equine herpesvirus 1 (EHV-1) in Thoroughbred foals: A review of studies conducted in the Hunter Valley of New South Wales between 1995 and 1997. Vet. Microbiol. 1999;68:15–25.
    doi: 10.1016/S0378-1135(99)00057-7pubmed: 10501158google scholar: lookup

Citations

This article has been cited 26 times.
  1. Mukhanbetkaliyev Y, Yessembekova G, Mukhanbetkaliyeva A, Akmambayeva B, Kadyrov A, Uskenov R, Bostanova S, Ashirbek A, Korennoy F, Abdrakhmanov S. Spatial Modeling of Equine Herpesviruses 1 (EHVs-1) Risks in Kazakhstan Using 2017-2024 Surveillance Data. Transbound Emerg Dis 2025;2025:5536099.
    doi: 10.1155/tbed/5536099pubmed: 40894192google scholar: lookup
  2. Durán MC, Suazo M, Maturana A, Vargas MP, García A, Ahumada C, Pezoa A, Goehring LS, Lara F. First Equine Herpes Myeloencephalopathy (EHM) Outbreak in Chile. Animals (Basel) 2025 Aug 11;15(16).
    doi: 10.3390/ani15162344pubmed: 40867672google scholar: lookup
  3. Musoles-Cuenca B, Padilla-Blanco M, Vitale V, Lorenzo-Bermejo T, de la Cuesta-Torrado M, Ballester B, Maiques E, Rubio-Guerri C, Velloso Alvarez A. First Molecular Evidence of Equine Herpesvirus Type 1 (EHV-1) in Ocular Swabs of Clinically Affected Horses. Viruses 2025 Jun 18;17(6).
    doi: 10.3390/v17060862pubmed: 40573453google scholar: lookup
  4. de la Cuesta-Torrado M, Vitale V, Velloso Alvarez A, Neira-Egea P, Diss C, Cuervo-Arango J. The Effect of Vaccination Status on Total Lymphocyte Count in Horses Affected by Equine Herpes Virus-1 Myeloencephalopathy. Animals (Basel) 2025 Apr 1;15(7).
    doi: 10.3390/ani15071019pubmed: 40218411google scholar: lookup
  5. de la Cuesta-Torrado M, Velloso Alvarez A, Santiago-Llorente I, Armengou L, Nieto F, Ríos J, Cruz-López F, Jose-Cunilleras E. Risk Factors and Long-Term Outcomes in Horses After the 2021 Outbreak of Equine Herpesvirus 1 Myeloencephalopathy, Valencia, Spain. J Vet Intern Med 2025 Mar-Apr;39(2):e70040.
    doi: 10.1111/jvim.70040pubmed: 40055829google scholar: lookup
  6. Pradhan SS, Balena V, Bera BC, Anand T, Khetmalis R, Madhwal A, Kandasamy S, Pavulraj S, Bernela M, Mor P, Tripathi BN, Virmani N. Multiple Gene Deletion Mutants of Equine Herpesvirus 1 Exhibit Strong Protective Efficacy Against Wild Virus Challenge in a Murine Model. Vaccines (Basel) 2025 Jan 8;13(1).
    doi: 10.3390/vaccines13010045pubmed: 39852824google scholar: lookup
  7. Mohamed E, Zarak I, Vereecke N, Theuns S, Laval K, Nauwynck H. Genomic analysis and replication kinetics of the closely related EHV-1 neuropathogenic 21P40 and abortigenic 97P70 strains. Vet Res 2025 Jan 13;56(1):12.
    doi: 10.1186/s13567-024-01434-3pubmed: 39806433google scholar: lookup
  8. Pusterla N, Lawton K, Barnum S, Ross K, Purcell K. Investigation of an Outbreak of Equine Herpesvirus-1 Myeloencephalopathy in a Population of Aged Working Equids. Viruses 2024 Dec 21;16(12).
    doi: 10.3390/v16121963pubmed: 39772269google scholar: lookup
  9. Hu Y, Zhang SY, Sun WC, Feng YR, Gong HR, Ran DL, Zhang BZ, Liu JH. Breaking Latent Infection: How ORF37/38-Deletion Mutants Offer New Hope against EHV-1 Neuropathogenicity. Viruses 2024 Sep 16;16(9).
    doi: 10.3390/v16091472pubmed: 39339948google scholar: lookup
  10. Khan A, Olajide E, Friedrich M, Holt A, Goehring LS. Evaluation of Non-Invasive Sampling Techniques for the Molecular Surveillance of Equid Herpesviruses in Yearling Horses. Viruses 2024 Jul 7;16(7).
    doi: 10.3390/v16071091pubmed: 39066254google scholar: lookup
  11. Öhrmalm J, Cholleti H, Theelke AK, Berg M, Gröndahl G. Divergent strains of EHV-1 in Swedish outbreaks during 2012 to 2021. BMC Vet Res 2024 Jun 22;20(1):270.
    doi: 10.1186/s12917-024-04096-7pubmed: 38909196google scholar: lookup
  12. de la Cuesta-Torrado M, Velloso Alvarez A, Neira-Egea P, Cuervo-Arango J. Long-term performance of show-jumping horses and relationship with severity of ataxia and complications associated with myeloencephalopathy caused by equine herpes virus-1. J Vet Intern Med 2024 May-Jun;38(3):1799-1807.
    doi: 10.1111/jvim.17070pubmed: 38609161google scholar: lookup
  13. Hassanien RT, Thieulent CJ, Carossino M, Li G, Balasuriya UBR. Modulation of Equid Herpesvirus-1 Replication Dynamics In Vitro Using CRISPR/Cas9-Assisted Genome Editing. Viruses 2024 Mar 6;16(3).
    doi: 10.3390/v16030409pubmed: 38543774google scholar: lookup
  14. Tong P, Yang E, Liu B, Tian S, Suo Y, Pan J, Dang Y, Palidan N, Jia C, Kuang L, Xie J. Identification of neuropathogenic Varicellovirus equidalpha1 as a potential cause of respiratory disease outbreaks among horses in North Xinjiang, China, from 2021-2023. BMC Vet Res 2024 Feb 27;20(1):77.
    doi: 10.1186/s12917-024-03925-zpubmed: 38413936google scholar: lookup
  15. Soboll-Hussey G, Dorman DC, Burgess BA, Goehring L, Gross P, Neinast C, Osterrieder K, Pusterla N, Lunn DP. Relationship between equine herpesvirus-1 viremia and abortion or equine herpesvirus myeloencephalopathy in domesticated horses: A systematic review. J Vet Intern Med 2024 May-Jun;38(3):1872-1891.
    doi: 10.1111/jvim.16948pubmed: 38069576google scholar: lookup
  16. Pusterla N, Dorman DC, Burgess BA, Goehring L, Gross M, Osterrieder K, Soboll Hussey G, Lunn DP. Viremia and nasal shedding for the diagnosis of equine herpesvirus-1 infection in domesticated horses. J Vet Intern Med 2024 May-Jun;38(3):1765-1791.
    doi: 10.1111/jvim.16958pubmed: 38069548google scholar: lookup
  17. Emelogu U, Lewin AC, Balasuriya UBR, Liu CC, Wilkes RP, Zhang J, Mills EP, Carter RT. Phylogenomic assessment of 23 equid alphaherpesvirus 1 isolates obtained from USA-based equids. Virol J 2023 Nov 29;20(1):278.
    doi: 10.1186/s12985-023-02248-zpubmed: 38031153google scholar: lookup
  18. White N, Pelzel-McCluskey A. Cross-Sectional Survey of Horse Owners to Assess Their Knowledge and Use of Biosecurity Practices for Equine Infectious Diseases in the United States. Animals (Basel) 2023 Nov 17;13(22).
    doi: 10.3390/ani13223550pubmed: 38003167google scholar: lookup
  19. Chau VQ, Kolb AW, Miller DL, Yannuzzi NA, Brandt CR. Phylogenetic and Genomic Characterization of Whole Genome Sequences of Ocular Herpes Simplex Virus Type 1 Isolates Identifies Possible Virulence Determinants in Humans. Invest Ophthalmol Vis Sci 2023 Jul 3;64(10):16.
    doi: 10.1167/iovs.64.10.16pubmed: 37450309google scholar: lookup
  20. Barbosa JD, Lins AMC, Bomjardim HDA, Silveira NDSES, Barbosa CC, Beuttemmuller EA, Brito MF, Salvarani FM. Equine Herpesvirus Type 1 Myeloencephalitis in the Brazilian Amazon. Animals (Basel) 2022 Dec 23;13(1).
    doi: 10.3390/ani13010059pubmed: 36611668google scholar: lookup
  21. Main SC, Brown LP, Melvin KR, Campagna SR, Voy BH, Castro HF, Strickland LG, Hines MT, Jacobs RD, Gordon ME, Ivey JLZ. Metabolomic Profiles in Starved Light Breed Horses during the Refeeding Process. Animals (Basel) 2022 Sep 21;12(19).
    doi: 10.3390/ani12192527pubmed: 36230267google scholar: lookup
  22. Pusterla N, Barnum S, Young A, Mendonsa E, Lee S, Hankin S, Brittner S, Finno CJ. Molecular Monitoring of EHV-1 in Silently Infected Performance Horses through Nasal and Environmental Sample Testing. Pathogens 2022 Jun 24;11(7).
    doi: 10.3390/pathogens11070720pubmed: 35889966google scholar: lookup
  23. Price D, Barnum S, Mize J, Pusterla N. Investigation of the Use of Non-Invasive Samples for the Molecular Detection of EHV-1 in Horses with and without Clinical Infection. Pathogens 2022 May 13;11(5).
    doi: 10.3390/pathogens11050574pubmed: 35631095google scholar: lookup
  24. Thieulent CJ, Sutton G, Toquet MP, Fremaux S, Hue E, Fortier C, Pléau A, Deslis A, Abrioux S, Guitton E, Pronost S, Paillot R. Oral Administration of Valganciclovir Reduces Clinical Signs, Virus Shedding and Cell-Associated Viremia in Ponies Experimentally Infected with the Equid Herpesvirus-1 C(2254) Variant. Pathogens 2022 May 4;11(5).
    doi: 10.3390/pathogens11050539pubmed: 35631060google scholar: lookup
  25. Carvelli A, Nielsen SS, Paillot R, Broglia A, Kohnle L. Clinical impact, diagnosis and control of Equine Herpesvirus-1 infection in Europe. EFSA J 2022 Apr;20(4):e07230.
    doi: 10.2903/j.efsa.2022.7230pubmed: 35414834google scholar: lookup
  26. Tong P, Duan R, Palidan N, Deng H, Duan L, Ren M, Song X, Jia C, Tian S, Yang E, Kuang L, Xie J. Outbreak of neuropathogenic equid herpesvirus 1 causing abortions in Yili horses of Zhaosu, North Xinjiang, China. BMC Vet Res 2022 Mar 1;18(1):83.
    doi: 10.1186/s12917-022-03171-1pubmed: 35232435google scholar: lookup
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