Investigation of the potential immunomodulatory effects of resveratrol on equine whole blood: An in vitro investigation.
Abstract: Horses affected with gastrointestinal conditions such as colic or colitis are at substantial risk for translocation of bacterial components such as lipopolysaccharide (LPS, endotoxin) from the gastrointestinal tract into circulation resulting in systemic inflammation and subsequent morbidity and mortality. Therefore, there is a need for effective preventive and treatment strategies aimed at minimizing the host's inflammatory reaction to these pathogen-associated molecular patterns (PAMPs) from gastrointestinal disease. Resveratrol (RES, trans-3,5,4'-trihydroxystilbene) is a phytoalexin commonly found in fruits and beverages, including red wine. Health benefits associated with the consumption of red wine have been attributed to RES. Resveratrol has been significantly shown to exert a powerful anti-inflammatory effect in laboratory animals subjected to experimental endotoxemia/sepsis. Therefore, the objective of this study was to determine in vitro whether RES had an inhibitory effect on the production of tumor necrosis factor (TNF) in cultivated whole blood (Cwb) following stimulation by PAMPs. We hypothesized that RES would inhibit TNF production in Cwb following stimulation by LPS or lipoteichoic acid (LTA). Production of TNF bioactivity in Cwb was measured in the presence of phosphate buffered saline (control), ethanol (solvent control), dexamethasone (anti-inflammatory control), LPS, LTA, and three different concentrations of RES. Both LPS and LTA stimulated TNF production, and addition of dexamethasone was inhibitory to this effect. An anti-inflammatory effect for RES was not demonstrated under the current experimental conditions. Further studies are required to characterize the effect of RES on the equine innate immune system during systemic inflammation.
Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.
Publication Date: 2016-03-31 PubMed ID: 27234544DOI: 10.1016/j.rvsc.2016.03.015Google Scholar: Lookup
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- Journal Article
Summary
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This research investigates whether resveratrol, a compound found in fruits and beverages like red wine, can reduce inflammatory responses in horses suffering from gastrointestinal conditions. However, under the applied experimental conditions, no anti-inflammatory effect was uncovered for resveratrol.
Background
- Horses impacted by gastrointestinal difficulties like colitis and colic have high chances for bacterial components such as lipopolysaccharide (LPS) to escape the gut into the bloodstream which brings about systemic inflammation and heightens illness and death rates.
- The study highlights the necessity for effective preventive and therapeutic measures aimed at reducing the host’s inflammatory response to these pathogen-associated molecular patterns (PAMPs) that are brought about by gastrointestinal diseases.
Role of Resveratrol
- Resveratrol (RES, trans-3,5,4′-trihydroxystilbene) is a phytoalexin found commonly in fruits and drinks, including red wine. The health benefits related with red wine consumption are often linked to RES.
- Resveratrol has been demonstrated to have a potent anti-inflammatory effect in laboratory animals exposed to experimental endotoxemia/sepsis.
Objective of Research
- The aim of this study was to examine in vitro (outside a living organism) if RES could inhibit the production of a protein called tumor necrosis factor (TNF) in cultivated whole blood following stimulation by PAMPs.
- The researchers hypothesized that RES would block TNF production in cultivated whole blood following stimulation by LPS or lipoteichoic acid (LTA).
Methodology and Results
- The bioactivity of TNF in cultivated whole blood was measured in the presence of various substances such as phosphate buffered saline (the control), ethanol (solvent control), dexamethasone (anti-inflammatory control), LPS, LTA, and three different concentrations of RES.
- Both LPS and LTA were found to stimulate TNF production while adding dexamethasone suppressed this effect.
- An anti-inflammatory effect for RES was not found under the current experimental set-up.
Conclusion
- Improvements in research design are required to understand the effect of RES on the equine innate immune system during systemic inflammation. The authors suggest that further studies are needed for this characterization.
Cite This Article
APA
Martin LM, Johnson PJ, Amorim JR, Honaker AR, Donaldson RS, DeClue AE.
(2016).
Investigation of the potential immunomodulatory effects of resveratrol on equine whole blood: An in vitro investigation.
Res Vet Sci, 106, 97-99.
https://doi.org/10.1016/j.rvsc.2016.03.015 Publication
Researcher Affiliations
- University of Missouri College of Veterinary Medicine, 900 East Campus Drive, Columbia, MO 65211, USA. Electronic address: martinlyn@missouri.edu.
- University of Missouri College of Veterinary Medicine, 900 East Campus Drive, Columbia, MO 65211, USA.
- University of Missouri College of Veterinary Medicine, 900 East Campus Drive, Columbia, MO 65211, USA.
- University of Missouri College of Veterinary Medicine, 900 East Campus Drive, Columbia, MO 65211, USA; Florida Fish and Wildlife Conservation Commission, Fish and Wildlife Research Institute, 100 8th Avenue Southeast, St. Petersburg, FL 33701, USA.
- University of Missouri College of Veterinary Medicine, 900 East Campus Drive, Columbia, MO 65211, USA.
- University of Missouri College of Veterinary Medicine, 900 East Campus Drive, Columbia, MO 65211, USA.
MeSH Terms
- Animals
- Anti-Inflammatory Agents, Non-Steroidal / pharmacology
- Cytokines / blood
- Gene Expression Regulation / drug effects
- Horses / blood
- Immunity, Innate / drug effects
- Inflammation / veterinary
- Lipopolysaccharides / pharmacology
- Resveratrol
- Sesquiterpenes
- Stilbenes / pharmacology
- Teichoic Acids / pharmacology
- Tumor Necrosis Factor-alpha / genetics
- Tumor Necrosis Factor-alpha / metabolism
- Phytoalexins
Citations
This article has been cited 3 times.- Brockmueller A, Sajeev A, Koklesova L, Samuel SM, Kubatka P, Büsselberg D, Kunnumakkara AB, Shakibaei M. Resveratrol as sensitizer in colorectal cancer plasticity. Cancer Metastasis Rev 2024 Mar;43(1):55-85.
- El-Ghareeb WR, Kishawy ATY, Anter RGA, Aboelabbas Gouda A, Abdelaziz WS, Alhawas B, Meligy AMA, Abdel-Raheem SM, Ismail H, Ibrahim D. Novel Antioxidant Insights of Myricetin on the Performance of Broiler Chickens and Alleviating Experimental Infection with Eimeria spp.: Crosstalk between Oxidative Stress and Inflammation. Antioxidants (Basel) 2023 Apr 28;12(5).
- Martin LM, Johnson PJ, Amorim JR, DeClue AE. Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study. Int J Mol Sci 2020 Feb 20;21(4).
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