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Veterinary microbiology2009; 139(3-4); 375-378; doi: 10.1016/j.vetmic.2009.06.033

Investigation of the prevalence of neurologic equine herpes virus type 1 (EHV-1) in a 23-year retrospective analysis (1984-2007).

Abstract: A single nucleotide polymorphism in the equine herpesvirus 1 (EHV-1) DNA polymerase gene (ORF30 A(2254) to G) has been associated with clinical signs of equine herpes myeloencephalopathy (EHM). The purpose of our study was to determine the odds ratio for this genetic marker and EHM using a panel of field isolates from North America collected over the past twenty-three years. EHV-1 isolates cultured at the Cornell University Animal Health Diagnostic Laboratory from 1984 to 2007 were retrieved along with their clinical histories. DNA was extracted from these EHV-1 cultures and allelic discrimination was performed using real-time PCR. The results were confirmed by sequencing of the target region in ORF30. PCR and sequencing were in 100% agreement and showed that 19 out of the 176 isolates had the ORF30 G(2254) allele (11%), of which 16 were EHM cases and 3 respiratory or abortion cases. The odds of having neurologic disease with the ORF30 G(2254) genotype were computed as 162 times greater than those with the opposite allele ORF30 A(2254) (95% confidence interval: 35-742). Despite this strong statistical significance, 24% (5/21) of horses with neurologic disease in our study population harbored the "non-neurologic" form of the allele (ORF30 A(2254)), suggesting that other factors may also contribute to the onset of EHM.
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Publication Date: 2009-06-26 PubMed ID: 19615831DOI: 10.1016/j.vetmic.2009.06.033Google Scholar: Lookup
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  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The article is a study aiming to determine the link between a particular genetic marker in the equine herpesvirus 1 (EHV-1) and its role in the manifestation of neurologic equine herpes myeloencephalopathy (EHM). The study conducted over 23 years found that the specific genetic variant significantly increased the likelihood of onset of EHM, although other factors were also suggested to contribute.

Research aim and methodology

  • The main agenda of the study was to investigate the correlation between a single nucleotide polymorphism (SNP) in the EHV-1 DNA polymerase gene (ORF30 A(2254) to G) and clinical presentation of EHM. The SNP is a specific genetic difference or mutation that can potentially influence the severity of a disease.
  • The researchers used a collection of EHV-1 isolates from field samples gathered from North America over a period of 23 years (1984-2007).
  • EHV-1 isolates that were cultured at the Cornell University Animal Health Diagnostic Laboratory were retrieved, including their respective clinical histories.
  • DNA was extracted from these isolates and allelic discrimination, a process that differentiates between different versions of a gene, was carried out using real-time PCR. The outcomes were confirmed by sequencing the target region in ORF30.

Research findings and conclusions

  • There was a 100% agreement between the PCR and sequencing results, indicating reliability of the observations. The study found that 19 out of 176 isolates harbored the ORF30 G(2254) allele, accounting for 11% of the cases. Of these, the majorities were EHM cases, but a few had respiratory or abortion occurrences.
  • The odds of developing neurologic disease were calculated to be 162 times higher in cases with the ORF30 G(2254) genotype as compared to those with the ORF30 A(2254) genotype.
  • Despite the strong statistical significance, it was observed that 24% (5 out of 21) of horses with neurological illnesses in the studied population had the “non-neurologic” form of the allele (ORF30 A(2254)). This suggested that the ORF30 A(2254) to G polymorphism is not the only determinant in the onset of EHM and other factors most likely have roles to play.

Cite This Article

APA
Perkins GA, Goodman LB, Tsujimura K, Van de Walle GR, Kim SG, Dubovi EJ, Osterrieder N. (2009). Investigation of the prevalence of neurologic equine herpes virus type 1 (EHV-1) in a 23-year retrospective analysis (1984-2007). Vet Microbiol, 139(3-4), 375-378. https://doi.org/10.1016/j.vetmic.2009.06.033

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 139
Issue: 3-4
Pages: 375-378

Researcher Affiliations

Perkins, Gillian A
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. gap7@cornell.edu
Goodman, Laura B
    Tsujimura, Koji
      Van de Walle, Gerlinde R
        Kim, Sung G
          Dubovi, Edward J
            Osterrieder, Nikolaus

              MeSH Terms

              • Abortion, Veterinary / epidemiology
              • Abortion, Veterinary / virology
              • Animals
              • DNA-Directed DNA Polymerase / genetics
              • Female
              • Herpesviridae Infections / epidemiology
              • Herpesviridae Infections / genetics
              • Herpesviridae Infections / veterinary
              • Herpesviridae Infections / virology
              • Herpesvirus 1, Equid / enzymology
              • Herpesvirus 1, Equid / genetics
              • Herpesvirus 1, Equid / isolation & purification
              • Horse Diseases / epidemiology
              • Horse Diseases / virology
              • Horses
              • Humans
              • Neurodegenerative Diseases / epidemiology
              • Neurodegenerative Diseases / veterinary
              • Neurodegenerative Diseases / virology
              • North America / epidemiology
              • Polymorphism, Single Nucleotide
              • Pregnancy
              • Retrospective Studies

              Citations

              This article has been cited 36 times.
              1. Black JB, Frampton AR. Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread. Front Vet Sci 2023;10:1165917.
                doi: 10.3389/fvets.2023.1165917pubmed: 37275614google scholar: lookup
              2. Thieulent CJ, Sutton G, Toquet MP, Fremaux S, Hue E, Fortier C, Pléau A, Deslis A, Abrioux S, Guitton E, Pronost S, Paillot R. Oral Administration of Valganciclovir Reduces Clinical Signs, Virus Shedding and Cell-Associated Viremia in Ponies Experimentally Infected with the Equid Herpesvirus-1 C(2254) Variant. Pathogens 2022 May 4;11(5).
                doi: 10.3390/pathogens11050539pubmed: 35631060google scholar: lookup
              3. Hu Y, Jia Q, Liu J, Sun W, Bao Z, Che C, Wu G, Fan B, Jarhen, Ran D. Molecular characteristics and pathogenicity of an equid alphaherpesvirus 1 strain isolated in China. Virus Genes 2022 Aug;58(4):284-293.
                doi: 10.1007/s11262-022-01910-ypubmed: 35567668google scholar: lookup
              4. Carvelli A, Nielsen SS, Paillot R, Broglia A, Kohnle L. Clinical impact, diagnosis and control of Equine Herpesvirus-1 infection in Europe. EFSA J 2022 Apr;20(4):e07230.
                doi: 10.2903/j.efsa.2022.7230pubmed: 35414834google scholar: lookup
              5. Tong P, Duan R, Palidan N, Deng H, Duan L, Ren M, Song X, Jia C, Tian S, Yang E, Kuang L, Xie J. Outbreak of neuropathogenic equid herpesvirus 1 causing abortions in Yili horses of Zhaosu, North Xinjiang, China. BMC Vet Res 2022 Mar 1;18(1):83.
                doi: 10.1186/s12917-022-03171-1pubmed: 35232435google scholar: lookup
              6. Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar C, Herskin M, Michel V, Miranda Chueca MÁ, Roberts HC, Padalino B, Pasquali P, Spoolder H, Ståhl K, Calvo AV, Viltrop A, Winckler C, Carvelli A, Paillot R, Broglia A, Kohnle L, Baldinelli F, Van der Stede Y. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus-1. EFSA J 2022 Jan;20(1):e07036.
                doi: 10.2903/j.efsa.2022.7036pubmed: 35035581google scholar: lookup
              7. Pusterla N, Barnum S, Miller J, Varnell S, Dallap-Schaer B, Aceto H, Simeone A. Investigation of an EHV-1 Outbreak in the United States Caused by a New H(752) Genotype. Pathogens 2021 Jun 13;10(6).
                doi: 10.3390/pathogens10060747pubmed: 34199153google scholar: lookup
              8. Vereecke N, Carnet F, Pronost S, Vanschandevijl K, Theuns S, Nauwynck H. Genome Sequences of Equine Herpesvirus 1 Strains from a European Outbreak of Neurological Disorders Linked to a Horse Gathering in Valencia, Spain, in 2021. Microbiol Resour Announc 2021 May 20;10(20).
                doi: 10.1128/MRA.00333-21pubmed: 34016681google scholar: lookup
              9. Laval K, Poelaert KCK, Van Cleemput J, Zhao J, Vandekerckhove AP, Gryspeerdt AC, Garré B, van der Meulen K, Baghi HB, Dubale HN, Zarak I, Van Crombrugge E, Nauwynck HJ. The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1. Front Microbiol 2021;12:662686.
                doi: 10.3389/fmicb.2021.662686pubmed: 33746936google scholar: lookup
              10. Sutton G, Thieulent C, Fortier C, Hue ES, Marcillaud-Pitel C, Pléau A, Deslis A, Guitton E, Paillot R, Pronost S. Identification of a New Equid Herpesvirus 1 DNA Polymerase (ORF30) Genotype with the Isolation of a C(2254)/H(752) Strain in French Horses Showing no Major Impact on the Strain Behaviour. Viruses 2020 Oct 13;12(10).
                doi: 10.3390/v12101160pubmed: 33066315google scholar: lookup
              11. Lecollinet S, Pronost S, Coulpier M, Beck C, Gonzalez G, Leblond A, Tritz P. Viral Equine Encephalitis, a Growing Threat to the Horse Population in Europe?. Viruses 2019 Dec 24;12(1).
                doi: 10.3390/v12010023pubmed: 31878129google scholar: lookup
              12. Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry. Front Microbiol 2019;10:2668.
                doi: 10.3389/fmicb.2019.02668pubmed: 31849857google scholar: lookup
              13. Preziuso S, Sgorbini M, Marmorini P, Cuteri V. Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes. Viruses 2019 Sep 13;11(9).
                doi: 10.3390/v11090851pubmed: 31540321google scholar: lookup
              14. Lechmann J, Schoster A, Ernstberger M, Fouché N, Fraefel C, Bachofen C. A novel PCR protocol for detection and differentiation of neuropathogenic and non-neuropathogenic equid alphaherpesvirus 1. J Vet Diagn Invest 2019 Sep;31(5):696-703.
                doi: 10.1177/1040638719871975pubmed: 31477001google scholar: lookup
              15. Garvey M, Lyons R, Hector RD, Walsh C, Arkins S, Cullinane A. Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017. Pathogens 2019 Jan 15;8(1).
                doi: 10.3390/pathogens8010007pubmed: 30650561google scholar: lookup
              16. Matczuk AK, Skarbek M, Jackulak NA, Bażanów BA. Molecular characterisation of equid alphaherpesvirus 1 strains isolated from aborted fetuses in Poland. Virol J 2018 Dec 3;15(1):186.
                doi: 10.1186/s12985-018-1093-5pubmed: 30509297google scholar: lookup
              17. Wimer CL, Schnabel CL, Perkins G, Babasyan S, Freer H, Stout AE, Rollins A, Osterrieder N, Goodman LB, Glaser A, Wagner B. The deletion of the ORF1 and ORF71 genes reduces virulence of the neuropathogenic EHV-1 strain Ab4 without compromising host immunity in horses. PLoS One 2018;13(11):e0206679.
                doi: 10.1371/journal.pone.0206679pubmed: 30440016google scholar: lookup
              18. Schnabel CL, Wimer CL, Perkins G, Babasyan S, Freer H, Watts C, Rollins A, Osterrieder N, Wagner B. Deletion of the ORF2 gene of the neuropathogenic equine herpesvirus type 1 strain Ab4 reduces virulence while maintaining strong immunogenicity. BMC Vet Res 2018 Aug 22;14(1):245.
                doi: 10.1186/s12917-018-1563-4pubmed: 30134896google scholar: lookup
              19. Tallmadge RL, Žygelytė E, Van de Walle GR, Kristie TM, Felippe MJB. Effect of a Histone Demethylase Inhibitor on Equine Herpesvirus-1 Activity In Vitro. Front Vet Sci 2018;5:34.
                doi: 10.3389/fvets.2018.00034pubmed: 29594155google scholar: lookup
              20. Stasiak K, Dunowska M, Hills SF, Rola J. Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland. Arch Virol 2017 Aug;162(8):2329-2335.
                doi: 10.1007/s00705-017-3376-3pubmed: 28451902google scholar: lookup
              21. Zhao J, Poelaert KCK, Van Cleemput J, Nauwynck HJ. CCL2 and CCL5 driven attraction of CD172a(+) monocytic cells during an equine herpesvirus type 1 (EHV-1) infection in equine nasal mucosa and the impact of two migration inhibitors, rosiglitazone (RSG) and quinacrine (QC). Vet Res 2017 Feb 27;48(1):14.
                doi: 10.1186/s13567-017-0419-4pubmed: 28241864google scholar: lookup
              22. Franz M, Goodman LB, Van de Walle GR, Osterrieder N, Greenwood AD. A Point Mutation in a Herpesvirus Co-Determines Neuropathogenicity and Viral Shedding. Viruses 2017 Jan 10;9(1).
                doi: 10.3390/v9010006pubmed: 28075374google scholar: lookup
              23. Wagner B, Perkins G, Babasyan S, Freer H, Keggan A, Goodman LB, Glaser A, Torsteinsdóttir S, Svansson V, Björnsdóttir S. Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age. PLoS One 2017;12(1):e0169072.
                doi: 10.1371/journal.pone.0169072pubmed: 28045974google scholar: lookup
              24. Stasiak K, Rola J, Ploszay G, Socha W, Zmudzinski JF. Detection of the neuropathogenic variant of equine herpesvirus 1 associated with abortions in mares in Poland. BMC Vet Res 2015 May 1;11:102.
                doi: 10.1186/s12917-015-0416-7pubmed: 25929692google scholar: lookup
              25. Marenzoni ML, Bietta A, Lepri E, Casagrande Proietti P, Cordioli P, Canelli E, Stefanetti V, Coletti M, Timoney PJ, Passamonti F. Role of equine herpesviruses as co-infecting agents in cases of abortion, placental disease and neonatal foal mortality. Vet Res Commun 2013 Dec;37(4):311-7.
                doi: 10.1007/s11259-013-9578-6pubmed: 24052369google scholar: lookup
              26. Walter J, Seeh C, Fey K, Bleul U, Osterrieder N. Clinical observations and management of a severe equine herpesvirus type 1 outbreak with abortion and encephalomyelitis. Acta Vet Scand 2013 Mar 5;55(1):19.
                doi: 10.1186/1751-0147-55-19pubmed: 23497661google scholar: lookup
              27. Yeo WM, Osterrieder N, Stokol T. Equine herpesvirus type 1 infection induces procoagulant activity in equine monocytes. Vet Res 2013 Mar 11;44(1):16.
                doi: 10.1186/1297-9716-44-16pubmed: 23497076google scholar: lookup
              28. Carmichael RJ, Whitfield C, Maxwell LK. Pharmacokinetics of ganciclovir and valganciclovir in the adult horse. J Vet Pharmacol Ther 2013 Oct;36(5):441-9.
                doi: 10.1111/jvp.12029pubmed: 23301502google scholar: lookup
              29. Smith KL, Li Y, Breheny P, Cook RF, Henney PJ, Sells S, Pronost S, Lu Z, Crossley BM, Timoney PJ, Balasuriya UB. New real-time PCR assay using allelic discrimination for detection and differentiation of equine herpesvirus-1 strains with A2254 and G2254 polymorphisms. J Clin Microbiol 2012 Jun;50(6):1981-8.
                doi: 10.1128/JCM.00135-12pubmed: 22493339google scholar: lookup
              30. Wagner B, Schnabel CL, Rollins A. Increase in Virus-Specific Mucosal Antibodies in the Upper Respiratory Tract Following Intramuscular Vaccination of Previously Exposed Horses Against Equine Herpesvirus Type-1/4. Vaccines (Basel) 2025 Mar 10;13(3).
                doi: 10.3390/vaccines13030290pubmed: 40266191google scholar: lookup
              31. Mohamed E, Zarak I, Vereecke N, Theuns S, Laval K, Nauwynck H. Genomic analysis and replication kinetics of the closely related EHV-1 neuropathogenic 21P40 and abortigenic 97P70 strains. Vet Res 2025 Jan 13;56(1):12.
                doi: 10.1186/s13567-024-01434-3pubmed: 39806433google scholar: lookup
              32. El Brini Z, Cullinane A, Garvey M, Fassi Fihri O, Fellahi S, Amraoui F, Loutfi C, Sebbar G, Paillot R, Piro M. First Molecular and Phylogenetic Characterization of Equine Herpesvirus-1 (EHV-1) and Equine Herpesvirus-4 (EHV-4) in Morocco. Animals (Basel) 2025 Jan 5;15(1).
                doi: 10.3390/ani15010102pubmed: 39795045google scholar: lookup
              33. Nishimura F, Fukushi N, Sakai H, Fukushi H. Attenuation of the neuropathogenic equine herpesvirus type 1 strain Ab4p in hamsters by a single amino acid mutation (D752N) in viral DNA polymerase ORF30. J Vet Med Sci 2024 Dec 1;86(12):1273-1278.
                doi: 10.1292/jvms.24-0338pubmed: 39384384google scholar: lookup
              34. Holmes CM, Babasyan S, Eady N, Schnabel CL, Wagner B. Immune horses rapidly increase antileukoproteinase and lack type I interferon secretion during mucosal innate immune responses against equine herpesvirus type 1. Microbiol Spectr 2024 Oct 3;12(10):e0109224.
                doi: 10.1128/spectrum.01092-24pubmed: 39162558google scholar: lookup
              35. Öhrmalm J, Cholleti H, Theelke AK, Berg M, Gröndahl G. Divergent strains of EHV-1 in Swedish outbreaks during 2012 to 2021. BMC Vet Res 2024 Jun 22;20(1):270.
                doi: 10.1186/s12917-024-04096-7pubmed: 38909196google scholar: lookup
              36. Tong P, Pan J, Dang Y, Yang E, Jia C, Duan R, Tian S, Palidan N, Kuang L, Wang C, Lu G, Xie J. First identification and isolation of equine herpesvirus type 1 in aborted fetal lung tissues of donkeys. Virol J 2024 May 27;21(1):117.
                doi: 10.1186/s12985-024-02390-2pubmed: 38802935google scholar: lookup
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