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Home / Equine Research Bank / Vet Microbiol / Investigation of the prevalence of neurologic equine herpes ...
Veterinary microbiology2009; 139(3-4); 375-378; doi: 10.1016/j.vetmic.2009.06.033

Investigation of the prevalence of neurologic equine herpes virus type 1 (EHV-1) in a 23-year retrospective analysis (1984-2007).

Abstract: A single nucleotide polymorphism in the equine herpesvirus 1 (EHV-1) DNA polymerase gene (ORF30 A(2254) to G) has been associated with clinical signs of equine herpes myeloencephalopathy (EHM). The purpose of our study was to determine the odds ratio for this genetic marker and EHM using a panel of field isolates from North America collected over the past twenty-three years. EHV-1 isolates cultured at the Cornell University Animal Health Diagnostic Laboratory from 1984 to 2007 were retrieved along with their clinical histories. DNA was extracted from these EHV-1 cultures and allelic discrimination was performed using real-time PCR. The results were confirmed by sequencing of the target region in ORF30. PCR and sequencing were in 100% agreement and showed that 19 out of the 176 isolates had the ORF30 G(2254) allele (11%), of which 16 were EHM cases and 3 respiratory or abortion cases. The odds of having neurologic disease with the ORF30 G(2254) genotype were computed as 162 times greater than those with the opposite allele ORF30 A(2254) (95% confidence interval: 35-742). Despite this strong statistical significance, 24% (5/21) of horses with neurologic disease in our study population harbored the "non-neurologic" form of the allele (ORF30 A(2254)), suggesting that other factors may also contribute to the onset of EHM.
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Publication Date: 2009-06-26 PubMed ID: 19615831DOI: 10.1016/j.vetmic.2009.06.033Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The article is a study aiming to determine the link between a particular genetic marker in the equine herpesvirus 1 (EHV-1) and its role in the manifestation of neurologic equine herpes myeloencephalopathy (EHM). The study conducted over 23 years found that the specific genetic variant significantly increased the likelihood of onset of EHM, although other factors were also suggested to contribute.

Research aim and methodology

  • The main agenda of the study was to investigate the correlation between a single nucleotide polymorphism (SNP) in the EHV-1 DNA polymerase gene (ORF30 A(2254) to G) and clinical presentation of EHM. The SNP is a specific genetic difference or mutation that can potentially influence the severity of a disease.
  • The researchers used a collection of EHV-1 isolates from field samples gathered from North America over a period of 23 years (1984-2007).
  • EHV-1 isolates that were cultured at the Cornell University Animal Health Diagnostic Laboratory were retrieved, including their respective clinical histories.
  • DNA was extracted from these isolates and allelic discrimination, a process that differentiates between different versions of a gene, was carried out using real-time PCR. The outcomes were confirmed by sequencing the target region in ORF30.

Research findings and conclusions

  • There was a 100% agreement between the PCR and sequencing results, indicating reliability of the observations. The study found that 19 out of 176 isolates harbored the ORF30 G(2254) allele, accounting for 11% of the cases. Of these, the majorities were EHM cases, but a few had respiratory or abortion occurrences.
  • The odds of developing neurologic disease were calculated to be 162 times higher in cases with the ORF30 G(2254) genotype as compared to those with the ORF30 A(2254) genotype.
  • Despite the strong statistical significance, it was observed that 24% (5 out of 21) of horses with neurological illnesses in the studied population had the “non-neurologic” form of the allele (ORF30 A(2254)). This suggested that the ORF30 A(2254) to G polymorphism is not the only determinant in the onset of EHM and other factors most likely have roles to play.

Cite This Article

APA
Perkins GA, Goodman LB, Tsujimura K, Van de Walle GR, Kim SG, Dubovi EJ, Osterrieder N. (2009). Investigation of the prevalence of neurologic equine herpes virus type 1 (EHV-1) in a 23-year retrospective analysis (1984-2007). Vet Microbiol, 139(3-4), 375-378. https://doi.org/10.1016/j.vetmic.2009.06.033

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 139
Issue: 3-4
Pages: 375-378

Researcher Affiliations

Perkins, Gillian A
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. gap7@cornell.edu
Goodman, Laura B
    Tsujimura, Koji
      Van de Walle, Gerlinde R
        Kim, Sung G
          Dubovi, Edward J
            Osterrieder, Nikolaus

              MeSH Terms

              • Abortion, Veterinary / epidemiology
              • Abortion, Veterinary / virology
              • Animals
              • DNA-Directed DNA Polymerase / genetics
              • Female
              • Herpesviridae Infections / epidemiology
              • Herpesviridae Infections / genetics
              • Herpesviridae Infections / veterinary
              • Herpesviridae Infections / virology
              • Herpesvirus 1, Equid / enzymology
              • Herpesvirus 1, Equid / genetics
              • Herpesvirus 1, Equid / isolation & purification
              • Horse Diseases / epidemiology
              • Horse Diseases / virology
              • Horses
              • Humans
              • Neurodegenerative Diseases / epidemiology
              • Neurodegenerative Diseases / veterinary
              • Neurodegenerative Diseases / virology
              • North America / epidemiology
              • Polymorphism, Single Nucleotide
              • Pregnancy
              • Retrospective Studies

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